2023.05.01 Establishment of 2024 OUC-Haide Recruitment Group.
2023.05.09 Full-scale launch of 2024 OUC-Haide recruitment work.
2023.06.03 Start of interviews for 2024 OUC-Haide reserve members.
2023.06.17 Confirmation of 2024 OUC-Haide reserve members.
2023.07.05 Start of reserve members meeting.
2023.07.10 Study of protein composition and structure, basic concepts of enzymes.
2023.07.17 Study of nucleic acid structure and DNA replication, reading "A One Pot, One Step, Precision Cloning Method with High Throughput Capability".
2023.07.24 Study of RNA synthesis, processing, and regulation, reading "Capture-SELEX for aptamer selection: A short review".
2023.07.31 Study of protein synthesis and recombinant DNA technology, reading "The Biology of CRISPR-Cas: Backward and Forward".
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Figure 1: Summer School Task List |
2023.08.24 Completion of English and Chinese test papers for the assessment of 2024 OUC-Haide official members.
2023.08.30 Completion of laboratory equipment safety training and passing the laboratory access exam.
2023.09.02 Printing of English and Chinese test papers for the assessment of 2024 OUC-Haide official members.
2023.09.02 Conducting the assessment of 2024 OUC-Haide official members.
2023.09.13 Completion of grading the test papers for the assessment of 2024 OUC-Haide official members.
2023.09.13 Confirmation of 2024 OUC-Haide official core members, 2024 OUC-Haide was officially established today!
2023.09.18 Meeting between 2023 & 2024 OUC-Haide members.
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Figure 2: OUC-Haide 2023 & 2024 OUC-Haide Meeting |
2023.11.05 2023 OUC-Haide won the Gold Medal.
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Figure 3: 2023 OUC-Haide Award Ceremony |
2023.12.07 Group meeting, learning about the specific tasks required for iGEM, determining the first round of excellent Wiki shares for 2024 OUC-Haide core members.
2023.12.14 Group meeting, sharing the first round of Wiki readings, determining the second round of Wiki readings.
2023.12.21 Group meeting, sharing the second round of Wiki readings, determining the third round of Wiki readings.
2024.02.02 Each member registers an iGEM account.
2024.02.04 Group meeting, sharing the third round of Wiki readings, determining the fourth round of Wiki readings.
2024.02.06 Group meeting, sharing readings on Golden and Gibson technologies.
2024.02.08 Group meeting, sharing the fourth round of Wiki readings.
2024.02.21 Joining the 2024 China iGEM group chat.
2024.02.22 2024 OUC-Haide officially starts the project.
2024.03.08 Start recruiting graphic designers.
2024.03.10 First round of project initiation for 2024 OUC-Haide.
2024.03.14 Officially forming 2024 OUC-Haide on the iGEM website.
2024.03.17 Second round of project initiation for 2024 OUC-Haide.
2024.03.20 Confirming two graphic designers.
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Figure 4: 2023 OUC-Haide Award Ceremony at Ocean University of China |
2024.03.21 2024 OUC-Haide volunteers at the "Children of the Sea" exemplary student award ceremony, presenting flowers to 2023 OUC-Haide
2024.03.24 Third round of project initiation for 2024 OUC-Haide.
2024.03.24 Core members share the registration fee.
2024.03.25 Planning to host the 11th CCiC.
2024.03.26 Writing application materials for hosting the 11th CCiC.
2024.03.27 Determining the project theme of β-glucan, starting to organize materials.
2024.03.28 Studying the feasibility of improving Saccharomyces cerevisiae for efficient production of β-glucan.
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Figure 5: β-glucan |
2024.04.01 Core members attempt to pay the registration fee.
2024.04.02 The team communicates with the advisor and summarizes multiple project initiation experiences, confirming project objectives and indicators.
