The first step of the engineering design cycle was recognition of a prominent problem which we could address. We identified a variety of potential problems to tackle, but ultimately decided on the global problem---Exocrine Pancreatic Insufficiency (EPI). Functionally, this causes an inadequate amount of lipolytic enzymes including pancreatic triacylglycerol lipase which is critical for the digestion of dietary fats. This indicated that the problem demanded a device which could be consumed and delivered to the small intestine within a short duration of consumption and release human pancreatic lipase and any necessary cofactors with causing any ill effects to an individual or the gut microbiome.
Research
Solution Development
Planning
Creation
Creation
Lipase (PNLIP)
Encodes the human lipase gene.
Procolipase (CLPS)
Encodes the human procolipase gene.
Switch Mechanism
Allows regulation of the device by glucose.
Ampicillin Resistance
Adds a drug resistance to confirm plasmid transformation.