| BBa_K5371000 |
Yeast CYC1 Promoter |
500 |
Regulate expression of downstream genes by HAP4. |
| BBa_K5371001 |
TSC10 |
960 |
Catalyze the reduction of 3-ketodihydrosphingosine, enhance production of ceramides. |
| BBa_K5371002 |
P2A |
57 |
A self-cleaving peptide sequence derived from the porcine teschovirus-1, allowing the generation of multiple independent proteins from a single transcript through ribosomal skipping. |
| BBa_K5371003 |
pRS42H |
8013 |
A yeast expression vector commonly used in synthetic biology and genetic engineering, which includes a hygromycin resistance marker for selection in yeast, an ampicillin resistance marker for selection in bacteria, and a 2-micron origin of replication for high-copy maintenance in yeast cells. |
| BBa_K5371004 |
mCherry |
708 |
A reporter gene in molecular biology, high photostability. |
| BBa_K5371005 |
SIR2 |
1689 |
An NAD-dependent histone and protein deacetylase, which modifies chromatin structure to regulate gene expression. |
| BBa_K5371006 |
GAL4 |
2646 |
A regulatory component in GAL/UAS system. |
| BBa_K5371007 |
ERG20 |
1059 |
Farnesyl pyrophosphate synthetase (FPPS), responsible for the synthesis of farnesyl pyrophosphate (FPP), a critical intermediate for the production of sterols, dolichols, prenylated proteins, and other important biomolecules. |
| BBa_K5371008 |
GGGS3 |
36 |
A short, flexible peptide sequence composed of glycine (G) and serine (S) residues, providing a flexible and hydrophilic connection between two functional units. |
| BBa_K5371009 |
BFP |
719 |
A reporter protein derived from the green fluorescent protein (GFP) family. |
| BBa_K5371010 |
pHXT1 |
800 |
A regulatory DNA sequence found in the yeast Saccharomyces cerevisiae. |
| BBa_K5371011 |
MrBBS |
1719 |
(–)-α-bisabolol synthase, converting FPP to (–)-α-bisabolol. |
| BBa_K5371201 |
pAbAi-GAL4-NH |
7887 |
Report expression of GAL4, while regulating downstream genes through GAL/UAS system. |
| BBa_K5371202 |
GAL4WTV2 |
8616 |
Report expression of GAL4, while regulating downstream genes through GAL/UAS system. |
| BBa_K5371203 |
pESC-URA-MrBBS-BFP |
10192 |
Enables regulated production of (–)-α-bisabolol in yeast by GAL4, while laying basis for real-time reporting of expression level. |
| BBa_K5371204 |
prs42b-erg9 |
7986 |
Swaps the ERG9 promoter in genome of Saccharomyces cerevisiae with HXT1 promoter through homologous recombination. |
| BBa_K5371205 |
pRS42H-TSC10-EGFP |
8790 |
Enables regulated and enhanced production of ceramides in yeast by HAP4, while laying basis for real-time reporting of expression level. |