2024.04.02 Confirming the advisor and having in-depth discussions to determine the next steps.
2024.04.05 Changing the chassis organism to marine yeast.
2024.04.05 Preliminary design of a toxin gene suicide switch.
2024.04.05 Core members successfully pay the registration fee.
2024.04.05 Filling out the funding application form.
2024.04.06 Completing the materials for hosting CCiC and submitting them to the college for approval.
2024.04.07 Borrowing GEMs from the related species Aspergillus niger.
2024.04.08 Establishing the basic TPNR framework for HP.
2024.04.09 Confirming communication with SDU-China.
2024.04.09 Using CarveMe and Cobrapy for analysis.
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Figure 6: Metabolic Network |
2024.04.12 Completing the funding application and submitting it.
2024.04.13 Starting to culture marine yeast.
2024.04.14 Several core members go to apply for passports together.
2024.04.15 Submitting CCiC materials for school approval, delayed by the sports meeting.
2024.04.15 Lysing and extracting total DNA from marine yeast.
2024.04.16 Using the OptForce algorithm to identify key reactions and determine secondary metabolites affecting β-glucan synthesis.
2024.04.18 Using Simbiology to confirm gene knockout targets.
2024.04.19 Starting to amplify the melanin gene using PCR.
2024.04.21 Learning that CCiC will be held in Suzhou, abandoning the application process.
2024.04.24 Discussing with the Youth League teacher whether to rely on the college's official account or create our own team account for publicity.
2024.04.25 Finding the melanin gene to be long, designing three pairs of primers for segmented amplification.
2024.04.29 Completing the PCR of the melanin gene.
2024.04.30 Confirming the initial EDU concept.
2024.04.30 Agarose gel electrophoresis identification of PCR products, with many nonspecific bands.
2024.05.02 Re-amplifying the melanin gene in segments using PCR.
2024.05.03 Agarose gel electrophoresis identification of PCR products and recovery.
2024.05.03 Proposing standardized recruitment.
2024.05.04 Starting to establish the sugar metabolism pathway.
2024.05.06 Non-laboratory work officially begins in full swing, with tasks assigned to individuals.
2024.05.08 Connecting the PCR product to the T vector.
2024.05.10 Proposing to conduct HP in conjunction with mangroves.
2024.05.11 Preparing materials for communication with SDU-China.
2024.05.11 Transforming E. coli.
2024.05.12 Screening positive clones and verifying with PCR.
2024.05.12 Communicating with SDU-China.
2024.05.14 Extracting, sequencing, and comparing plasmids.
2024.05.14 Drawing the initial version of the team logo.
2024.05.14 Proposing a team logo based on the Ascension of Christ.
2024.05.16 Confirming participation in CCiC, starting to make team uniforms.
2024.05.19 Verifying the upstream sequence of the melanin gene.
2024.05.20 Starting modeling exploration and optimizing culture conditions.
2024.05.21 Starting to make the CCiC poster.
2024.05.21 Writing the push for communication with SDU-China.
2024.05.21 Filling out the Safety Form and Check-in Form.
2024.05.23 Reflecting on the long road ahead.
2024.05.24 Collecting the list of high schools and universities that have participated in iGEM in the past for EDU preparation.
2024.05.26 Taking group photos for the CCiC brochure.
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Figure 8: Group photo for the CCiC brochure |
2024.05.26 Completing the push for communication with SDU-China.
2024.05.27 Deciding to hold a conference in Wuhan with Wuhan University, Huazhong University of Science and Technology, and HZAU-China.
