Describe the research, experiments, and protocols you used in your iGEM project.
| Chemical | Manufacturer |
|---|---|
| MilliQ water Q-Pod® | Merck |
| DpnI | Jena Bioscience |
| BamHI | Jena Bioscience |
| SalI | Jena Bioscience |
| NheI | Jena Bioscience |
| AseI | NEB |
| 10X rCutsmart Buffer | NEB |
| 10X Universal Buffer | Jena Bioscience |
| NEB HiFi Assembly Master Mix | NEB |
| NEB positive control reaction mix | NEB |
| Agar | Fluka Chemie AG |
| LB (Luria/Miller) – Broth | Carl Roth |
| Kanamycin (50 mg/mL) | Carl Roth |
| Ampicillin (100 mg/mL) | Carl Roth |
| SOC Outgrowth Medium | NEB |
| 10X GC Buffer | NEB |
| 10mM dNTPs | PCR Biosystems |
| DMSO | NEB |
| Phusion-Polymerase | kind gift by Prof. Dr. Jan Faix |
| 96 – 100% Ethanol | Carl Roth |
| Glycerol | Sigma-Aldrich |
| DMEM, high glucose | Sigma-Aldrich |
| FCS | Sigma-Aldrich |
| Pen/Strep (Penicillin and Streptomycin) | Sigma-Aldrich |
| 0.25% Trypsin/EDTA | Gibco |
| DPBS | Sigma-Aldrich |
| GenJetTM Reagent (Ver. II) | SignaGen Laboratories |
| CuSO4 | Carl Roth |
| 1M HEPES | Carl Roth |
| FACS-Buffer (DPBS + 1%FCS) | Self-made |
| Consumable | Manufacturer |
|---|---|
| Pipets | Eppendorf |
| Pipet Tips | Sarstedt |
| Serological Pipets | Sarstedt |
| 1.5mL Reaction Tubes | Sarstedt |
| 2mL Reaction Tubes | Sarstedt |
| PCR-Tubes | Sarstedt |
| Cryo-Tubes | Sarstedt |
| 15mL Canonical Tubes | Sarstedt |
| 50mL Canocical Tubes | Sarstedt |
| Cell Culture Plates | Sarstedt |
| Petri Dishes, 100mm | Sarstedt |
| Zyppy™ Plasmid Miniprep Kit | Zymo Research |
| ZymoPURE II Plasmid Midiprep Kit | Zymo Research |
| HEK293T cells | DSMZ |
| NEB competent E. coli DH5-alpha cells | NEB |