coming soon

coming soon

coming soon

• coming soonextract the plasmid with our wanted segments (terpene synthase genes) from product we bought
• coming soonacquire and amplify the segments that we want from the plasmid
• coming soonget rid of unwanted DNA, like the original plasmid, and other unwanted substances from PCR product
• coming soonconnect the targeted segments to the shuttle vector backbone
• coming soontransform the plasmid constructed into competent DH5α E. coli cells
• coming soonto see whether the plasmid is transformed into the E. coli or not, and get the rough size of the transformed targeted segment
• coming soonextract the plasmids from single colonies with positive colony PCR, can be used either for sequencing or for later transformations
• coming soonsend samples to sequencing company and get the exact sequence of constructed plasmid
• coming soon
  • coming soonacquire PCR product with terpene synthase genes
  • coming soonassemble PCR product with linearized pET28a plasmid
  • coming soontransform the plasmid constructed into competent DH5α E. coli cells
  • coming soonto see whether the plasmid is transformed into the E. coli or not, and get the rough size of the transformed targeted segment
  • coming soon extract the plasmid for further transformation into BL21(DE3)
  • coming soontransform previously constructed plasmid into BL21(DE3)
  • coming soonto see whether the plasmid is transformed into the E. coli or not
  • coming sooncultivate the positive single colonies, add IPTG to induce the start of transcription, and the expression of protein
  • coming sooncheck the size of the proteins that were expressed before and after induction
• coming soonto get a chromatogram (chemical composition) showing the products that the proteins have produced
• coming soontransform the plasmid constructed in DH5α into Syn. PCC 6803
• coming soonculture positive monocultures and induce them so production of terpenes can be initiated
• coming soonto check whether the plasmid has been transformed correctly into Syn. PCC 6803 or not
• coming soonculture monocultures that have been transformed successfully and induce them so production of terpenes can be initiated
• coming soonto see the products that the Syn. PCC 6803 has produced through chromatogram