BBa_K2507000 ThsS

BBa_K2507001 ThsR

ThsS and ThsR, both codon-optimized for E. coli, are two basic parts which belong to the two-component system from the marine bacterium Shewanella halifaxensis. ThsS is the membrane-bound sensor kinase (SK) which can sense thiosulfate outside the cell, and ThsR is the DNA-binding response regulator (RR)

BBa_K2507018 PphsA

PphsA(BBa_K2507018) is a ThsR-activated promoter which is turned on when ThsR is phosphorylated by ThsS after ThsS senses thiosulfate.

Because thiosulfate is a promising biomarker of inflammatory bowel disease, this system can be used as a sensor for inflammatory bowel disease diagnosing.

BBa_K5391004 TadA-CDd V106W

The TadA-CDd-V106W protein belongs to the CBE protein family. It was designed by members of David Liu's lab, who engineered fusions of CRISPR/Cas9 and a cytidine deaminase enzyme that retain the ability to be programmed with a guide RNA, do not induce dsDNA breaks, and mediate the direct conversion of cytidine to uridine, thereby effecting a C→T (or G→A) substitution.

Though as a Cytosine Base Editor，in addition to the risk of off-target DNA binding caused by traditional Cas9's non-specific interactions, the CBE system also has off-target risks that are independent of Cas9.But CBE what we use is evoluted from ABE by PANCE.So it is small, efficient and with low off-target activity which is totally different from simple CBE.And building upon the work from Liu's lab, we optimized the protein sequence, further reducing its off-target risk.

BBa_K5391002 Acanthocheilonema viteae cystatin(AvCystatin)

AvCystatin can regulate the immune response of macrophages and activates mitogen-activated protein (MAP) kinases in cells to induce IL-10 and IL-12/23p40 expression, which results in reduced gut inflammation.

BBa_K554008 HlyB

HlyB is part of the hemolysin secretion system, which is very important to export the proteins produced inside bacteria. In this exporting system, HlyB acts as an ATP-binding cassette, and recognizes the substrate via its secretion signal and is responsible for the specificity of the secretion system process. We choosed HlyB and HlyD to build up our exporting system to export our protein that produced inside Nissle 1917. BBa_K554007 HlyD

HlyD is part of the hemolysin secretion system, which is very important to export the proteins produced inside bacteria. In this exporting system, HlyD acts as a membrane fusion or adaptor protein. We choosed HlyB and HlyD to build up our exporting system to export our protein that produced inside Nissle 1917.

BBa_K1153000 Promoter norV (PnorV)

Promoter norV (PnorV) is the native promoter controlling the nitric oxide reduction operon in E. coli. It's transcriptional regulator, NorR, can bind to nitric oxide and activate gene expression.

BBa_K150001 CI protein

CI protein is a part of the lambda CI generator. The CI protein can specifically bind to the RBS sequence of the PL promoter, thereby inhibiting gene expression linked to the PL promoter.

BBa_K5391003 MazF

MazF is a toxin protein that will lead to cell apoptosis. Moreover, studies have shown that MazF has no toxin to healthy human cells so it can be used in vivo. Therefore, MazF could be an ideal biobrick used in the bacteria suicide switch.