May
In Vitro Experiments
5.6 Macrophages Starvation Treatment
5.6 S. Tm-macrophage Infection
5.6 Cell Condition Determination
5.9 Macrophages Starvation Treatment
5.9 Preparation of Culture Medium
5.9 Sterilization of Culture Medium
5.9 S. Tm-macrophage Infection
5.9 Determination of Reactive Oxygen Species (ROS) Content
5.9 Cell Lysis, Bacterial Suspension Dilution
5.10-5.11 Bacterial Colony Number Counting
5.13-5.14 Data and Results Analysis
5.19 Macrophages Starvation Treatment
5.19 Preparation of Culture Medium
5.19 Sterilization of Culture Medium
5.19 Pouring of Culture Medium
5.19 S. Tm-macrophage Infection
5.26 Macrophages Starvation Treatment
5.26 Preparation of Culture Medium
5.26 Sterilization of Culture Medium
5.26 Pouring of Culture Medium
5.26 S. Tm-macrophage Infection
June
In Vitro Experiments
6.2 Macrophages Starvation Treatment
6.2 Preparation of Culture Medium
6.2 Sterilization of Culture Medium
6.2 S. Tm-macrophage Infection
6.2 Determination of Reactive Oxygen Species (ROS) Content
6.2 Cell Lysis, Bacterial Suspension Dilution
6.3-6.4 Bacterial Colony Number Counting
In Vivo Experiment
July
Molecular Cloning(Both anti-inflammatory factors were done)
7.4 Connection between target gene and plasmid
7.4-7.5 Plasmid transformation
7.11-7.13 REPEAT
In Vivo Experiment
7.22-7.24 Establishment of colitis mouse model
7.29 Measurement of colon length in mice
7.29 lsolation of cells from intestinal lamina propria of mice
Augest
In Vivo Experiment
8.2 Immunofluorescence staining
8.12-8.14 Establishment of colitis mouse model
8.15-8.17 Engineering bacteria gavage
8.18 Measurement of colon length in mice
8.18 lsolation of cells from intestinal lamina propria of mice
8.18-8.21 Tissue embedding and sectioning
8.18 Flow cytometry detection in in vivo experiments