In addition to the overall scheme, a more detailed timetable for the wet lab stage of the project can be found below.
05 August - 11 August 2024
TSS Competent Cell Growing |
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12 - 18 August 2024
Glycerol Stock Preparation |
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19 - 25 August 2024
Transforming DH5alpha cells with an antibiotic resistance plasmid to test electroporation |
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26 August - 1 September 2024
Gibson assembly of the OneRing plasmid |
02 - 08 September 2024
Heat Shock of the plasmid to E. coli & mini-prep of the plasmid |
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09 - 15 September 2024
PCR,Gel Extraction, and Restriction Enzyme Digestion to Amplify the Sequences of the Plasmid |
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16 - 22 September 2024
Conjugation Efficiency Assay & Preparing the Killing Assay |
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23 - 29 September 2024
Data Analysis of the Results and Completion of the Wet Lab Stage |