In 2024, OUC-Haide iGEM team focuses on utilizing Aureobasidium melanogenum BZ-11 to optimize β-glucan production through synthetic biology. Our project demonstrates two aspects of optimizing metabolic pathways to reduce byproduct accumulation and expressing hemoglobin to enhance oxygen uptake for improved β-glucan production. It is divided into two parts. (Table 1)
First, we target two genes responsible for unwanted products PKS (linked to melanin) and AGS2 (linked to pullulan). By knocking out these genes, we reduce the presence of these impurities, thereby increasing the yield of β-glucan, which holds significant industrial value.
Second, we introduce three cell wall anchoring sequences, AM.Sed, AM.Tip, and AM.CWP, each connected to EGFP and VHb, aiming to explore the efficiency of these anchoring proteins. Finally, we select the optimal protein to enhance the oxygen uptake capacity of chassis organisms, improving the efficiency of the entire system.
| Part ID | Part Type | Description |
|---|---|---|
| BBa | Coding | PKS |
| BBa | Coding | AGS2 |
| BBa | Plasmid Backbone | AM.CWP + EGFP |
| BBa | Plasmid Backbone | AM.Sed + EGFP |
| BBa | Plasmid Backbone | AM.Tip + EGFP |
| BBa | Coding | AM.CWP + VHb |
| BBa | Coding | AM.Sed + VHb |
| BBa | Coding | AM.Tip + VHb |
Table 1: New Parts Table