| Description | Part number | Function |
|---|---|---|
| LCI-M3 | BBa_K5487124 | The encoded anchoring peptide specifically recognizes plastic PP (polypropylene). |
| TA2-M2 | BBa_K5487100 | The encoded anchoring peptide specifically binds to plastic PS (polystyrene). |
| Cg-Def-v2 S19K/K10L/N13H | BBa_K5487102 | The encoded anchoring peptide specifically recognizes plastic PLA (polylactic acid). |
| S-COMT | BBa_K5487007 | The primary function of S-COMT is the methylation of catechols, which is a key step in the biosynthesis of vanillin from protocatechuic aldehyde. |
| Sfp | BBa_K5487008 | Encode phosphopanthenylmercaptoethaminotransferase. |
| Z1-PETase | BBa_K5487001 | Z1-PETase is a modified enzyme from a bacteria that breaks down PET plastic better. It works faster, lasts longer, and can work at lower temperatures. This makes it useful for recycling PET and helping the environment. |
| Lpp-OmpA | BBa_K5487002 | The Lpp-OmpA fusion protein helps put different peptides and proteins on the outside of bacteria like E. coli. The Lpp part guides the protein to the right place and helps it get out, while OmpA keeps it stable and shows it a lot. |
| yahK-sgRNA Box | BBa_K5487104 | The gene sequence cassette utilized for the introduction of the sgRNA to the target gene(YahK). |
| AmpR promoter | BBa_K5487112 | A promoter responsible for initiating the transcription of the ampicillin resistance gene. |
| yahK-HA1/2 | BBa_K5487107 | The homology arms of YahK gene. |
| Rep101-pSC101 ori-rrnB T1 terminator Backbone | BBa_K5487113 | Plasmid deletion switch, containing a low-copy temperature-sensitive promoter that prevents replication at temperatures exceeding 30 degrees Celsius. |
| RBS | BBa_K5487117 | efficient ribosome binding site from bacteriophage T7 gene 10. |
| TEV site | BBa_K5487118 | tobacco etch virus (TEV) protease recognition and cleavage site. |
| 6xHis | BBa_K5487119 | 6xHis affinity tag. |
| Linker | BBa_K5487120 | A simple piece of protein that links anchor peptides and GFP together. |
| MCS Box-lac operator-lac promoter-CAP binding site | BBa_K5487114 | Part of plasmid backbone. |
| araBAD promoter | BBa_K5487115 | Part of the arabinose operon, which includes three genes: araB, araA, and araD, encoding enzymes essential for arabinose metabolism and controlling the expression of these genes, which are activated in the presence of arabinose. |
| Description | Part number | Function |
|---|---|---|
| TA2-M2-HIS-TEV-GFP | BBa_K5487122 | The TA2 anchoring peptide was synthesized as the vector skeleton of the fusion protein. |
| Cg-Def-HIS-TEV-GFP | BBa_K5487123 | The CG-Def anchoring peptide was synthesized as the vector skeleton of the fusion protein. |
| LCI-M3-HIS-TEV-GFP | BBa_K5487125 | THE LCI anchoring peptide was synthesized as the vector skeleton of the fusion protein. |
| Lac Promoter+Sfp+Term | BBa_K5487010 | The pSfp plasmid is a genetic element designed to carry and express the sfp gene, which encodes the surfactant protein A (SP-A) phosphopantetheinyl transferase. |
| T7 Promoter + lac operator + RBS + Lpp-OmpA + sfGFP | BBa_K5487005 | The composite part, consisting of the T7 Promoter, lac operator, RBS, Lpp-OmpA, and sfGFP, is designed for the controlled expression and membrane targeting of superfolder green fluorescent protein (sfGFP) in bacterial systems. |
| T7 Promoter+lac operator+RBS+TphA2+RBS+TphA1+Term+T7 promoter+lac operator+RBS+TphA3+RBS+TphB2+Term | BBa_K5487004 | Carry and express the enzymes required for the conversion of terephthalic acid (TPA) into protocatechuic acid (PCA). |
| Enhanced Surface Display of Z1-PETase and MHETase Fusion Protein | BBa_K5487003 | This Lpp-OmpA-based composite part is engineered for efficient surface presentation of the Z1-PETase and MHETase fusion protein on bacterial cells. |
| Lac Promoter+NiCAR+Lac promoter+S-COMT+Term | BBa_K5487101 | The plasmid is designed to express a set of enzymes crucial for the biosynthesis of vanillin from terephthalic acid (TPA). |
| PHCY-yahK-KO | BBa_K5487110 | The plasmid is designed to knockout YahK gene in E.coli BL21(DE3) genome. |
| Description | Part number | Function |
|---|---|---|
| sfGFP | BBa_I746916 | Superfolder GFP (sfGFP) is an enhanced variant of GFP with improved folding efficiency and stability. |
| MHETase | BBa_K3997001 | MHETase can break down MHET into TPA. |
| AmpR | BBa_K3766012 | An E. coli gene coding for a β-lactamase enzyme. |
| TphA1 | BBa_K808011 | TphA1 forms together with TphA2 and TphA3 the terephthalic acid 1,2-dioxygenase system (TERDOS). |
| TphA2 | BBa_K4701222 | TPA 1,2-dioxygenase oxydase subunit alpha. |
| TphA3 | BBa_K4701223 | TPA 1,2-dioxygenase (TPADO) subunit beta (UniProt: Q3C1D4) encoded by the tphA3II gene, is one of the three proteins that form the TPADO complex. |
| TphB | BBa_K2013010 | TphB codes 1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase(DCDDH), which can hydrolyze 1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate to protocatechuate. |
| NiCAR | BBa_K4701223 | TphB codes 1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase(DCDDH), which can hydrolyze 1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate to protocatechuate. |