We used two different types of genetic parts, a single basic part and a composite part.
Basic parts are individual pieces of genetic code including, protein sequences, regulatory sequences and promoters. We used a total of 10 different elements
Part Code | Part Name | Parts Registry |
---|---|---|
BB_K5426001 | Linalool Synthase | https://parts.igem.org/Part:BBa_K5426001 |
Bba_K731721 | Bba_K731721 | https://parts.igem.org/Part:BBa_K731721 |
Bba_K3830009 | F1 Ori of Replication | https://parts.igem.org/Part:BBa_K3830009 |
BBa_K5426003 | KanR | https://parts.igem.org/Part:BBa_K5426003 |
BBa_K5426004 | PMBI Ori | https://parts.igem.org/Part:BBa_K5426004 |
BB_K5426005 | Rop | https://parts.igem.org/Part:BBa_K5426005 |
BB_K5426006 | Lac1 | https://parts.igem.org/Part:BBa_K5426006 |
BBA_K4790078 | Lac Operator | https://parts.igem.org/Part:BBa_K4790078 |
BBA_K3988001 | Lac1 Promoter | https://parts.igem.org/Part:BBa_K3988001 |
Note: Parts BBa_K731721, BBa_K3830009, BBa_K4790078 and BBa_3988001 were sourced from iGEM12_UNITN-Trento, iGEM21_NCHU_Taichung, iGEM23_NNU_CHINA and iGEM21_Hong_Kong_HKU respectively
A composite part is a part made from assembling a series of basic parts. We created two total composite parts, designed for expression in E.coli.
Part Code | Part Name | Parts Registry |
---|---|---|
BB_K5426009 | pET28a+ for E.Coli Protein Expression | https://parts.igem.org/Part:BBa_K5426009 |
BB_K5426010 | pET28a+ for Linaool Production | https://parts.igem.org/Part:BBa_K5426010 |