MATERIALS

Materials

This page contains all of the materials that are not included in our registry of standard biological parts for the construction of synthetic operons that were used when developing biodegradable and environmentally sustainable adhesives with diverse applications in E. coli.

Strains

Bacterial strain	Used for
Caulobacter crescentus (Caulobacter vibroides) CB2 (DSM 4727, ATCC 15252)	Caulobacter crescentus CB2 polysaccharide tetrad assembly system and polysaccharide export apparatus protein biosynthesis
Caulobacter crescentus (Caulobacter vibroides) CB2A (DSM 25117)	Caulobacter crescentus CB2A polysaccharide tetrad assembly system and polysaccharide export apparatus protein biosynthesis
Hirschia baltica (DSM 5838, ATCC 49814)	Hirschia baltica HB polysaccharide tetrad assembly system and polysaccharide export apparatus protein biosynthesis
Escherichia coli Mach1	Fast plasmid propagation
Escherichia coli DH5α	Fast plasmid propagation
Escherichia coli dh10b	Multiplying pRED plasmid
Escherichia coli KRX (DE3)	Fast plasmid propagation
Escherichia coli BL21(DE3)	Expression of polysaccharide producing system from Caulobacter crescentus and Hirschia baltica, genome editing for more efficient polysaccharide production, investigation of polysaccharide composition.
Escherichia coli HMS174(DE3)	Expression of polysaccharide producing system from Caulobacter crescentus and Hirschia baltica, genome editing for more efficient polysaccharide production, investigation of polysaccharide composition.
Escherichia coli Rosetta(DE3)pLysS	Expression of polysaccharide producing system from Caulobacter crescentus and Hirschia baltica, genome editing for more efficient polysaccharide production, investigation of polysaccharide composition.
Escherichia coli C41(DE3)	Expression of polysaccharide producing system from Caulobacter crescentus and Hirschia baltica, genome editing for more efficient polysaccharide production, investigation of polysaccharide composition.
B3784 - WecA - Escherichia coli BW25113 ΔwecA	Donor DNA with kanamycin - resistance cassette and FRT sites for homology recombineering and knockout of wecA gene
B3784 - WecB - Escherichia coli BW25113 ΔwecB	Donor DNA with kanamycin - resistance cassette and FRT sites for homology recombineering and knockout of wecB gene
B3785 - WecF - Escherichia coli BW25113 ΔwecF	Donor DNA with kanamycin - resistance cassette and FRT sites for homology recombineering and knockout of wecF gene
B4481 - WecG - Escherichia coli BW25113 ΔwecG	Donor DNA with kanamycin - resistance cassette and FRT sites for homology recombineering and knockout of wecG gene
