| Part name | Organism source | Enzyme | Function | Comments | Registry Links | References |
|---|---|---|---|---|---|---|
| Basic parts | ||||||
| TAL-Sam8 | Saccharothrix espanaensis DSM 44229 | Tyrosine ammonia lyase (TAL) | Deamination of L-tyrosine to 4-coumaric acid | Based on: BBa_I742142, BBa_K2906011, BBa_K1197011 | BBa_K5443001 | |
| C3H-Sam5 | Saccharothrix espanaensis DSM 44229 | Coumaric acid 3-hydroxylase (C3H) | Hydroxylates coumaric acid to caffeic acid | Based on: BBa_I742105, BBa_K238007, BBa_K2906013 | BBa_K5443002 | |
| C3H-HpaBC | Escherichia coli MG1655 | Hydroxyphenylacetate 3-hydroxylase | Hydroxylates coumaric acid to caffeic acid | Based on: BBa_K1124011, BBa_K4912001, BBa_K4912008 | BBa_K5443003 | |
| COMT-ara | Arabidopsis thaliana | Caffeic acid o-methyltransferase (COMT) | Methylates caffeic acid to produce ferulic acid | Based on: BBa_K2906015 | BBa_K5443009 | |
| COMT-6714 | Synechocystis PCC6714 | Caffeic acid o-methyltransferase (COMT) | Methylates caffeic acid to produce ferulic acid | New Part | BBa_K5443010 | Kunjapur et al 2019 |
| FCS-Ppu | Pseudomonas putida KT2440 | Feruloyl-CoA synthetase | Adds CoA to ferulic acid | Based on: BBa_K1129001 | BBa_K5443011 | |
| FCS-Str | Streptomyces sp. V-1 | Feruloyl-CoA synthetase | Adds CoA to ferulic acid | Based on: BBa_K4256006 | BBa_K5443012 | |
| ECH-Ppu | Pseudomonas putida KT2440 | Enoyl-CoA hydratase/aldolase (ECH) | Hydrolyses Feruloyl-CoA to vanillin | Based on: BBa_K1129002 | BBa_K5443013 | |
| ECH-Str | Streptomyces sp. V-1 | Enoyl-CoA hydratase/aldolase (ECH) | Hydrolyses Feruloyl-CoA to vanillin | Based on: BBa_K4256007 | BBa_K5443014 | |
| Composite Parts – Single genes with RBS added | Organism | Enzyme | Function | Part Number |
|---|---|---|---|---|
| RBS1-TAL-Sam8 | Saccharothrix espanaensis DSM 44229 | Tyrosine ammonia lyase (TAL) | Deamination of L-tyrosine to 4-coumaric acid | BBa_K5443016 |
| RBS2-C3H-Sam5 | Saccharothrix espanaensis DSM 44229 | Coumaric acid 3-hydroxylase (C3H) | Hydroxylates coumaric acid to caffeic acid | BBa_K5443018 |
| RBS2-C3H-HpaB C | Escherichia coli MG1655 | Hydroxyphenylacetate 3-hydroxylase | Hydroxylates coumaric acid to caffeic acid | BBa_K5443020 |
| RBS3-COMT-ara | Arabidopsis thaliana | Caffeic acid o-methyltransferase (COMT) | Methylates caffeic acid to produce ferulic acid | BBa_K5443022 |
| RBS3-COMT-6714 | Synechocystis sp. PCC6714 | Caffeic acid o-methyltransferase (COMT) | Methylates caffeic acid to produce ferulic acid | BBa_K5443023 |
| RBS4-FCS-Ppu | Pseudomonas putida KT2440 | Feruloyl-CoA synthetase | Adds CoA to ferulic acid | BBa_K5443024 |
| RBS4-FCS-Str | Streptomyces sp. V-1 | Feruloyl-CoA synthetase | Adds CoA to ferulic acid | BBa_K5443025 |
| RBS5-ECH-Ppu | Pseudomonas putida KT2440 | Enoyl-CoA hydratase/aldolase (ECH) | Hydrolyzes Feruloyl-CoA to vanillin | BBa_K5443026 |
| RBS5-ECH-Str | Streptomyces sp. V-1 | Enoyl-CoA hydratase/aldolase (ECH) | Hydrolyzes Feruloyl-CoA to vanillin | BBa_K5443027 |
| Composite Parts – Whole gene clusters | Gene Cluster Composition | Part Number |
|---|---|---|
| pMQ3A | ECH-Ppu-FCS-Ppu-COMT-Ara-C3H-Sam5-TAL-Sam8 | BBa_K5443028 |
| pMQ3B | ECH-Ppu-FCS-Ppu-COMT-6714-C3H-Sam5-TAL-Sam8 | BBa_K5443029 |
| pMQ3C | ECH-Ppu-FCS-Ppu-COMT-Ara-C3H-HpaBC-TAL-Sam8 | BBa_K5443030 |
| pMQ3D | ECH-Str-FCS-Str-COMT-Ara-C3H-Sam5-TAL-Sam8 | BBa_K5443031 |