| Registry Code | part name | Existing and new | Compound or basic | Type | Length(bp) | Group favorite |
| BBa_K5084000 | TtrS | New | Basic | Coding | 2016 | |
| BBa_K5084001 | TtrR | New | Basic | Coding | 621 | |
| BBa_K5084002 | PphsA | New | Basic | Regulatory | 342 | |
| BBa_K5084003 | Tetrathionate biosensor | New | Composite | Coding | 2780 | YES |
| BBa_J23105 | Pj23105 | Existing | Basic | Regulatory | 35 | |
| BBa_J23100 | Pj23100 | Existing | Basic | Regulatory | 35 | |
| BBa_K5084006 | Thiosulfate biosensor-A-RBS of thsS | New | Basic | RBS | 34 | |
| BBa_K5084007 | Thiosulfate biosensor-A-RBS of thsR | New | Basic | RBS | 33 | |
| BBa_K5084008 | Thiosulfate biosensor-B-RBS of thsS | New | Basic | RBS | 28 | |
| BBa_K5084009 | Thiosulfate biosensor-B-RBS of thsS | New | Basic | RBS | 33 | |
| BBa_K5084010 | ThsS | New | Basic | Coding | 1785 | |
| BBa_K5084011 | ThsR | New | Basic | Coding | 612 | |
| BBa_K5084012 | Thiosulfate biosensor-A | New | Composite | Coding | 4062 | YES |
| BBa_K5084013 | Thiosulfate biosensor-B | New | Composite | Coding | 4056 | YES |
| BBa_K5084024 | Thiosulfate biosensor-B-LacZα | New | Composite | Coding | 321 | YES |
| BBa_K5084015 | hlyB | New | Basic | Coding | 2124 | |
| BBa_K5084016 | hlyD | New | Basic | Coding | 1437 | |
| BBa_K5084017 | Hly-EGF | New | Basic | Coding | 807 | |
| BBa_K5084018 | Thiosulfate biosensor_Hly-EGF | New | Composite | Coding | 4159 | YES |
| BBa_K5084019 | Thiosulfate biosensor_Hly-EGF_α-Hemolysin | New | Composite | Coding | 7932 | YES |
| BBa_K5084020 | Rha-MazTox | New | Composite | Coding | YES | |
| BBa_K914003 | pRHa | Existing | Basic | Regulatory | 122 | YES |
This year, the GEC-Guangzhou team focused on tackling the challenging health issue of colitis, contributing 13 new basic parts and 7 new composite parts. Our project, titled "Colorful Poop: Genetically Engineered Probiotics Uncovering the Mysteries of Colitis," aims to revolutionize the detection and treatment of colitis by utilizing smart engineered probiotics. These probiotics are designed to sense inflammatory signals in the gut, turn blue for easy visual diagnosis, and release targeted therapeutic agents to treat the condition.
BBa_K5084000: TtrS, a coding sequence involved in sensing inflammatory signals related to colitis (2016 bp).
BBa_K5084001: TtrR, a coding sequence that works in tandem with TtrS for inflammation detection (621 bp).
BBa_K5084002: PphsA, a regulatory part controlling gene expression in response to inflammation (342 bp).
BBa_K5084006: Thiosulfate biosensor-A-RBS of thsS, a ribosome binding site (RBS) for the thsS gene in the thiosulfate biosensor system (34 bp).
BBa_K5084007: Thiosulfate biosensor-A-RBS of thsR, an RBS sequence for thsR gene expression (33 bp).
BBa_K5084008: Thiosulfate biosensor-B-RBS of thsS, a ribosome binding site for thsS in the alternate thiosulfate biosensor design (28 bp).
BBa_K5084009: Thiosulfate biosensor-B-RBS of thsS, another RBS sequence for thsS (33 bp).
BBa_K5084010: ThsS, a coding sequence responsible for thiosulfate sensing in the biosensor (1785 bp).
BBa_K5084011: ThsR, coding sequence for signal relay in the thiosulfate biosensor system (612 bp).
BBa_K5084015: hlyB, a coding sequence for the secretion system that enables therapeutic delivery in engineered probiotics (2124 bp).
BBa_K5084016: hlyD, another component of the secretion system for enhanced delivery of therapeutic agents (1437 bp).
BBa_K5084017: Hly-EGF, a fusion protein that combines hemolysin secretion system with epidermal growth factor (EGF) to promote tissue repair (807 bp).
BBa_K5084003: Tetrathionate biosensor, a composite coding part that senses tetrathionate, which is elevated in inflammatory bowel diseases, helping in early detection (2780 bp).
BBa_K5084012: Thiosulfate biosensor-A, a composite part designed to sense thiosulfate, an inflammation-related molecule, and trigger a response in engineered probiotics (4062 bp).
BBa_K5084013: Thiosulfate biosensor-B, a similar composite part for thiosulfate detection with a slightly different regulatory system (4056 bp).
BBa_K5084018: Thiosulfate biosensor_Hly-EGF, a composite part combining thiosulfate sensing with the Hly-EGF secretion system for both diagnosis and treatment (4159 bp).
BBa_K5084019: Thiosulfate biosensor_Hly-EGF_α-Hemolysin, an advanced composite part that integrates both inflammation sensing and the release of therapeutic agents for tissue repair (7932 bp).
BBa_K5084020: Rha-MazTox, a composite part that can induce bacterial lysis as a safety mechanism once inflammation levels decrease, ensuring controlled probiotic activity.
BBa_K5084024: Thiosulfate biosensor-B-LacZα, a composite part that includes a colorimetric reporter, allowing visual detection of inflammation through blue color changes (321 bp).
Our work introduces a novel approach for the detection and treatment of colitis using engineered probiotics. By integrating biosensor systems with therapeutic protein secretion, we open new possibilities for precision medicine. The design of colorimetric biosensors, which enable non-invasive diagnosis through stool color changes, combined with localized drug delivery, provides a valuable platform for future iGEM teams to expand upon in the fields of inflammatory diseases, biosafety, and probiotic therapies. Additionally, our work on safety mechanisms like bacterial lysis systems (Rha-MazTox) ensures that future applications will be both effective and safe for patients.
This project not only represents a significant advancement in colitis treatment but also highlights the power of synthetic biology in addressing real-world health challenges.