B3794 - WzzE - Escherichia coli BW25113 ΔwzzE	Donor DNA with kanamycin - resistance cassette and FRT sites for homology recombineering and knockout of wzzE gene

Plasmids

Plasmid	Characteristics	Used for	Source
pRSF-Duet-1	ori RSF1030, lacI, T7/lac promoter, KanR	The backbone of holdfast tetrad assembly operon	VU LSC Institute of Biochemistry
pACYC-Duet-1	ori p15A, lacI, T7/lac promoter, CmR	The backbone of holdfast polymerization and export apparatus	VU LSC Institute of Biochemistry
pRED	araC, pBAD, tetR, recs, Ori pSC101	Plasmid producing lambda Red proteins for homology recombineering	VU LSC Institute of Biotechnology
p708	ori pSC101, repA, Cl578, tetR	Plasmid producing flipase for homology recombineering	VU LSC Institute of Biotechnology
pTargetF	Bacterial Expression, gRNA	Constitutive expression of sgRNA without donor editing template DNA	VU LSC Institute of Biochemistry
pCas	Bacterial Expression, CRISPR ; Lambda Red recombinase	Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA	VU LSC Institute of Biochemistry

Golden Gate Assembly primers

Primer name	Sequence (5’ → 3’)	Used for
F_1MCS_HB_pACYCDuet_AarI	atacacctgcccatctaatcgaacagaaagtaatcgtattg	Linearisation of Hirschia baltica 1 multiple cloning sequence in pACYCDuet vector
R_1MCS_HB_pACYCDuet_AarI	atacacctgcccatggtatatctccttattaaagttaaac	Linearisation of Hirschia baltica 1 multiple cloning sequence in pACYCDuet vector
F_2MCS_HB_pACYCDuet_AarI	atacacctgcccatctgttctagcataaccccttgg	Linearisation of Hirschia baltica 2 multiple cloning sequence in pACYCDuet vector
R_2MCS_HB_pACYCDuet_AarI	atacacctgcccattcatatatctccttcttatacttaactaatatac	Linearisation of Hirschia baltica 2 multiple cloning sequence in pACYCDuet vector
F_1MCS_CB2_pACYCDuet_AarI	atacacctgcccatgtggcgaacagaaagtaatcgtattg	Linearisation of Caulobacter crescentus CB2 1 multiple cloning sequence in pACYCDuet vector
R_1MCS_CB2_pACYCDuet_AarI	atacacctgcccatggtatatctccttattaaagttaaac	Linearisation of Caulobacter crescentus CB2 1 multiple cloning sequence in pACYCDuet vector
F_2MCS_CB2_pACYCDuet_AarI	atacacctgcccatctgccttaattaacctaggctgctg	Linearisation of Caulobacter crescentus CB2 2 multiple cloning sequence in pACYCDuet vector
R_2MCS_CB2_pACYCDuet_AarI	atacacctgcccatatgtatatctccttcttatacttaact	Linearisation of Caulobacter crescentus CB2 2 multiple cloning sequence in pACYCDuet vector
F_1MCS_CB2A_pACYCDuet_AarI	atacacctgcccattagatcgaacagaaagtaatcgtattg	Linearisation of Caulobacter crescentus CB2A 1 multiple cloning sequence in pACYCDuet vector
R_1MCS_CB2A_pACYCDuet_AarI	atacacctgcccatggtatatctccttattaaagttaaac	Linearisation of Caulobacter crescentus CB2A 1 multiple cloning sequence in pACYCDuet vector
