Parts

Biobricks	Short Description	Contributions from our team
BBa_K3771048	SoxS Promoter	We investigated promoter performance at different induction temperatures using sodium nitroprusside (SNP) induction.
BBa_K2215003	CuZn SOD	Directed evolution of the original SOD-1 to obtain SOD with higher enzyme activity.
BBa_K5322000	Constitutive Mfp3 Expression System	The plasmid for constitutive expression of Mfp3 was constructed, using BL21(DE3) as the chassis cell for protein expression. Protein characterization was subsequently performed.
BBa_K5322001	Protein Linker (GGGGS)	It is part of a flexible ligand group composed of small non-polar amino acids, such as glycine (Gly), and polar amino acids, such as serine (Ser).
BBa_K5322002	Constitutive Mfp5 Expression System	The plasmid for constitutive expression of Mfp5 was constructed, using BL21(DE3) as the chassis cell for protein expression. Protein characterization was subsequently performed.
BBa_K5322003	Constitutive Mfp53 Expression System	The plasmid for constitutive expression of Mfp53 was constructed, using BL21(DE3) as the chassis cell for protein expression.
BBa_K5322004	SoxS Promoter	A promoter regulated by the SoxR transcription factor and activated by nitric oxide (NO).
BBa_K5322005	NO-inducible Mfp3 Expression System	The construction of a nitric oxide-inducible Mfp3 plasmid, with Escherichia coli Nissle 1917 (EcN) as the chassis cell, involves the SoxS promoter-regulated expression of the protein under high nitric oxide (NO) conditions. Protein characterization was also performed.
BBa_K5322006	NO-inducible Mfp5 Expression System	The construction of a nitric oxide-inducible Mfp5 plasmid, with Escherichia coli Nissle 1917 (EcN) as the chassis cell, involves the SoxS promoter-regulated expression of the protein under high nitric oxide (NO) conditions. Protein characterization was also performed.
BBa_K5322007	NO-inducible Mfp53 Expression System	The construction of a nitric oxide-inducible Mfp53 plasmid, with Escherichia coli Nissle 1917 (EcN) as the chassis cell, involves the SoxS promoter-regulated expression of the protein under high nitric oxide (NO) conditions.
BBa_K5322011	lac-inducible SOD-1 expression system	An lac-inducible SOD-1 expression plasmid was constructed, using DH5α as the chassis cell for protein expression, and enzyme activity was determined.
BBa_K5322013	NO-inducible SOD-1 expression system	An NO-inducible SOD-1 expression plasmid was constructed.
BBa_K5322014	SOD Pro1	An inducible SOD-1 expression plasmid was constructed by single-point mutation of SOD-1 (The 10th base is mutated from glycine to alanine), and protein expression was performed using DH5α as the chassis cell, and enzyme activity was determined.
BBa_K5322015	SOD Pro2	An inducible SOD-1 expression plasmid was constructed by single-point mutation of SOD-1 (The 61st base is mutated from glycine to alanine), and protein expression was performed using DH5α as the chassis cell, and enzyme activity was determined.
BBa_K5322016	SOD Pro3	An inducible SOD-1 expression plasmid was constructed by single-point mutation of SOD-1 (The 64th base is mutated from phenylalanine to alanine), and protein expression was performed using DH5α as the chassis cell, and enzyme activity was determined.
BBa_K5322017	SOD Pro4	An inducible SOD-1 expression plasmid was constructed by single-point mutation of SOD-1 (The 118th base is mutated from valine to alanine), and protein expression was performed using DH5α as the chassis cell, and enzyme activity was determined.
BBa_K5322018	SOD Pro5	An inducible SOD-1 expression plasmid was constructed by single-point mutation of SOD-1 (The 129th base is mutated from glycine to alanine), and protein expression was performed using DH5α as the chassis cell, and enzyme activity was determined.
BBa_K5322019	SOD Pro6	An inducible SOD-1 expression plasmid was constructed by single-point mutation of SOD-1 (The 56th base is mutated from glutamine to asparagine), and protein expression was performed using DH5α as the chassis cell, and enzyme activity was determined.
BBa_K5322020	SOD Pro7	An inducible SOD-1 expression plasmid was constructed by single-point mutation of SOD-1 (The 97th base is mutated from aspartic acid to lysine), and protein expression was performed using DH5α as the chassis cell, and enzyme activity was determined.
BBa_K5322021	SOD Ultra1	An inducible SOD-1 expression plasmid was constructed by single-point mutation of SOD-1 (The 97th base is mutated from aspartic acid to lysine; The 10th base is mutated from glycine to alanine), and protein expression was performed using DH5α as the chassis cell, and enzyme activity was determined.
BBa_K5322022	SOD Ultra2	An inducible SOD-1 expression plasmid was constructed by double-point mutations of SOD-1 (The 64th base is mutated from phenylalanine to alanine, The 118th base is mutated from valine to lysine), and protein expression was performed using DH5α as the chassis cell, and enzyme activity was determined.
BBa_K5322023	SOD Ultra3	An inducible SOD-1 expression plasmid was constructed by double-point mutations of SOD-1 (The 61st base is mutated from glycine to alanine, The 56th base is mutated from glutamine to asparagine), and protein expression was performed using DH5α as the chassis cell, and enzyme activity was determined.
BBa_K5322024	SOD Ultra4	An inducible SOD-1 expression plasmid was constructed by double-point mutations of SOD-1 (The 129th base is mutated from glycine to alanine, The 97th base is mutated from aspartic acid to lysine), and protein expression was performed using DH5α as the chassis cell, and enzyme activity was determined.
BBa_K5322025	SOD Plus	An inducible SOD-1 expression plasmid was constructed by three-point mutations of SOD-1 (The 64th base is mutated from phenylalanine to alanine, The 118th base is mutated from valine to alanine, The 61st base is mutated from glycine to alanine), and protein expression was performed using DH5α as the chassis cell, and enzyme activity was determined.
BBa_K5322030	Programmed Cell Death 1 Ligand 1 [Mus musculus] Expression System	This plasmid is used to express the full-length mouse PD-L1 protein.
BBa_K5322031	Programmed Cell Death 1 Ligand 1 Functional Domain [Mus musculus] Expression System	This plasmid is used to express the truncated functional domain of mouse PD-L1.
BBa_K5322032	Lpp-OmpA-Programmed Cell Death 1 Ligand 1 Functional Domain [Mus musculus] Expression System	This plasmid is used for the surface display of the truncated functional domain of mouse PD-L1 in Escherichia coli, incorporating a GISS protein linker and a TEV site to demonstrate the success of the surface display.
BBa_K5322033	Programmed Cell Death 1 Ligand 1 [Mus musculus]	The mouse PD-L1 sequence, optimized according to the Escherichia coli codon usage table.
BBa_K5322034	Programmed Cell Death 1 Ligand 1 Functional Domain [Mus musculus]	The sequence of the mouse PD-L1 functional domain, optimized according to the Escherichia coli codon usage table.
BBa_K5322035	Lpp-OmpA Surface Display System	The Escherichia coli surface display system can present passenger proteins on the outer membrane of E. coli.
BBa_K5322036	Cleavable Flexible Protein Linker (GISS + TEV site)	Composed of the flexible protein linker GISS and a TEV site, it is used for validation of surface display.
BBa_K5322037	pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-eGFP-T7	The eGFP expression system regulated by the SoxR/SoxS oxidative stress promoter is used to investigate protein expression under the pSoxS promoter at different induction temperatures.