Parts



Part Lists



New parts


Part ID Type Part Name Short Description
BBa_K5152000 Reporter Constitutive tsPurple Chromoprotein Expression Strong constitutive expression of tsPurple chromoprotein in E. coli
BBa_K5152001 Reporter Constitutive cjBlue Chromoprotein Expression Strong constitutive expression of cjBlue chromoprotein in E. coli
BBa_K5152002 Reporter Constitutive amilCP Chromoprotein Expression Strong constitutive expression of amilCP chromoprotein in E. coli
BBa_K5152003 Reporter Constitutive eforRed Chromoprotein Expression Strong constitutive expression of eforRed chromoprotein in E. coli
BBa_K5152004 Device PbrR-pPbr lead sensing chromoprotein reporter device This device includes the expression of the PbrR repressor, which regulates the pPbr lead-inducible promoter, both of which are part of this construct. In the presence of lead, the pPbr promoter activates the expression of the downstream chromoprotein dTomato, which serves as the reporter signal. This device is specifically designed to detect the presence of lead in the environment.
BBa_K5152005 Device pCadA cadmium sensing chromoprotein reporter device This device includes the cadmium-inducible pCadA promoter along with upstream binding sites for the regulatory proteins ArsR and CzrA. In the presence of cadmium, the pCadA promoter induces the expression of the downstream chromoprotein amilCP, which serves as the reporter signal. This device is specifically designed to detect cadmium in the environment.
BBa_K5152006 Device MerR-pCadA cadmium sensing chromoprotein reporter device This device includes the cadmium-inducible pCadA promoter and co-expresses its repressor MerR, which has been specifically modified to bind cadmium. In the presence of cadmium, the pCadA promoter activates the expression of the downstream chromoprotein amilCP, serving as the reporter signal. This device is specifically designed to detect the presence of cadmium in the environment.
BBa_K5152007 Device pYodA cadmium sensing chromoprotein reporter This device uses pYodA, a cadmium-inducible promoter natively found in E. coli, to drive the expression of the chromoprotein amilCP as the reporter signal. As a result, we did not co-express any regulatory proteins in our design. However, our results showed significant leaky expression even in the absence of cadmium, suggesting that the promoter's repression needs to be strengthened in future designs to improve specificity and reduce background expression.
BBa_K5152008 Device MerR-pMerT mercury sensing chromoprotein reporter device This device contains the mercury-inducible promoter pMerT and co-expresses its repressor protein MerR, which specifically binds to mercury. When mercury is present, the pMerT promoter drives the expression of the chromoprotein tsPurple, serving as the reporter signal. This device is specifically designed to detect the presence of mercury in the environment.
BBa_K5152009 Device pZnt zinc sensing chromoprotein reporter device This device contains the zinc-inducible promoter pZnt, natively found in E. coli, to drive the expression of the chromoprotein eforRed as the reporter signal. As a result, we did not co-express any regulatory proteins in our design. However, our results showed significant leaky expression even in the absence of zinc, indicating that the promoter's repression needs to be strengthened in future designs to improve specificity and reduce background expression
BBa_K5152010 Others 5' 20 nt overlapping region for pUC19 PstI site This part includes overlapping regions on all our inserts, used for the HiFi assembly process.
BBa_K5152011 Others 3' 20 nt overlapping region for pUC19 EcoRI site This part includes overlapping regions on all our inserts, used for the HiFi assembly process.

