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Part

This year, we designed, built and characterized a set of parts. They were consisted of different parts collection and played different roles in different section of our parts. All the new parts had been submitted to The iGEM Parts Registry following the BioBricks assembly standard. On this page, we gave a brief description of our parts and we divided them into several sections according to the modules they worked in our project. For more detailed information about our parts, we highly recommend you to visit the corresponding parts page on The iGEM Parts Registry or turn to the Design and Engineering page of our wiki.

ZJU-China 2024 Parts(Basic)
module type designer code name length(bp) function and description
blocking module RNA Yufei Xie BBa_K5319669 HYER-J2/3 633 HYER with point mutation G338A
RNA BBa_K5319670 HYER-2nt 631 HYER with mutation 584_585del
RNA BBa_K5319671 HYER-cat 633 HYER with point mutation G567A
plasmid BBa_K5319672 lacUV5-J2/3-RS 3466 expressable G338A point mutated HYER with an extra 14-nt recruiting sequence
plasmid BBa_K5319673 lacUV5-2nt-RS 3464 expressable 584_585del mutated HYER with an extra 14-nt recruiting sequence
reporter BBa_K5319674 pJUMP42x-2a-TRS/RS-sfGFP 857 readout plasmid for HYER chracterization with the recognition site of TRS/RS
Yield improvement module RNA Yufei Xie BBa_K5319680 47CAG-2boxB 416 basic RNA scaffold with an restriction site between box B sequences, for enzyme co-localization
DNA Xuanhao Wang BBa_K5319716 vioE-Mu 583 This is a mutant of the ionin synthetase vioE, which is about 30% more catalytic than the wild type
DNA BBa_K5319675 λ peptide-GS linker-vioA 1347 vioA is one of a series of enzymes required for the synthesis of purple bacillin, and λ peption-GS linker can bind proteins with this junction to the RNA skeleton.
DNA BBa_K5319676 λ peptide-GS linker-vioB 2144 vioB is one of a series of enzymes required for the synthesis of purple bacillin, and λ peption-GS linker can bind proteins with this junction to the RNA skeleton.
DNA BBa_K5319677 λ peptide-GS linker-vioC 1460 vioC is one of a series of enzymes required for the synthesis of purple bacillin, and λ peption-GS linker can bind proteins with this junction to the RNA skeleton.
DNA BBa_K5319678 λ peptide-GS linker-vioD 1203 vioD is one of a series of enzymes required for the synthesis of purple bacillin, and λ peption-GS linker can bind proteins with this junction to the RNA skeleton.
communication module coding Liting Zhang BBa_K5319685 nahR-Q168R 903 This component is a specific receptor for salicylic acid, which also acts as a transcription factor for salicylic acid specific promoters
regulatory BBa_K5319686 Psal 155 This component is a salicylic acid-specific promoter, which is regulated by the salicylic acid-specific receptor nahR-Q168R(BBa_K5319685).
coding BBa_K5319703 pchBA 1733 It's a salicylic acid synthase derived from Pseudomonas aeruginosa.
coding BBa_K5319679 rhlR 726 This part is a recepter for C4-HSL and it regulates C4-HSL specific promoter.It can be used to chemically induce gene expression
coding BBa_K5319707 PagI 639 It is C4-HSL synthetase derived from Pantoea agglomerans pv. gypsophilae.
painting module protein-donmain Jiawei Wang BBa_K5319689 creN1-43 129 The N-terminal of a variant of the blue-induced photofission cre enzyme system, containing amino acids at positions 1-43, is predicted to have good low leakage levels and high polymerization activity
protein-donmain BBa_K5319691 creC44-285 636 The C-terminal of a variant of the blue-induced photofission cre enzyme system, containing amino acids at positions 44-285, is predicted to have good low leakage levels and high polymerization activity
protein-donmain BBa_K5319692 creN1-46 138 The N-terminal of a variant of the blue-induced photofission cre enzyme system, containing amino acids at positions 1-46, is predicted to have good low leakage levels and high polymerization activity
protein-donmain BBa_K5319693 creC47-285 587 The C-terminal of a variant of the blue-induced photofission cre enzyme system, containing amino acids at positions 47-285, is predicted to have good low leakage levels and high polymerization activity
protein-donmain BBa_K5319694 creN1-59 177 The N-terminal of a variant of the blue-induced photofission cre enzyme system, containing amino acids at positions 1-59, is predicted to have good low leakage levels and high polymerization activity
protein-donmain BBa_K5319717 creC60-285 548 The C-terminal of a variant of the blue-induced photofission cre enzyme system, containing amino acids at positions 60-285, is predicted to have good low leakage levels and high polymerization activity
scar Zhenyu Hu BBa_K5319696 loxp-tgg 34 A palindromic loxP mutant that has a palindromic sequence and has minor crosstalk with other different mutant
scar BBa_K5319697 loxp-gat 34 A palindromic loxP mutant that has a palindromic sequence and has minor crosstalk with other different mutant
scar BBa_K5319698 loxp-gcc 34 A palindromic loxP mutant that has a palindromic sequence and has minor crosstalk with other different mutant
ZJU-China 2024 Parts(Composite)
module type designer code name length(bp) function and description
communication module generator Liting Zhang BBa_K5319687 VioC expression circuit 1456 The device consists of a C4-HSL specific promoter, PRhlR(BBa_J61051), and a downstream VioC(BBa_K5319677). The C4-HSL binds to its specific receptor RhlR(BBa_C0171) and induces the specific promoter PRhlR(BBa_J61051)to express the downstream VioC, thus dye the cloth green. With C4-HSL, we can precisely control the coloring effect of the pigment on the fabric.
generator BBa_K5319688 VioE expression circuit 738 The device consists of a salicylic acid specific promoter Psal(BBa_K5319686) and a downstream VioE(BBa_K771203). Salicylic acid binds to its specific receptor nahR-Q168R (BBa_K5319685), inducing Psal to express downstream VioE, thus staining the cloth green. With salicylic acid, we can precisely control the coloring effect of the pigment on the fabric.
coding Jiawei Wang BBa_K5319715 RBS-creN-VVD-RBS-VVD-creC 6450 A new site of blue light induced DNA recombinase has relatively good activity