F_2MCS_CB2A_pACYCDuet_AarI	atacacctgcccatcttaattaacctaggctgctg	Linearisation of Caulobacter crescentus CB2A 2 multiple cloning sequence in pACYCDuet vector
R_2MCS_CB2A_pACYCDuet_AarI	atacacctgcccatatgtatatctccttcttatacttaact	Linearisation of Caulobacter crescentus CB2A 2 multiple cloning sequence in pACYCDuet vector
F_CB2_pRSF_1MCS_AarI	atacacctgcccattcgcgcataatgcttaagtcgaac	Linearisation of Caulobacter crescentus CB2 1 multiple cloning sequence in pRSFDuet vector
F_HB_pRSF_1MCS_AarI	atacacctgcccatatgagcataatgcttaagtcgaacag	Linearisation of Hirschia baltica 1 multiple cloning sequence in pRSFDuet vector
F_HB_pRSF_2MCS_Aari	atacacctgcccatctgaagcttaattaacctaggctg	Linearisation of Hirschia baltica 2 multiple cloning sequence in pRSFDuet vector
F_CB2A_pRSF_1MCS_AarI	atacacctgcccatgcatcataatgcttaagtcgaacag	Linearisation of Caulobacter crescentus CB2A 1 multiple cloning sequence in pACYCDuet vector
F_CB2A_pRSF_2MCS_AarI	atacacctgcccatctgccgcagcttaattaacctag	Linearisation of Caulobacter crescentus CB2A 2 multiple cloning sequence in pACYCDuet vector
F_1MCS_HB_hfsA_AarI	atacacctgcccattaccatgatgcaagacaatcaagccac	PCR of Hirschia baltica HfsA
R_1MCS_HB_hfsA_AarI	atacacctgcccatacaagatttattgcttcgatgtcacaccaatg	PCR of Hirschia baltica HfsA
F_HB_hfsB_optimised	atacacctgcccatttgtaaggagatataccatgaaagatttacgcaaagatttgaccg	PCR of Hirschia baltica HfsB
R_HB_hfsB_optimised	atacacctgcccatctgtgatttatccagagatttcatcggccaaac	PCR of Hirschia baltica HfsB
F_1MCS_HB_hfsD_AarI	atacacctgcccatacagaaggagatataccatgatgaaaaacaacgcatccatcc	PCR of Hirschia baltica HfsD
R_1MCS_HB_hfsD_AarI	atacacctgcccatttagaaggcgttgtcttttaggttg	PCR of Hirschia baltica HfsD
F_2MCS_HB_hfsC_AarI	atacacctgcccataaggagatatacatatgttcaacaacgacacgtgc	PCR of Hirschia baltica HfsC
R_2MCS_HB_hfsC_AarI	atacacctgcccatcacgaatttagctgcgtttcttcgtagtac	PCR of Hirschia baltica HfsC
F_2MCS_HB_hfsI_AarI	atacacctgcccatcgtgaaggagatatacatatgatattgagcccattggcaaacg	PCR of Hirschia baltica HfsI
R_2MCS_HB_hfsI_AarI	atacacctgcccatacagtcaattttttgcgtacgggctag	PCR of Hirschia baltica HfsI
F_1MCS_HB_hfsE_AarI	atacacctgcccattaccatgtttaaatttgtaatggaaatcgc	PCR of Hirschia baltica HfsE
R_1MCS_HB_hfsE_AarI	atacacctgcccatgaattacctagcgctgtgtttttgag	PCR of Hirschia baltica HfsE
F_1MCS_HB_hfsG_AarI	atacacctgcccatattcaaggagatatacatatgaacacaacgccccaac	PCR of Hirschia baltica HfsG
R_1MCS_HB_hfsG_AarI	atacacctgcccatttcgtaatcaatcatgaagagcctcc	PCR of Hirschia baltica HfsG
F_1MCS_HB_hfsH_AarI	atacacctgcccatcgaaaaggagatatacatatgattgattggcattacac	PCR of Hirschia baltica HfsH
R_1MCS_HB_hfsH_AarI	atacacctgcccattcatgagattaggccgtag	PCR of Hirschia baltica HfsH
F_2MCS_HB_hfsJ_AarI	atacacctgcccatacatatgagccgcgcttgc	PCR of Hirschia baltica HfsJ
R_2MCS_HB_hfsJ_AarI	atacacctgcccatagtatctctaccttttacgttgtatcaatgaaaatag	PCR of Hirschia baltica HfsJ
