The PCR Reaction of DNA
1% Agarose Gel Electrophoresis
Gel Extraction and Purification
Gibson assemble
Golden Gate assemble
DNA transformation of E. coli
Colony PCR
Plasmids extraction
Enzyme Digestion Verification
RED homologous recombination
Growth Curve Verification
Caffeine-Based Growth Assay
Temperature-Dependent Phenotype Verification