Existing parts used in this project


Part ID Type Part Name Short Description
BBa_J23100 Promoter Strong constitutive promoter - Anderson constitutive promoter family member This is the strongest promoter among the Anderson constitutive promoter family. We employed this promoter to create composite parts that drive the expression of chromoproteins to study their expression profile.
BBa_J23111 Promoter Medium strength constitutive promoter - Anderson constitutive promoter family membe This is a relatively weaker promoter from the Anderson constitutive promoter family. We employed it to express regulatory proteins for the metal-inducing promoters in our biosensor constructs. A weaker promoter was chosen to potentially increase the sensitivity of our biosensors, though it may introduce the issue of leaky expression.
BBa_K4813000 Coding sequence dTomato - red fluorescent protein codon optimized for E. coli The coding sequence corresponds to the dTomato protein, which produces red fluorescence. It functions as a dimer with a molecular weight of approximately 27.0 kDa, originating from Discosoma. This part, constructed by a previous team and used in various projects, consistently shows strong expression of its color.
BBa_K1033906 Coding sequence tsPurple chromoprotein This coding sequence encodes a deep purple chromoprotein, which we use in our heavy metal biosensors. The visible color allows us to observe the reporter signal without special equipment or techniques, making our biosensor more user-friendly and cost-effective.
BBa_K592012 Coding sequence eforRed chromoprotein This coding sequence encodes a deep red chromoprotein, which we use in our heavy metal biosensors.
BBa_K592011 Coding sequence cjBlue chromoprotein This coding sequence encodes a greenish chromoprotein, which we use in our heavy metal biosensors.
BBa_K592009 Coding sequence amilCP chromoprotein This coding sequence encodes a dark blue chromoprotein, which we use in our heavy metal biosensors.
BBa_B0034 RBS Strong ribosome binding site We included a strong ribosome binding site in our constructs to enable constitutive production of chromoproteins and metal-induced expression of chromoproteins.
BBa_B0015 Terminator Strong double terminator This is the most commonly used terminator in iGEM. We integrated it into our constructs to enable chromoprotein expression and develop heavy metal biosensors.
BBa_K4813005 Reporter J23100 - dTomato red chromoprotein strong expression construct This part was designed by our previous team and has been shown to strongly express dTomato, a chromoprotein that produces a highly visible red color as a reporter signal.
BBa_I721002 CDS PbrR - Lead binding activator for pPbr operon PbrR is a transcriptional activator from the MerR family that regulates the pPbr operon. It responds to the presence of lead ions, undergoing a conformational change upon binding to lead, which activates transcription from the pPbr promoter. We used this in our lead biosensor design.
BBa_I721001 Promoter pPbr- Lead inducible promoter When lead is present, it binds to PbrR, which activates the expression of the pPbr promoter. We leveraged this operon to create our lead-detecting biosensor by driving the expression of the chromoprotein dTomato in the presence of lead.
BBa_K174015 Promoter pCadA - Cadmium inducible promoter with modified repressor binding sites This promoter was originally constructed by the Newcastle 2009 iGEM team. They enhanced the native pCadA promoter by adding ArsR and CzrA repressor binding sites upstream, aiming to increase its specificity in response to cadmium. We utilized this modified operon in our cadmium biosensor, intending to drive the expression of the chromoprotein amilCP when cadmium is present. However, for reasons still unclear, we were unable to successfully assemble the system before the project deadline.
BBa_K1724000 Promoter pCadA - Cadmium inducible promoter This cadmium-inducible promoter is regulated by the MerR protein, which acts as a repressor. We used this system to design one of our cadmium biosensors, incorporating co-expression of the modified MerR protein. The MerR protein was engineered to bind specifically to cadmium, allowing for precise detection and response to cadmium in the environment.
BBa_K1724002 CDS MerR - Modified cadmium binding repressor protein of pCadA The MerR protein was originally a repressor that responded to mercury. However, the SCUT iGEM 2015 team modified MerR to bind specifically to cadmium. In our project, we utilized this modified MerR in conjunction with the pCadA promoter to construct a cadmium biosensor.
BBa_K896008 Promoter pYodA - Cadmium inducible promoter This cadmium-inducible promoter we used is natively found in E. coli. As a result, we did not co-express any regulatory proteins in our design. However, our results indicated significant leaky expression even in the absence of cadmium, suggesting that the promoter's repression needs to be strengthened in future designs to improve its specificity and reduce background expression.
BBa_K346002 Promoter pMerT - Mercury inducible promoter This is a mercury-inducible promoter that functions with the MerR repressor protein. We utilized this promoter in the design of our mercury biosensor. Unfortunately, due to a mistake in our DNA ordering process, we were unable to perform any tests on this system.
BBa_K190016 Promoter pZnt - Zinc inducible promoter This zinc-inducible promoter is natively found in E. coli, so we did not co-express the regulatory protein (ZntR) in our design. However, this likely contributed to the significant leaky expression we observed in the control setup without zinc. This suggests that the promoter's repression needs to be enhanced in future designs to improve specificity and reduce background expression.
BBa_K346002 CDS MerR - Activator protein of pMerT This part encodes the MerR regulatory protein, which functions as both a repressor and an activator for the pMerT promoter. In the absence of mercury, MerR binds to the promoter and represses transcription by distorting the DNA. When mercury is present, MerR undergoes a conformational change, which activates the pMerT promoter, allowing transcription of downstream genes. We co-expressed this part in our mercury biosensor design in conjunction with pMerT.