2024.05.27 The Ascension of Christ logo element is incorporated into the new team logo.
2024.05.27 Starting to construct the melanin gene knockout vector.
2024.05.29 Modifying the Safety Form based on feedback.
2024.05.30 Attending the Math conference.
2024.05.31 Confirming the track is agriculture.
2024.05.31 PCR amplification of the 5’ and 3’ homologous regions of the melanin gene.
2024.02.02 Each member registers an iGEM account.
20242024.06.01 Agarose gel electrophoresis identification of PCR products and recovery.
2024.06.02 Connecting the PCR product to the vector.
2024.06.03 Proposing practical agricultural HP directions for β-glucan.
2024.06.04 Submitting the CCiC reimbursement application.
2024.06.04 Transforming E. coli with the vector.
2024.06.06 Verifying positive transformants.
2024.06.06 Proposing the initial version of the team uniform.
2024.06.09 Lysing E. coli and extracting plasmids.
2024.06.09 Conducting HP at Beihang University.
2024.06.10 Conducting HP at Peking University Cardiovascular Research Institute.
2024.06.10 Conducting HP at HZAU-China.
2024.06.13 Starting to make the CCiC poster.
2024.06.14 Proposing improvements to the team uniform, which eventually becomes the official team uniform.
2024.06.14 Starting to design Hardware.
2024.06.14 Performing double digestion and using DNA ligase to connect the 5’ and 3’ homologous regions, completing the construction of the knockout vector.
2024.06.17 PCR amplification of the knockout vector to obtain linear knockout fragments.
2024.06.18 Agarose gel electrophoresis identification of PCR products.
2024.06.22 Picking single colonies and inoculating into HC medium.
2024.06.22 Filling out various CCiC materials.
2024.06.23 Completing the CCiC poster.
2024.06.23 Reaching an OD600nm of 1.1.
2024.06.23 Performing PEG transformation and culturing on antibiotic medium.
2024.06.24 Obtaining transformants.
2024.06.24 Completing the final team logo and team uniform.
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Figure 8: Team logo |
2024.06.25 Re-screening the transformants.
2024.06.28 Visible transformant colonies are significantly whiter than wild-type colonies.
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Figure 10: Melanin knockout experiment results |
2024.06.28 Wuhan University comes to the school for HP.
2024.06.30 Extracting DNA from marine yeast for genotyping.
2024.07.01 Comparing the relative expression levels of the melanin gene in knockout and wild-type strains.
2024.07.04 Performing RT-qPCR to confirm the decreased relative expression of the melanin gene in the knockout strain.
2024.07.05 Completing the CCiC presentation PPT.
2024.07.06 Discussing culture medium optimization plans.
2024.07.11 Performing infrared spectroscopy analysis of melanin content changes.
2024.07.11 Arriving in Suzhou to attend CCiC.
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Figure 11: Team in CCiC |
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Figure 12: Team in CCiC |
2024.07.12 Performing NMR analysis of melanin content changes.
2024.07.12 Proposing HP can create antibiotic distribution data analysis.
2024.07.14 Conducting stress resistance experiments on knockout and wild-type strains.
2024.07.14 Reporting at CCiC
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Figure 13: Reporing in CCiC |
2024.07.14 Inspired to pay attention to cultural and conceptual differences at home and abroad.
2024.07.14 Proposing the wiki homepage should reflect livestock and feed.
2024.07.15 Starting to construct the pullulan gene knockout vector.
2024.07.17 PCR amplification of the 5’ and 3’ homologous regions of the pullulan gene.
2024.07.18 Agarose gel electrophoresis identification of PCR products and recovery.
2024.07.18 Proposing to participate in the CJUH-JLU-China ethics white paper compilation independently.
2024.07.18 Completing the initial model of the oxygen supply protein.
2024.07.19 Establishing the summer work schedule.
2024.07.19 Starting to summarize and write experimental results.
2024.07.19 Connecting the PCR product to the vector.
2024.07.20 Starting to construct 3D printing models and display diagrams.
2024.07.21 Participating in the JLU-NBBMS ethics white paper compilation.
2024.07.22 Establishing the Wiki production plan.
2024.07.23 Transforming E. coli with the vector.
2024.07.23 Verifying positive transformants.
2024.07.24 Confirming participation in the NNU-CHINA The Light of Biology, Building Future Together podcast.
2024.07.24 Discovering the push for SDU-China was not sent out, finally realizing it was not reported to the college, deciding to send it together with the CCiC push.
