Ayush DALMIA

1. Background Research - Researched PEX10 Knockout and Nile Blue/ Red Lipid Staining
2. Protocol Optimisation- Designed and optimised the protocol for Nile Blue/ Red Lipid Staining
3. Writing: Crafted Wiki content outlining my diverse involvements and experiences. I reviewed and enhanced Wiki materials focused on Integrated Human Practices and Education, playing a pivotal role as a primary proofreader in ensuring accuracy and clarity
4. Other- Education & Public Engagement - Organised and held educational events such as the high school workshop series, YSIP, etc. Served as the primary YSIP tutor in both the wet lab and circuit design modules. Collaborated on YouTube videos featuring team members and professors, and authored compelling LinkedIn articles

Constance, Cancan ZHANG

1. Analysis - Analysed the experimental data from DHA standard and lipid staining
2. Investigation - Performed experiments on cloning PPTase, transformation, lipid extraction, protein extraction, lipid staining, and hardware
3. Background research - Researched about lipid extraction, purification, and DHA detection

Henry, Cheuk Hin CHOY

1. Project Administration - Managed and coordinated project activities, including planning and execution
2. Public Engagement - Involved in most of the outreach events, from requesting meetings to actual meetups. Served as the main person in charge of collaboration with CUHK and HKU for the Hong Kong-Macau iGEM Symposium and the Asia-Pacific Regional Mini Jamboree
3. Team Communication - Managed communications for the team, including overseeing the mailbox and social media platforms
4. Public Relations - Established connections with third parties to facilitate collaborative events, including regional iGEM symposiums and career-building sessions for the Youth Scientist Incubation Program

Diana, Daeun SUH

1. Background Research- Researched on YAC transformation using electroporation, and growth medium optimization with phosphate concentration regulation
2. Investigation - Designed and conducted experiments for GC-MS for lipid profiling
3. Visualisation - Designed presentation materials, poster, social media posts and goods for public engagement, animated promotional video, and designed UI of the Wiki

Dayeong YOON

1. Background research - Researched papers about the lipid extraction method and PUFA Synthase
2. Wiki coding - Coded the 2024 Hkust iGEM website by programming language.
3. Investigation - Designed and carried out tests for GC-MS for lipid profiling

Angel, Hei Ching IU

1. Analysis - Analyse experimental data of growth curve and 1L-fermentor experiments
2. Investigation - Designed and conducted experiments for yeast growth curve, helped with cloning experiments
3. Software - Worked on DHA yield prediction using MATLAB and genome scale metabolic models
4. Writing - Wrote part of Dry Lab wiki; notebook, protocols and design for Growth Curve Experiments
5. Background Research - Conducted research on PEX10 and lipid staining
6. Public Engagement - Taught YSIP and managed Youtube as one of the education tools by shooting, editing and uploading videos related to synthetic biology or our project
7. Other - Education - Organized workshop 3 and conducted interview with professors
8. Investigation - Assisted with cloning in lab

Aurora, Jingjun ZHU

1. Analysis - Analysed experimental data of cloning and transformation (E. coli and Y. lipolytica)
2. Background Research - Researched on PEX10 Knockout and Y. lipolytica transformation
3. Investigation - Designed and conducted experiments for cloning and transformation (E. coli and Y. lipolytica)
4. Notebook/record keeping - Maintained the experiment logbook and summarised into a notebook

Amanda, Liu LIANG

1. Education - Held different educational event such as UST booth, primary school workshops and JSSE
2. Entrepreneurship - Conducted market research and developed business model based on business model canvas
3. Investigation - Collected data/evidence during building up business model, specifically cost and revenue estimation and for storyline background support
4. Background Research - Read peer-reviewed scientific research articles, white papers, regulations/guidelines, and other documents

Jenny, Lok Yi CHEUNG

1. Background Research - Helped in researching the storyline and background, including why DHA is beneficial for the brain and the optimum environment for microalgae and yeast. Also researched YAC transformation and assisted in developing the protocol for transformation and selection of PUFA synthase
2. Writing - Wrote wiki content for the activities I have been involved in. Reviewed and edited content for the Wiki related to Human Practices and Education
3. Other - Education - Organized and participated in various educational events, including the Science Museum Biodiversity Workshops and the Joint School Science Exhibition (JSSE) Workshop at Caritas Lok Yi School, among others. I was one of the main individuals in charge of the Youth Scientist Incubation Program (YSIP)
4. Visualisation - Designed promotional materials for most of the educational events, such as an email banner for YSIP and a booklet for the Science Museum Biodiversity Workshop. Created visuals for Instagram and LinkedIn, and designed project posters