F_2MCS_HB_hfsL_AarI_II	atacacctgcccattactaaggagatataatgaaccaatccgtaaaagtcagtattg	PCR of Hirschia baltica HfsL
R_2MCS_HB_hfsL_AarI_II	atacacctgcccatccttatgttaagttgcgcttttgataacttttttgc	PCR of Hirschia baltica HfsL
F_2MCS_HB_hfsK_AarI_II	atacacctgcccataaggaaggagatataatgtggtttgatgtaggaccgttag	PCR of Hirschia baltica HfsK
R_2MCS_HB_hfsK_AarI_II	atacacctgcccattcagttatgcgtttgaattgtgcatacc	PCR of Hirschia baltica HfsK
F_1MCS_CB2_hfsA_AarI	atacacctgcccattaccatgtcgacgagcgcct	PCR of Caulobacter crescentus CB2 HfsA
R_1MCS_CB2A_hfsA_MUT_AarI	atacacctgcccatacctgctgggcgtagg	PCR of Caulobacter crescentus CB2 HfsA
F_1MCS_CB2A_hfsA_MUT_AarI	atacacctgcccataggttaccgacgtgaattcacg	PCR of Caulobacter crescentus CB2 HfsA
R_1MCS_CB2_hfsA_AarI	atacacctgcccattaagacttcaccgcttgacggc	PCR of Caulobacter crescentus CB2 HfsA
F_1MCS_CB2_hfsB_AarI	atacacctgcccatcttaaaggagatataccatggtggatttgaacgtcgaga	PCR of Caulobacter crescentus CB2 HfsB
R_1MCS_CB2_hfsB_AarI	atacacctgcccattcaatactcacggcaggatggcct	PCR of Caulobacter crescentus CB2 HfsB
F_1MCS_CB2_hfsD_AarI	atacacctgcccatttgaaaggagatataccatgcagagccgcacgca	PCR of Caulobacter crescentus CB2 HfsD
R_1MCS_CB2_hfsD_AarI	atacacctgcccatccacttagcggatgttgccgttgatc	PCR of Caulobacter crescentus CB2 HfsD
F_2MCS_CB2_HfsF_AarI	atacacctgcccatacatatgttctggcgcggcgtcct	PCR of Caulobacter crescentus CB2 HfsF
R_2MCS_CB2_hfsF_AarI	atacacctgcccatccttctatcatgcggcttgcgcctttc	PCR of Caulobacter crescentus CB2 HfsF
F_2MCS_CB2_hfsC_AarI	atacacctgcccataaggagatatacatatgacctacgcgccggcgcccct	PCR of Caulobacter crescentus CB2 HfsC
R_2MCS_CB2_hfsC_AarI	atacacctgcccatctcagatctagacttcctcctgagcccggtcggag	PCR of Caulobacter crescentus CB2 HfsC
F_2MCS_CB2_hfsI_AarI	atacacctgcccattgagaaggagatatacatatgacgacgccctatcgccac	PCR of Caulobacter crescentus CB2 HfsI
R_2MCS_CB2_hfsI_AarI	atacacctgcccatgcagttagctctgggcgcgct	PCR of Caulobacter crescentus CB2 HfsI
F_1MCS_CB2_hfsE_AarI	atacacctgcccattaccatggttttcccaacgacgagcatc	PCR of Caulobacter crescentus CB2 HfsE
R_1MCS_CB2_hfsE_AarI	atacacctgcccatacaggatctagcgcacggcggac	PCR of Caulobacter crescentus CB2 HfsE
F_1MCS_CB2_hfsJ_AarI	atacacctgcccatctgtaaggagatataccatgtctccaagactaggagactc	PCR of Caulobacter crescentus CB2 HfsJ
R_1MCS_CB2_hfsJ_AarI	atacacctgcccatcatctagttagcgcgccgcctc	PCR of Caulobacter crescentus CB2 HfsJ
F_1MCS_CB2_hfsG_AarI	atacacctgcccatgatgaaggagatataccatgaacgcgcccgtc	PCR of Caulobacter crescentus CB2 HfsG
R_1MCS_CB2_hfsG_AarI	atacacctgcccatgcgatcagacggcctcgctg	PCR of Caulobacter crescentus CB2 HfsG
F_2MCS_CB2_hfsL_AarI	atacacctgcccatacatatggcgcgcgtcacg	PCR of Caulobacter crescentus CB2 HfsL
R_2MCS_CB2_hfsL_AarI	atacacctgcccatccttactagattcacgcggtcctcccgtag	PCR of Caulobacter crescentus CB2 HfsL
