BBa_K5391004 TadA-CDd V106W

The TadA-CDd-V106W protein belongs to the CBE protein family. It was designed by members of David Liu's lab, who engineered fusions of CRISPR/Cas9 and a cytidine deaminase enzyme that retain the ability to be programmed with a guide RNA, do not induce dsDNA breaks, and mediate the direct conversion of cytidine to uridine, thereby effecting a C→T (or G→A) substitution.

Though as a Cytosine Base Editor，in addition to the risk of off-target DNA binding caused by traditional Cas9's non-specific interactions, the CBE system also has off-target risks that are independent of Cas9.But CBE what we use is evoluted from ABE by PANCE.So it is small, efficient and with low off-target activity which is totally different from simple CBE.And building upon the work from Liu's lab, we optimized the protein sequence, further reducing its off-target risk.

BBa_K5391002 Acanthocheilonema viteae cystatin(AvCystatin)

AvCystatin can regulate the immune response of macrophages and activates mitogen-activated protein (MAP) kinases in cells to induce IL-10 and IL-12/23p40 expression, which results in reduced gut inflammation.

BBa_K5391003 MazF

MazF is a toxin protein that will lead to cell apoptosis. Moreover, studies have shown that MazF has no toxin to healthy human cells so it can be used in vivo. Therefore, MazF could be an ideal biobrick used in the bacteria suicide switch.