Experiments

In Vitro Experiment

Macrophage Thawing

Macrophage Passage

Macrophage Plating

S.Tm Liquid Culture

S.Tm Subculture

Macrophages Starvation Treatment

Preparation of Culture Medium

Sterilization of Culture Medium

Pouring of Culture Medium

S. Tm-macrophage Infection

Macrophage Collecting

Cell Number Counting

Determination of Reactive Oxygen Species (ROS) Content

Cell Lysis, Bacterial Suspension Dilution

Bacterial Plating

Bacterial Colony Number Counting

RNA Extraction

Reverse Transcription

RT-qPCR

Establishment of colitis mouse model

Peptide gavage

Engineering bacteria gavage

Determination of Adhesion Efficiency of Engineered Bacteria

cytometry detection in in vivo experiments

HE staining

Immunofluorescence staining

lsolation of cells from intestinal lamina propria of mice

Microbial diversity

Tissue embedding and sectioning

Target gene amplification

Plasmid enzyme digestion

Gel recycling

Connection between target gene and plasmid

Plasmid transformation

Plasmid extraction