F_2MCS_CB2_hfsH_AarI	atacacctgcccataaggagatatacatatgcaggtagaattcgag	PCR of Caulobacter crescentus CB2 HfsH
R_2MCS_CB2_hfsH_AarI	atacacctgcccatcatcagactagagcccgatccg	PCR of Caulobacter crescentus CB2 HfsH
F_2MCS_CB2_hfsK_AarI	atacacctgcccatgatgaaggagatatacatatgcccatcgaaatcgtcaaagcgctg	PCR of Caulobacter crescentus CB2 HfsK
R_2MCS_CB2_hfsK_AarI	atacacctgcccattcagtgcagtccgcgcagc	PCR of Caulobacter crescentus CB2 HfsK
F_1MCS_CB2A_hfsA_AarI	atacacctgcccattaccatgtcgacgagcgcctg	PCR of Caulobacter crescentus CB2A HfsA
R_1MCS_CB2A_hfsA_STOP_AarI	atacacctgcccatcaagtcaccgcttgacggc	PCR of Caulobacter crescentus CB2A HfsA
R_1MCS_CB2_for_CB2A_hfsB_AarI	atacacctgcccattgacatcacggcaggatggcct	PCR of Caulobacter crescentus CB2A HfsA, used for HfsB insertion in CB2A plasmid
F_1MCS__CB2_for_CB2A_hfsB_AarI_II	atacacctgcccatcttgtgtaaggagatataccatggtggatttgaacgtcgag	PCR of Caulobacter crescentus CB2A HfsA, used for HfsB insertion in CB2A plasmid
F_1MCS_CB2A_hfsD_AarI	atacacctgcccatgtcataaaggagatataccatgcagagccgcacg	PCR of Caulobacter crescentus CB2A HfsK
R_1MCS_CB2A_hfsD_AarI	atacacctgcccattctattagcggatgttgccgttg	PCR of Caulobacter crescentus CB2A HfsK
F_1MCS_CB2A_hfsE_AarI	atacacctgcccattaccatggttttcccaacgacgagcatc	PCR of Caulobacter crescentus CB2A HfsE
R_1MCS_CB2A_hfsE_AarI	atacacctgcccatatcactagcgcacggcggac	PCR of Caulobacter crescentus CB2A HfsE
F_1MCS_CB2A_hfsJ_AarI	atacacctgcccattgatttaaaggagatataccatgtctccaagactaggagactctg	PCR of Caulobacter crescentus CB2A HfsJ
R_1MCS_CB2A_hfsJ_AarI	atacacctgcccatgactttagcgcgccgcctc	PCR of Caulobacter crescentus CB2A HfsJ
F_1MCS_CB2A_hfsG_AarI	atacacctgcccatagtcttaaaggagatataccatgaacgcgcccgtcac	PCR of Caulobacter crescentus CB2A HfsG
R_1MCS_CB2A_hfsG_AarI	atacacctgcccatatgctcagacggcctcgctgtag	PCR of Caulobacter crescentus CB2A HfsG
F_2MCS_CB2A_hfsL_AarI	atacacctgcccatacatatggcgcgcgtcacggtc	PCR of Caulobacter crescentus CB2A HfsL
R_2MCS_CB2A_hfsL_AarI	atacacctgcccatccttatgctattcacgcggtcctcccgtagaattg	PCR of Caulobacter crescentus CB2A HfsL
F_2MCS_CB2A_hfsH_AarI	atacacctgcccataaggagatatacatatgcaagtggaattcgagaaggtcgac	PCR of Caulobacter crescentus CB2A HfsH
R_2MCS_CB2A_hfsH_AarI	atacacctgcccatgtcgctagagcccgatccgccgcgag	PCR of Caulobacter crescentus CB2A HfsH
F_2MCS_CB2A_hfsK_AarI	atacacctgcccatcgacttaaaggagatatacatatgcccatcgaaatcgtcaaagcgctg	PCR of Caulobacter crescentus CB2A HfsK
R_2MCS_CB2A_hfsK_AarI	atacacctgcccatgcagtcagtgcagtccgcgcag	PCR of Caulobacter crescentus CB2A HfsK
F_2MCS_CB2A_HfsF_AarI	atacacctgcccatacatatgttctggcgcggcgt	PCR of Caulobacter crescentus CB2A HfsF
R_2MCS_CB2A_HfsF_AarI	atacacctgcccatccttttatagttcatgcggcttgcgcct	PCR of Caulobacter crescentus CB2A HfsF
