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Colony PCR of E. Coli Single Colonies

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Figure 1: The growth of αPS, βPS and LIMS colonies on petri dish

Figure 2: αPS Colony PCR Gel Electrophoresis Results

Figure 3: AgBS, βPS, LIMS, PaFS, PtPS, SaSS Colony PCR Gel Electrophoresis Results

Figure 4: LIS Colony PCR Gel Electrophoresis Results

Figure 5: MsLIMS, LIMS Colony PCR Gel Electrophoresis Results

coming soon

Terpene Synthase Gene	Expected Size (bp)	Results
AgBS	2454	Successful ✔
αPS	1884	Successful ✔
βPS	1881	Successful ✔
LIS	2613	Successful ✔
LIMS	1671	Successful ✔
MsLIMS	1800	Successful ✔
PaFS	1743	Successful ✔
PtPS	1845	Successful ✔
SaSS	1710	Successful ✔

coming soon

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Sequencing

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Figure 6: Sequencing result Snapgene diagrams of AgBS, αPS, βPS, LIS, MSLIMS, PaFS, PtPS, SaSS, SaSS-CYP736A167

Terpene Synthase Genes	Results
AgBS	Successful ✔
αPS	Successful ✔
βPS	Successful ✔
LIS	Successful ✔
LIMS	Unsuccessful ×
MsLIMS	Successful ✔
PaFS	Successful ✔
PtPS	Successful ✔
SaSS	Successful ✔
SaSS-CYP736A167	Successful ✔

coming soon

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Figure 7: SDS-PAGE protein check gel electrophoresis result of AgBS, SaSS, PaFS, αPS

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Colony PCR of Syn. 6803 Single Colonies

Figure 8: Streak monocultures of transformed Synechocystis sp. PCC 6803 with terpene synthase genes AgBS, αPS, βPS, LIS, MsLIMS, PaFS, PtPS, SaSS, SaSS-CYP736A167

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Figure 9: Colony PCR of AgBS, βPS, PaFS, PtPS, SaSS in Syn. PCC 6803 monocultures

Figure 10: Colony PCR of αPS, SaS-CYP736A167, MsLIMS, LIS in Syn. PCC 6803 monocultures

coming soon

Terpene Synthase Gene	Expected Size (bp)	Results
AgBS	2454	Successful ✔
αPS	1884	Successful ✔
βPS	1881	Successful ✔
LIS	2613	Unsuccessful ×
MsLIMS	1800	Successful ✔
PaFS	1743	Successful ✔
PtPS	1845	Successful ✔
SaSS	1710	Successful ✔
SaSS-CYP736A167	1503	Successful ✔

coming soon

coming soon

coming soon

Figure 9: The GC result of β-pinene and α-Farnesene