EXPERIMENT

Year Month Wet-Lab Activity
2022 Jan Determine project
2022 Feb Conducting induced polyploid experiment
2022 Mar Screening and identification of tetraploid seedlings
2022 Apr Planting the first generation of rice WT-4x
2022 May Determine the use of CRISPR/Cas9 gene editing technology to mutate the OsGluB1 gene
2022 Jun Constructing CRISPR/Cas9 gene editing vector targeting OsGluB1
2022 Sept Perform WT-4x fluorescence quantitative analysis
2022 Oct Harvest WT-4x rice and observe the thickness of the endosperm layer and the structures of PSV and PB-II using scanning electron microscopy, Send for testing the total protein, gluten, soluble protein, globulin, and 17 types of amino acids in rice.
2022 Oct Transfer the gene editing vector targeting OsGluB1 into E.coli DH5α for propagation
2022 Nov Extract gene expression vectors and transform them into Agrobacterium. Cultivate WT-4x rice tissue and transform it during the callus stage
2022 Dec Screening and identification of OsGluB1 mutation positive plants
2023 Apr Planting second-generation OsGluB1-4x
2023 Sept Perform OsGluB1-4x fluorescence quantitative analysis
2023 Nov Harvest OsGluB1-4x rice and send it for testing to determine the total protein, gluten, soluble protein, globulin, and 17 types of amino acids in the rice.
2023 Dec Constructing a dual target CRISPR/Cas9 gene editing vector targeting OsGluB1 and OsGlb
2023 Jan Transfer gene editing vector into E.coli DH5α for propagation
2023 Feb Extract gene expression vectors and transform them into Agrobacterium. Cultivate WT-4x rice tissue and transform it during the callus stage
2023 Mar Screening and identification of OsGluB1 and OsGlb mutation positive plants
2023 Apr Planting third-generation OsGluB1/OsGlb-4x
2023 Sept Perform fluorescence quantitative analysis of OsGluB1/OsGlb-4x and harvest OsGluB1/OsGlb-4x rice, Send for testing the total protein, gluten, soluble protein, globulin, and 17 types of amino acids in rice.
2024 Jan Transfer gene editing vector into E.coli DH5α for propagation
2024 Feb Extract gene expression vectors and transform them into Agrobacterium. Cultivate WT-4x rice tissue and transform it during the callus stage
2024 Mar Screening and identification of OsGluB1 and OsGlb mutation positive plants
2024 Apr Planting third-generation OsGluB1/OsGlb-4x
2024 Sept Perform fluorescence quantitative analysis of OsGluB1/OsGlb-4x and harvest OsGluB1/OsGlb-4x rice, Send for testing the total protein, gluten, soluble protein, globulin, and 17 types of amino acids in rice.
Year Month Dry-Lab Activity
2022 Jan-Mar Using the protein and amino acid content data of diploid and tetraploid rice studied by the research group, linear regression is performed to predict the extent of protein and amino acid content
2022 Apr-May Using the research of other rice varieties mutants by the research group and the data of OsGluB1 mutants in the literature, the original linear regression was used as the [WT-2x, WT-4x] segment, and [WT-4x, OsGluB1-4x] was introduced
2022 Jun-Sept Structural prediction of OsGluB1 mutant glutelin and wild-type gluten using designed target sequences
2022 Oct Feedback protein and amino acid content data into a linear regression function for optimizing the [WT-2x, WT-4x] segment functions
2022 Sept-Nov Comparative analysis of the structural characteristics of glutelin before and after mutation, and molecular docking simulation of the hydrolysis of gluten before and after mutation
2023 Nov Feedback protein and amino acid content data into a linear regression function for optimizing the segment functions of [WT-4x,OsGluB1-4x]
2023 Dec Structural prediction of OsGlb mutant globulin and wild-type globulin using designed target sequences
2024 Jan-Mar Using the research conducted by our research group on mutants of other rice varieties and the data of OsGluB1 mutants in literature, we introduce the [OsGluB1-4x, OsGluB1/OsGlb-4x] segment
2024 Feb-Apr Comparative analysis of the structural characteristics of globulin before and after mutation, and molecular docking simulation of allergenic changes in globulin
2024 Sept Feedback protein and amino acid content data into a linear regression function for optimization of the [OsGluB1-4x,OsGluB1/OsGlb-4x] segment functions

HUMAN PRACTICES

2022

February:
Carrying out transgenic public opinion analysis and abandon the strategy of directly reprogramming free lysine metabolic pathway through metabolic engineering.
May:
Carry out a questionnaire survey on domestic and foreign social platforms on the necessity of increasing the lysine content of rice. Instead, adjust the storage protein components through CRISPR/Cas9 gene editing technology, and redefine the technology module to increase the lysine content in the experiment.

2023

November:
Carry out statistical analysis of nutritional composition data of rice and rice paste products on major domestic e-commerce platforms and Sam's Club online flagship stores.
December:
Student leader Ziying Qiu and PI Professor He Yuchi attended the 2023 23rd Jingchu grain and oil boutique exhibition and trade fair.

2024

February:
The team carried out large scale offline market research and science popularization in Hubei, Hunan, Guangdong, Guizhou and Shandong.

March 30:
Yangyu He, the leader of the Dry-team, participated in the Hubei Rural Planning Research Association on behalf of the team.

April 4:
The team went to Wuhan University to participate in the iGEM exchange conference.
April 27:
Organize and participate in the Hubei Synthetic Biology Open Conference with the other three iGEM teams of Hubei University.

May:
The team participated in two speech contests of "Reading at Lake University, Striving for a New Journey" and Baisheng Forum and won two bronzes.
May 18:
Laboratory Open Day

June 1:
Biological Art Exhibition
June 4:
The team participated in the 2024 Wuhan Seed Industry Expo and held a synthetic biology science popularization month carnival.
July 11-July 14:
The team went to Suzhou to participate in the CCiC exchange conference at the beginning of the month.
July 20-July 30:
The team went to Hubei Wuhu Rice Science and Technology Academy for support education

August 8 - August 10:
The team went to Shenzhen to participate in the final of the 2024 Syn-Bio Challenges 3.0
August 17:
Shenzhen University, Tianjin University, University of Geosciences, Sichuan University online exchange HP and security