F_2MCS_CB2A_HfsC_AarI	atacacctgcccataaggagatatacatatgacctacgcgccggcg	PCR of Caulobacter crescentus CB2A HfsC
R_2MCS_CB2A_HfsC_AarI	atacacctgcccatacagagtctagacttcctcctgagcccggt	PCR of Caulobacter crescentus CB2A HfsC
F_2MCS_CB2A_HfsI_AarI	atacacctgcccatctgtaaggagatatacatatgacgacgccctatcgcca	PCR of Caulobacter crescentus CB2A HfsI
R_2MCS_CB2A_HfsI_AarI	atacacctgctaattaagttagctctgggcgcgct	PCR of Caulobacter crescentus CB2A HfsI
F_HB_pACYCDuet_MCS1_cPCR	ctcaatgcaaccaaagcagatgc	Colony PCR of Hirschia baltica
R_pACYCDuet_MCS1_cPCR	gattatgcggccgtgtacaatacg	Colony PCR of Hirschia baltica
F_HB_pACYCDuet_MCS2_cPCR	ctaccaccgcgtactacgaag	Colony PCR of Hirschia baltica
R_pACYCDuet_MCS2_cPCR	gaccgtgtgcttctcaaatgc	Colony PCR of Hirschia baltica
F_CB2A_pACYCDuet_MCS1_cPCR	ctccgaccgggctcagg	Colony PCR of Caulobacter crescentus CB2A
F_CB2_pACYCDuet_MCS2_cPCR	ctgggtgctgttcgtctcg	Colony PCR of Caulobacter crescentus CB2
F_HB_pETDuet_MCS2_qPCR_II	gaggactaggaaaacccctc	Colony PCR of Hirschia baltica
F_CB2_pACYC_ori_AmpR_AarI	atacacctgcccatattctgtagcacctgaagtcagc	AmpR/ori switch primers
R_CB2_pACYC_AmpR_AarI	atacacctgcccatacgccagttattggtgcccttaaac	AmpR/ori switch primers
F_AmpR_AarI_pACYC	atacacctgcccatgcgtttctacaaactcttttg	AmpR/ori switch primers
R_ori_AmpR_pACYC_AarI	atacacctgcccatgaatgccaggaaccgtaaaaagg	AmpR/ori switch primers
F_pRSF_AmpR_AarI	atacacctgcccattaggcatgcagcgctctt	AmpR/ori switch primers
R_pRSF_AmpR_AarI	atacacctgcccatctgcagtgccacttgcggagac	AmpR/ori switch primers
F_AmpR_AarI	atacacctgcccatgcaggattatcaaaaaggatcttcacct	AmpR/ori switch primers
R_AmpR_AarI	atacacctgcccatcctagcgtttctacaaactcttttg	AmpR/ori switch primers
F_pRSF_linear_Nterm_AarI_purif	atacacctgcctatgatgatgatgcatggtatatctccttattaaagttaaac	Linearisation of N terminus His-tagged genes
R_pRSF_linear_Nterm_AarI_purif	atacacctgcccatgacttgcttaagtcgaacag	Linearisation of N terminus His-tagged genes
F_pRSF_linear_Cterm_AarI_purif	atacacctgcccatggtatatctccttattaaagttaaac	Linearisation of C terminus His-tagged genes
R_pRSF_linear_Cterm_AarI_purif	atacacctgcccatcatcatcattaaagctagcttgcttaagtcg	Linearisation of C terminus His-tagged genes
F_CB2_HfsG_AarI_purif	atacacctgcccatcatcatcatcatggttcttctggtaacgcgcccgtcactgatc	PCR for purifying Caulobacter crescentus CB2 HfsG
R_CB2_HfsG_AarI_purif	atacacctgcccatagtctcagacggcctcgctgtag	PCR for purifying Caulobacter crescentus CB2 HfsG
F_CB2_HfsJ_AarI_purif	atacacctgcccattaccatgtctccaagactaggagactctgc	PCR for purifying Caulobacter crescentus CB2 HfsJ
R_CB2_HfsJ_AarI_purif	atacacctgccactgatgatgatgatgaccagaagaaccgcgcgccgcctcgttg	PCR for purifying Caulobacter crescentus CB2 HfsJ
F_CB2_HfsL_AarI_purif	atacacctgcccatcatcatcatcatggttcttctggtgcgcgcgtcacggtcatg	PCR for purifying Caulobacter crescentus CB2 HfsL
