Our project contributions cover a wide range from scientific communication to the creation of classic parts. Of the 22 parts submitted, three are codon-optimized genes that code for enzymes involved in the detection of antibiotics and heavy metals or in downstream signaling pathways. The other two parts are highly active promoters that are essential for the signaling. In addition, we have developed two computer-simulated models that are accessible via our wiki and can predict the effects of antibiotics and heavy metals on the development of resistance in bacteria. Another focus of our team was far-reaching scientific communication. We endeavoured to inform people of all ages about the dangers of antibiotics and heavy metals in water. To do this, we used interviews, social media posts and illustrated a children's book to convey this important topic in a vivid way.
We have registered a total of 22 new parts that can be used by other iGEM teams in the future. These include two reporter genes we used, as well as three codon-optimized genes for expression in human cells that encode the ß-lactam antibiotic-detecting enzyme and three transcription-activating enzymes. In addition to these basic parts, the corresponding composite parts were registered in which the gene was combined with a respective constitutive promoter and/ or reporter gene to track localization in the cell. For metal detection, we submitted a gene encoding a heavy metal detecting enzyme and its combination with a reporter gene as a composite part. In addition, we have developed four promoters, of which two are highly active, for both detection pathways to ensure efficient signal transduction.