We designed 65 parts for the project, including 39 basic parts and 26 composite parts, covering ALAS genes derived from different species, regulatory elements used in experiments, and RChemA sequences mutated through prediction. Our favorite parts are BBa_K5303001 and BBa_K5303014. The former initially provided us with higher-activity of the ALAS, the latter was built on the former to encode the highest-activity ALAS currently available. Additionally, CASTs-associated pQcasTns(Ptr)-array8 and ptDonor plasmids (BBa_K5303113 and BBa_K5303114) were constructed to address the problem of unstable ALAS free expression during the experiment. Furthermore, enzyme constrained model was employed to predict a series of potential targets and plasmids, such as BBa_K5303103, were constructed for their regulation. All of the parts we use have been submitted to the iGEM Registry following the BioBricks assembly standards. Please find below a brief form for registering our parts. For more information on our parts, please visit our Parts page.