Welcome to the B.A.R.B.I.E Project’s Wet Lab Notebook! This document meticulously chronicles all the experimental techniques, findings, and analyses conducted by our team. It outlines the project’s key stages, covering each cycle of investigation, including bacterial transformations, protein expression, purification procedures, and more. This notebook offers insights into the methodologies we employed, the challenges we faced, and the breakthroughs we achieved throughout our iGEM project. We hope you enjoy exploring our scientific journey!