Parts

1. Basic Parts

Part Accession	Part Name	Type	Length(bp)	Favorite	Success
BBa_K5217000	pGPM1	Promoter	760	+++++	worked
BBa_K5217001	pINO1	Promoter	894	++++++	worked
BBa_K5217002	pTDH3	Promoter	667	++++	worked
BBa_K5217003	pGAL1	Promoter	451	+++	worked
BBa_K5217004	PGK1t	Terminator	271	+++++	worked
BBa_K5217005	TEF1t	Terminator	180	+++++	worked
BBa_K5217006	ADH1t	Terminator	187	+++++	worked
BBa_K5217007	tLsCl	CDS	1662	+++	plasmid constructed
BBa_K5217008	AmMYS	CDS	1743	++++	plasmid constructed
BBa_K5217009	CcOCS	CDS	1788	++++++	success
BBa_K5217010	AcNES1	CDS	1719	++++++++++	success
BBa_K5217011	StrCinS	CDS	990	++++++	plasmid constructed
BBa_K5217012	CbTPS1	CDS	1716	++++	plasmid constructed
BBa_K5217013	PgfB	CDS	1008	+++++++	success
BBa_K5217014	HpyCins	CDS	116	+++++++	worked

2. Composite Parts

Part Accession	Part Name	Type	Length(bp)	Favorite	Success	Description
BBa_K5217015	pINO1_tLsCl_TEF1t	composite	BBa_K5217001 BBa_K5217007 BBa_K5217005	+++++	plasmid constructed	pINO1 is a promoter. It is added before the gene tLsCl and followed by the TEF1 terminator.
BBa_K5217016	pGPM1_tLsCl_PGK1t	composite	BBa_K5217000 BBa_K5217007 BBa_K5217004	+++++	plasmid constructed	pGPM1 is a promoter. It is added before the gene tLsCl and followed by the PGK1 terminator.
BBa_K5217035	pTDH3_tLsCl_ADH1t	composite	BBa_K5217002 BBa_K5217007 BBa_K5217006	++++++	products not detected	pTDH3 is a promoter. It is added before the gene tLsCl and followed by the ADH1 terminator.
BBa_K5217018	pGPM1_CcOCS_PGK1t	composite	BBa_K5217000 BBa_K5217009 BBa_K5217004	++++++	plasmid constructed	pGPM1 is a promoter. It is added before the gene CcOCS and followed by the PGK1 terminator.
BBa_K5217019	pINO1_CcOCS_TEF1t	composite	BBa_K5217001 BBa_K5217009 BBa_K5217005	+++++++	success	pINO1 is a promoter. It is added before the gene CcOCS and followed by the TEF1 terminator.
BBa_K5217036	pTDH3_CcOCS_ADH1t	composite	BBa_K5217002 BBa_K5217009 BBa_K5217006	+++++++	success	pTDH3 is a promoter. It is added before the gene CcOCS and followed by the ADH1 terminator.
BBa_K5217021	pINO1_HpyCins_TEF1t	composite	BBa_K5217001 BBa_K5217014 BBa_K5217005	++++++++	success	pINO1 is a promoter. It is added before the gene HpyCins and followed by the TEF1 terminator.
BBa_K5217037	pTDH3_HpyCins_ADH1t	composite	BBa_K5217002 BBa_K5217014 BBa_K5217006	++++++++	success	pTDH3 is a promoter. It is added before the gene HpyCins and followed by the ADH1 terminator.
BBa_K5217023	pGPM1_StrCins_PGK1t	composite	BBa_K5217000 BBa_K5217011 BBa_K5217004	++++++	plasmid constructed	pGPM1 is a promoter. It is added before the gene StrCins and followed by the PGK1 terminator.
BBa_K5217024	pINO1_AcNES1_TEF1t	composite	BBa_K5217001 BBa_K5217010 BBa_K5217005	++++++	plasmid constructed	pINO1 is a promoter. It is added before the gene AcNES1 and followed by the TEF1 terminator.
BBa_K5217025	pGPM1_AcNES1_PGK1t	composite	BBa_K5217000 BBa_K5217010 BBa_K5217004	++++++++++	success	pGPM1 is a promoter. It is added before the gene AcNES1 and followed by the PGK1 terminator.
BBa_K5217026	pGAL1_AcNES1_PGK1t	composite	BBa_K5217003 BBa_K5217010 BBa_K5217004	++++++++++	success	pGAL1 is a promoter. It is added before the gene AcNES1 and followed by the PGK1 terminator.
BBa_K5217038	pTDH3_AcNES1_ADH1t	composite	BBa_K5217002 BBa_K5217010 BBa_K5217006	++++++++++	success	pTDH3 is a promoter. It is added before the gene AcNES1 and followed by the ADH1 terminator.
BBa_K5217028	pINO1_PgfB_TEF1t	composite	BBa_K5217001 BBa_K5217013 BBa_K5217005	+++++++	products not detected	pINO1 is a promoter. It is added before the gene PgfB and followed by the TEF1 terminator.
BBa_K5217029	pGPM1_PgfB_PGK1t	composite	BBa_K5217000 BBa_K5217013 BBa_K5217004	+++++++	products not detected	pGPM1 is a promoter. It is added before the gene PgfB and followed by the PGK1 terminator.
BBa_K5217039	pTDH3_PgfB_ADH1t	composite	BBa_K5217002 BBa_K5217010 BBa_K5217006	++++++++	success	pTDH3 is a promoter. It is added before the gene PgfB and followed by the ADH1 terminator.
BBa_K5217031	pGAL1_PgfB_PGK1t	composite	BBa_K5217003 BBa_K5217013 BBa_K5217004	+++++++	products not detected	pGAL1 is a promoter. It is added before the gene PgfB and followed by the PGK1 terminator.
BBa_K5217032	pGPM1_CbTPS1_PGK1t	composite	BBa_K5217000 BBa_K5217012 BBa_K5217004	+++++	plasmid constructed	pGPM1 is a promoter. It is added before the gene CbTPS1 and followed by the PGK1 terminator.
BBa_K5217033	pINO1_AmMYS_TEF1t	composite	BBa_K5217001 BBa_K5217008 BBa_K5217005	+++++	plasmid constructed	pINO1 is a promoter. It is added before the gene AmMYS and followed by the TEF1 terminator.

3. Parts Summary

In terms of technical progress, we have tested the synthetic biology production of seven terpenoids, including limonene, ocimene, cineole, nerolidol, nerol, borneol and myrcene. We successfully constructed expression vectors for all seven compounds. Four of these compounds (ocimene, cineole, nerolidol, and nerol) have been biosynthesized using yeast fermentation, with their production confirmed via Gas chromatography–mass spectrometry.