2024.07.25 Improving the oxygen supply protein model.
2024.07.25 Going to the Tengzhou Animal Quarantine Declaration Point for HP.
2024.07.25 Completing the antibiotic distribution map
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Figure 14: Antibiotic distribution map |
2024.07.26 Completing the CCiC push draft.
2024.07.26 Lysing E. coli and extracting plasmids.
2024.07.27 Going to the farm for HP.
2024.07.30 Publishing the SDU-China push draft and the CCiC push draft.
2024.07.30 Going to the farm for HP.
2024.08.01 Performing double digestion and using DNA ligase to connect the 5’ and 3’ homologous regions, completing the construction of the knockout vector.
2024.08.02 Completing the initial model of the anchor protein.
2024.08.03 Modifying the Safety form based on feedback.
2024.08.03 Accepting SDU-China's request to come to the school for HP.
2024.08.05 PCR amplification of the knockout vector to obtain linear knockout fragments.
2024.08.06 Agarose gel electrophoresis identification of PCR products.
2024.08.06 Publishing the first mangrove podcast.
2024.08.07 Publishing the second mangrove podcast.
2024.08.08 Optimizing the carbon and nitrogen sources in the culture medium.
2024.08.09 Starting to make the Project Promotion Video.
2024.08.09 Project name confirmed as BEST-ABfree (β-glucan efficient synthesis technology to reduce antibiotic utilization).
2024.08.10 Improving the anchor protein model.
2024.08.10 Picking single colonies and inoculating into HC medium.
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Figure 15: Anchor Protein |
2024.08.11 Wiki production with clickable bubble effect.
2024.08.12 Publishing the antibiotic history podcast.
2024.08.12 Confirming iGEM's participation in the Central China Regional Exchange Conference.
2024.08.14 Discussing modeling with CAU-China, NJTECH-China, and SDU-China.
2024.08.15 Completing the compilation of The Biosecurity and Bioethics Whitepaper.
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Figure 16: Biosecurity and Bioethics Whitepaper |
2024.08.16 Reaching an OD600nm of 1.1.
2024.08.16 Performing PEG transformation and culturing on antibiotic medium.
2024.08.17 Obtaining transformants.
2024.08.18 Communicating with the advisor, deciding to further develop the Art based on Nezha.
2024.08.18 Re-screening the transformants.
2024.08.20 Receiving a named invitation letter.
2024.08.20 Extracting DNA from marine yeast for genotyping.
2024.08.21 Comparing the relative expression levels of the pullulan gene in knockout and wild-type strains.
2024.08.21 Establishing the 2025 OUC-Haide Recruitment Group.
2024.08.22 Performing RT-qPCR to confirm the decreased relative expression of the pullulan gene in the knockout strain.
2024.08.23 Performing HPLC analysis of pullulan content changes.
2024.08.24 Using cre-loxp technology for antibiotic resistance removal to ensure safety.
2024.08.26 PCR identification of successful antibiotic resistance removal.
2024.08.26 Establishing a fusion model of fluorescent protein and anchor protein.
2024.08.27 Starting to write various parts of the text.
2024.09.03 Establishing a model of the interaction between fusion protein and cell membrane.
2024.09.05 Sending the recruitment push to the freshmen group.
2024.09.05 Sending the recruitment push to the school's official account.
2024.09.08 Designing the Home Page.
2024.09.11 Completing the optimization of the carbon and nitrogen sources in the culture medium.
2024.09.13 Testing and improving the Hardware.
2024.09.17 Starting to construct the wiki.
2024.09.20 Completing the Hardware.
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Figure 17: Hardware Diagram |
2024.09.22 Completing the writing of various texts.
2024.09.27 Applying for a French visa.
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Figure 18: Group photo of some members |
2024.09.27 Completing the NoteBook.