February
• February 29
Agenda
- Conducted a brainstorming session based on the specific track.
Notes
- Wu Di and Kong Kexuan: Focused on biomanufacturing using genetic oscillators to produce ethanol and isobutanol.
- Wang Chen, Cao Xiaoran, He Zihang, Xiao Mingyang, Cao Yue, Lv Siyuan, Zhang Xiaohan, Yu Haoyang, and Chen Jinpeng: Explored new therapies for Alzheimer's disease, pancreatic cancer, etc.
- Prof. Huang’s suggestion: Consulted Prof. Chen Ming and voted for the preferred project.
March
• March 10
Agenda
- Selected the project topic: "Production of ethanol and isobutanol based on gene oscillators."
- Brainstormed project details.
- Divided writing tasks for the iGEM project application.
Notes
- Based on the input from Prof. Chen Ming and team voted, the topic "Production of ethanol and isobutanol based on gene oscillators" was selected.
Writing task allocation:
- Background: HP group
- Project design: Dry lab
- Theoretical basis: Wet lab
• March 27–April 1
Agenda
- Wet lab, dry lab, and HP groups conducted interviews and recruited new team members.
Notes
- Each group prepared questions and conducted interviews.
April
• April 9
Agenda
- Full team meeting.
Notes
- iGEM overview (Wang Chen):
1. Timeline & process for iGEM defense.
2. Medals, Villages, and individual awards.
3. Introduction to the offline Grand Jamboree in Paris.
4. Competition fees.
- Introduction of advisors:
1. Prof. Huang Wenwen, Prof. Jin Mengdi, and advisor Zheng Ximeng share their experiences.
2. Prof. Huang emphasized the importance of planning and adhering to deadlines.
3. Prof. Jin highlighted the availability of lab space.
4. Advisor Zheng Ximeng shared insights into building a complete project narrative and leveraging domestic and international resources.
• April 12
Agenda
- Consulted teacher Lian regarding the experimental scheme for longevity yeast.
Notes
- Explored advanced knowledge of ethanol and isobutanol production.
- Acknowledged the challenges in building the genetic oscillator.
- Considered collaborating with Zhouzou’s lab for yeast strains containing oscillators.
• April 24
Agenda
- Switched to the Fashion & Cosmetics Village.
Notes
- The initial idea of ethanol and isobutanol production was derived from an optogenetics paper. However, combining two papers without significant innovation led us to consider alternatives.
• April 28
Agenda
- Meeting with cosmetic expert Dr. Qiao.
Notes
- Ethanol and isobutanol were unsuitable for cosmetics. Dr. Qiao suggested exploring glycolipids and preservatives as alternatives.
May
• May 10–17
Agenda
- Set up the laboratory and conduct access training.
- Began culturing yeast containing the oscillator.
• May 17–31
- Prepared for exams
• May 31
Agenda
- Student leaders' meeting.
- Planned summer vacation tasks for the wet lab, dry lab, and HP groups.
June
• June 11
Agenda
- Student leaders' meeting.
- Reviewed recent work and discuss project issues.
Notes
- Consulted Prof. Chen for feedback on production choices and project specifics.
- Prof. Chen’s suggestions:
1. If uncertain, write vaguely for now.
2. Use well-researched products as a base.
• June 24
Agenda
- Student leaders' meeting.
- Reviewed recent work and discussed project issues.
Notes
- Defined specific schedules for HP and wet lab groups.
- Discussed the hardware design—microfluidic chip.
July
• July 3
Agenda
- Student leaders' meeting.
Notes
- Discussed issues with ceramide AP (sensitive skin) and NP (exfoliation).
- Planned visits to Evonik and BASF for product exchanges.
- Outlined QC, safety, and toxicology testing for products.
- Planned a visit to Guanchen Biotech in August.
- Finalized the story logic for the project and cosmetic product development.
• July 20
Agenda
- Student leaders' meeting.
- Reviewed recent work and discussed project issues.
Notes
- Finalized details for promotion video shooting and script design.
- Discussed microscope use for microfluidic chip observation.
- Wiki group showcased progress.
- Wet lab updated on MrBBS plasmid construction.
- Shifted modeling focused to longevity effects.
August
• August 5
Agenda
- Student leaders' meeting.
- Reviewed recent work and discussed project issues.
Notes
- Discussed French visa requirements.
- Prepared for visits to Guanchen Biotech (August 7) and Ashland (August 9).
- Prof. Chen Di’s feedback:
1. Emphasized the connection between yeast senescence and production.
2. Clarified the necessity of the microfluidic system.
• August 13
Agenda
- Student leaders' meeting.
- Reviewed suggestions from advisors.
- Discussed medal criteria.
September
• September 13
Agenda
- Student leaders' meeting.
- Reviewed recent work and discussed project issues.
Notes
- Wiki group presented the final wiki design.
- Wet lab encountered difficulties in PCR of the specific region, might require a new oscillator yeast strain.
- Modeling and software updated discussed Fast Fourier Transform and LOWESS fit.
October
- Wiki was frozen.