Stage1: Strain Culture(7.1-7.30)
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OD750 Measurement
Table 1: Growth curve of cyanobacteria Cultured under room temperature and natural light conditions.
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OD750 Measurement
Table 2: Growth curve of cyanobacteria Cultured in a light incubator.
Stage2: Strain Transformation(7.17-8.29)
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DH5α amplification of cscB gene and cscB plasmid extraction.
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Stage3: Expression Verification(9.7-9.22)
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We measured sucrose content from September 7 to 22, showing that transformed cyanobacteria had decreased intracellular sucrose and increased supernatant sucrose, indicating their ability to secrete sucrose.
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