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Notebook

The calendar squeezing all our key dates into one.

February

  • Team recruitment

  • Teambuilding to get to know each other

  • Brainstorming to find a topic

  • Open discussion with our PI about ideas feasibility

March

  • Brainstorming to find a topic

  • Bibliographic research on ideas

  • Discussion of our ideas with SupBiotech professors (Ms Helbecque, Mr Yates)

  • Selection of team leaders and area leaders (Human Practices, Communication, Wet lab, Dry Lab, Education, Entrepreneurship)

  • Visit of the “Salon de l’Agriculture” to learn more about the agricultural industry

  • Meeting with Martine Boccara, viruses specialist at the Museum National d’histoire naturelle (National Natural History Museum)

  • Start of search for partners and investors

  • Visit of the “Forum des Laboratoires” in Lyon to find potential partners

April

  • Bibliographic research

  • Topic validation by our PI

  • Team name and logo brainstorming

  • Beginning of the protocol writing

  • Communication plan writing

  • Communication launch on social media

  • Meeting with DIM BioconvS representative to discuss possible financial collaboration

  • Meeting with the startup RNAway to discuss about RNAi in agriculture (Aviram Avital)

  • Working with primary school pupils to teach them about biology

  • Discovery of our laboratory

May

  • In silico plasmids design

  • Protocol writing

  • Protocol validation by our PI

  • Laboratory inventory

  • Meeting with a sugar beets farmer - Bruno Cardot - to visit his farm and learn more about sugar beets

  • Meeting with a scientific director - Mr Escriou - who worked with sugar beets

  • Visit of the Institut Technique de la Betterave (Sugar Beet Technical Institute) (ITB)

  • Zoom meeting with scientists from l’INRAE: Institut National de la Recherche Agronomique (French National Institute for Agronomic Research) (Veronique Brault, Quentin Chesnais, Martin Drucker)

  • Sugar beets seeds planting

  • Regulatory study

June

  • In silico plasmids designs validation by our PI

  • Participation in a pitch competition at the “Maison des entrepreneurs”

  • Participation in a pitch competition at the Biomim’Challenge during the Biomim’Expo in Paris: “Coup de coeur” prize won

  • Phone meeting with workers from Cristal Union, a sugar cooperative

  • Laboratory preparation for the first experiments, complete the inventory

  • Training for laboratory equipment in the lab

  • Ordering laboratory reagents

  • Preparation of buffer, LB solution

  • Seminar with high school students

  • Algorithm development to verify the safety of our project : SafeRNA

  • Market study and business plan writing

  • Maintenance of sugar beets

July

  • Participation in the Mini Jamboree in Toulouse

  • Participation in the Mini Jamboree in Madrid

  • Participation in the iGEM Startup Summer school

  • Participation in the iGEM meeting about plant bioengineering

  • Participation in the “Innovation Challenge Day” at SupBiotech and won the third price

  • Meeting with Samantha Besse, PhD in bioinformatics

  • Participation at Villejuif Respire

  • Beginning of experiments for the lhRNA (digestion, ligation, transformation) and for the coat protein (virus amplification in plants and protein purification)

  • Algorithm development to predict the arrival of aphids on crops : Aphidisperse

  • Algorithm development to predict the duration of effectiveness of our solution as a function of virus mutations on the targeted sequences: PrediRNA

  • Video game development inspired by our topic

  • Sugar consumers survey

  • Sugar beets industry actors survey

  • Maintenance of sugar beets

  • Meeting with the communication and wiki team to discuss our wiki design

August

  • Experiments for the lhRNA and the coat protein (with amplification of virus in plants and with the golden gate method)

  • Interview with a journalist to write an article on our project

  • Mini jamboree zoom meeting with the Stonybrook iGEM team

  • Maintenance of sugar beets, Nicotiana Benthamiana planting

  • Capsiquizz launching

  • Promotional video production

  • Team member introduction on Instagram

  • Advances in wiki design an page structure

September

  • Yellows virus quantification using qRT-PCR quantification

  • Bacterial transformation

  • Ligation insertion in mCherry bacteria

  • Protein detection assays

  • Wiki layout design

  • Special prizes Selection

  • Interviews with farmers to assess market needs

  • Interviews with several sociology professors on the ethical aspects of our project

  • Maintenance of sugar beets and Nicotiana Benthamiana

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