NTU iGEM 2024

The iGEM NTU team is dedicated to exploring the evolution of T7 RNA polymerase and its applications in synthetic biology. Our project aims to enhance the efficiency and specificity of RNA transcription processes using engineered variants of T7 RNA polymerase.

T7 RNA polymerase is derived from the T7 bacteriophage and is known for its high specificity towards T7 promoters, making it a vital tool in molecular biology for synthesizing RNA. Its low error rate and rapid transcription capabilities have made it a cornerstone in various biotechnological applications.

Objectives (click here to find out more)

• Develop a Diverse Library: Generate a diverse library of T7 RNA polymerase variants through random mutagenesis.
• Enhance Enzyme Efficiency: Test and characterize these variants to identify those with improved transcription rates and fidelity.
• Applications in Synthetic Biology: Explore potential applications of these engineered enzymes in various synthetic biology projects.

Methodology (click here to find out more)

• Random Mutagenesis: Implement techniques to introduce mutations into the T7 RNA polymerase gene.
• Screening for Variants: Utilize high-throughput screening methods to identify variants with desirable traits.
• Characterization: Analyze the transcription efficiency and error rates of selected variants.

The evolution of T7 RNA polymerase not only contributes to our understanding of enzyme functionality but also paves the way for advancements in synthetic biology applications, including vaccine development.

Stay updated with our progress by following us on our social media platform:
Instagram: @ntuigem

We would like to thank our mentors, sponsors, and collaborators who have supported us throughout this project.