R_CB2_hfsL_AarI_purif	atacacctgcccatagtctcacgcggtcctcccgtag	PCR for purifying Caulobacter crescentus CB2 HfsL
F_CB2_HfsH_AarI_purif	atacacctgcccattaccatgcaggtagaattcgagaaggtcgacg	PCR for purifying Caulobacter crescentus CB2 HfsH
R_CB2_HfsH_AarI_purif	atacacctgccactgatgatgatgatgaccagaagaaccgagcccgatccgccgcgag	PCR for purifying Caulobacter crescentus CB2 HfsH
F_CB_HfsK_AarI_purif	atacacctgcccatcatcatcatcatggttcttctggtcccatcgaaatcgtcaaagc	PCR for purifying Caulobacter crescentus CB2 HfsK
R_CB_HfsK_AarI_purif	atacacctgcccatagtctcagtgcagtccgcgca	PCR for purifying Caulobacter crescentus CB2 HfsK
F_CB2A_HfsH_AarI_purif	atacacctgcccattaccatgcaagtggaattcgagaaggtcgac	PCR for purifying Caulobacter crescentus CB2 HfsH
F_HB_HfsG_AarI_purif	atacacctgcccattaccatgaacacaacgccccaac	PCR for purifying Hirschia baltica HfsG
R_HB_hfsG_AarI_purif	atacacctgccactgatgatgatgatgaccagaagaaccatcatgaagagcctccgca	PCR for purifying Hirschia baltica HfsG
F_HB_HfsH_AarI_purif	atacacctgcccattaccatgattgattggcattacacacc	PCR for purifying Hirschia baltica HfsH
R_HB_HfsH_AarI_purif	atacacctgccactgatgatgatgatgaccagaagaacctgagattaggccgtagcttc	PCR for purifying Hirschia baltica HfsH
F_HB_HfsK_AarI_purif	atacacctgcccatcatcatcatcatggttcttctggttggtttgatgtaggaccgt	PCR for purifying Hirschia baltica HfsK
R_HB_HfsK_AarI_purif	atacacctgcccatagtcagctttatgcgtttgaattgtgcatac	PCR for purifying Hirschia baltica HfsK
F_HB_HfsL_AarI_purif	atacacctgcccatcatcatcatcatggttcttctggtaaccaatccgtaaaagtcag	PCR for purifying Hirschia baltica HfsL
R_HB_hfsL_AarI_purif	atacacctgcccatagtcttaagttgcgcttttgataac	PCR for purifying Hirschia baltica HfsL
F_HB_HfsJ_AarI_purif	atacacctgcccattaccatgagccgcgcttgc	PCR for purifying Hirschia baltica HfsJ
R_HB_HfsJ_AarI_purif	atacacctgccactgatgaccagaagaaccccttttacgttgtatcaatgaaaatag	PCR for purifying Hirschia baltica HfsJ
F_pRSF_purif_cPCR	gtgagcggataacaattcccctgtag	Colony PCR for purifying Caulobacter crescentus proteins
F_gDNR_WecA	aaactgtttctgttttttgcccgtag	gDNA fragments for gene KO
R_gDNR_WecA	aaaactacgggcaaaaaacagaaaca	gDNA fragments for gene KO
F_gDNR_WecA_new	ctcggaccgttgttttagagctagaaatagcaag	Primers for introducing gRNA templates to pTargetF plasmid
R_gDNR_WecA_new	caccatctccctagtattatacctaggactgagctagc	Primers for introducing gRNA templates to pTargetF plasmid
F_gDNR_WecF	aaaccaccataaccgaaccgttttgg	gDNA fragments for gene KO
R_gDNR_WecF	aaaaccaaaacggttcggttatggtg	gDNA fragments for gene KO
F_dDNR_WecA	tttactgacagtgagtactgatctcatcagtatttttttattcacgacactagatagcatagacgtagata	dDNA fragments for homology arms in gene KO
R_dDNR_WecA	acgtttgcggaagtttggtttatccactaaaccgactttttttgccaccttatctacgtctatgctatcta	dDNA fragments for homology arms in gene KO
