Overall Scheme

Wet Lab Detailed Timetable

In addition to the overall scheme, a more detailed timetable for the wet lab stage of the project can be found below.

05 August - 11 August 2024

TSS Competent Cell Growing

» 12 - 18 August 2024

Glycerol Stock Preparation

» 19 - 25 August 2024

Transforming DH5alpha cells with an antibiotic resistance plasmid to test electroporation

» 26 August - 1 September 2024

Gibson assembly of the OneRing plasmid


02 - 08 September 2024

Heat Shock of the plasmid to E. coli & mini-prep of the plasmid

» 09 - 15 September 2024

PCR,Gel Extraction, and Restriction Enzyme Digestion to Amplify the Sequences of the Plasmid

» 16 - 22 September 2024

Conjugation Efficiency Assay & Preparing the Killing Assay

» 23 - 29 September 2024

Data Analysis of the Results and Completion of the Wet Lab Stage

Image Gallery of the Experiments