March-April
Project proposal: Brainstorming, project background research, consultation with relevant experts, proposal report.
March-August
Building Controllable Semi-Random Mutagenesis System
High Screening Selection over Human Selection
Development of a monitor to quantify the process mutagenesis
Improvement from GFP to metabolic system evolution using CBE-Mag-RNAP
Safety Consideration before Application on Advanced Life
Implementation of C. elegans
Construction of a Mutation Library Database Design
Potential Future Application in the medical field
May-August
Dry lab design: Plasmid Construction,Sequencing Analysis,Structural Prediction,Metabolic Pathways,Model
June-August Wet lab
-June
Laboratory safety training
experiment design
material preparation
hands-on training of nematodes and Escherichia coli
-July
Plasmid extraction, transformation, and amplification.
Nematode life cycle experiment.
Fluorescence intensity test of CEB-Mag-RNAP colonies.
Gel electrophoresis experiment and quality control of DNA.
Sequencing and sending samples of bacterial liquid.
-August
Flow cytometry sorting based on CEB-Mag-RNAP mutagenesis.
Large-scale extraction of plasmids.
PCR and DNA sequencing library construction.
High-throughput sequencing and analysis.
Modeling
September
Data analysis of experimental results.
Construction of mutation and function information database.
Protein structure prediction.
WIKI construction.
October
Prepare for Presentation
Prepare for Giant Jamboree