F_dDNR_WecF	cgtaggcgggcatgactgtactgattcacgtactgggatcggatatcccttagatagcatagacgtagata	dDNA fragments for homology arms in gene KO
R_dDNR_WecF	aactcgcgcgcgtgctcgctcgtcgcggccagcgcgtcattgaaaaaccgtatctacgtctatgctatcta	dDNA fragments for homology arms in gene KO
F_dDNR_WecG	gttgaaggataacaatgaataacaacaccacggcaccaacctatacgctgtagatagcatagacgtagata	dDNA fragments for homology arms in gene KO
R_dDNR_WecG	agctgcccgtcagcaaacagataatcgagggcgtgctgcatatcacgccatatctacgtctatgctatcta	dDNA fragments for homology arms in gene KO
R_100bp_del_WecA	agagagatgcatgcggaatatagtaatcgacaattccgaacgtgaagcaatatctacgtctatgctatcta	Oligonucleotides for creating dDNA - homology arms and introducing 100bp deletion
R_100bp_del_WecF	cgcgatgaccgcttccgccagcgattttttcccagggaaaaattgcacagtatctacgtctatgctatcta	Oligonucleotides for creating dDNA - homology arms and introducing 100bp deletion
F_gDNR_pTargetF_WecA	ttttgcccgtagttttagagctagaaatagcaag	Primers for introducing gRNA templates to pTargetF plasmid
R_gDNR_pTargetF_WecA	aacagaaacactagtattatacctaggactgagctagc	Primers for introducing gRNA templates to pTargetF plasmid
F_gDNR_pTargetF_WecF	gaaccgttttggttttagagctagaaatagcaag	Primers for introducing gRNA templates to pTargetF plasmid
R_gDNR_pTargetF_WecF	ggttatggtgctagtattatacctaggactgagctagc	Primers for introducing gRNA templates to pTargetF plasmid
F_gDNR_pTargetF_WecG	agttgattggtgttttagagctagaaatagcaag	Primers for introducing gRNA templates to pTargetF plasmid
R_gDNR_pTargetF_WecG	gtaagccacgctagtattatacctaggactgagctagc	Primers for introducing gRNA templates to pTargetF plasmid
F_dDNR_WecA	ctcggaccgttgttttagagctagaaatagcaagtagatagcatagacgtagata	Sticky hands for dDNR templates
R_dDNR_WecA	tgaacgcattaatggccgcccagacggcaaatagcgtcaggaagtaaccatatctacgtctatgctatcta	Sticky hands for dDNR templates
F_check_WecA	gcgcgctggatgaccgttttg	Primers for checking if gene knock-out happened
R_check_WecA	ctgcccgcatcacccataaagactttg	Primers for checking if gene knock-out happened
F_dDNR_WecG	attgaaggataacaatgaataacaccaccacggcaccgacctatacactgtagatagcatagacgtagata	Primers for checking if gene knock-out happened
R_check_WecG	ctgcgggtactttttacgtactgaac	Primers for checking if gene knock-out happened
F_dDNR_WzyE	cagcgggcgttggcgattgccgccggggaggtcgcatgagtctgctgcaatagatagcatagacgtagata	Primers for checking if gene knock-out happened
F_check_WzyE	catttgccggtgctgtttactac	Primers for checking if gene knock-out happened
R_check_WzzE	tgaacggacatccaggttacg	Primers for checking if gene knock-out happened
F_WecB_homology	cagacctatcgctatttgc	Primers for checking if gene knock-out happened
R_WecB_homology	gtgggtacagcaatcagc	Primers for checking if gene knock-out happened