Danny, Ming Chun WU

1. Conceptualization - Helped us come up with the initial idea for the project (DHA production by yeast)
2. Wiki coding - Coded the 2024 Hkust iGEM website by Html, CSS and JavaScript
3. Project administration: Managed Deliverables module project activities and work distribution, e.g, wiki coding, design, etc. Also in charge of the wiki content for different modules

Theresa, On Yee LEUNG

1. Background research - Researched about the optimum yeast growth conditions and the reactor design
2. Investigation - Conducted growth experiment in bioreactor
3. Writing - Wrote part of the Dry Lab wiki content
4. Software - Wrote yeast growth model, simbiology model of the reaction pathway

Andrew, Qinghua HUANG

1. Background research - Read paper regarding the project
2. Writing - Wrote notebook for wet lab
3. Investigation - Did experiment including cloning, transformation

Samantha Kaitlyn NG

1. Background Research - Helped in researching the initial NADPH replenishment strategies as well as during troubleshooting for Yeast Transformation
2. Investigation - Initially aided in cloning PPTase genes, Responsible for pinpointing successful yeast transformation protocol
3. Notebook/Record Keeping - Continuously and promptly updated Lab Logbook after each experiment
4. Visualization - Storyboard for promotional video, side page design for wiki (Dry Lab and IHP side page designs), helped create ideas and choices for logo design and web page design

Yanni, Smile Yan Yu YUAN

1. Writing - Helped to write the wiki for outreach sessions, primary school workshop, red and WeChat
2. Other - Education - Hold the event for primary school workshop and inclusivity workshop, preparing the material content for both
3. Public Engagement - Make post for Wechat and RED other events, engaged with the public in JSSE, engaged with kids as audience for Primary school workshop and Arts meet Science inclusivity workshop
4. Analysis - Analysed survey result for market research

Chloe, Suet Wa CHAN

1. Background Research - Read through research papers understanding the mechanism of the production of DHA during the initial stage of ideation, readed peer-reviewed scientific research articles regarding the NADPH replenishment (gapC gene)
2. Public Engagement - Implemented education tools, such as VR headsets, legos, micropipette, mentimeter, etc. metres to engage the younger generation's awareness on Synbio. Planned and executed activities to engage the broader community, such as CU symposium, highschool workshop series, street surveying, science museum workshop, YSIP, etc.
3. Writing - Wrote, reviewed or edited content for the Wiki regarding the events that I was involved with, like YSIP, mainland university's iGEM team visit, high school workshop series, etc. Also, I helped research on the price of reagents and chemicals needed to be used for several protocols.
4. Other - Education - Held multiple educational events across several months, such as the high school workshop series in April, YSIP during summer, science museum workshop, meme submission on Instagram, reels filming, etc.

David, Tianyue WU

1. Investigation - Designed and performed part of the cloning experiments, collected evidence for justifying the designs and understanding pathway mechanisms
2. Public Engagement - Reached out to professionals and other iGEM teams for their advice and assistance
3. Writing: drafted the project design, engineering and part descriptions on Wiki, helped organise the team presentations

Emily, Wai Ying OU

1. Project Administration - Organised, planned and prepared materials, like posters, PPT for iHP events, including primary school workshops, joining CCiC (11th Conference of China iGEMer Community) and co-host for the 2nd iGBA(iGEM Greater Bay Area Synthetic Biology Industry-Academic-Research Forum)
2. Public Engagement - Mad posts for the Wechat and Red accounts, primary workshops, communicate with other iGEM teams, collaboration with other iGEM teams holding activities, like 2nd iGBA and online education workshop
3. Writing - Wrote for the wiki, the IG post for the iGBA event in official iGBA account
4. Other - Was the secretary of the HKUST iGEM 2024 team

Kathy, Yanlin SUN

1. Background Research - Did research on the PUFA pathway and function of each subunit, the promoters’ yield and inducers, etc.
2. Investigation - Did investigation on cloning, Gibson assembly, competent cell preparation, growth curve, etc.

Selina, Yanyu CHEN

1. Background research - Researched about the mechanism and pathway of PUFA synthase
2. Investigation - Did gBlocks cloning, growth curve, lipid staining and other experiments
3. Software - Modifications to the genome-scale metabolic model for DHA flux prediction
4. Writing - Wrote the DHA production pathway mechanism for Dry Lab

Chloe, Yerim SONG

1. Background research: Researched papers about the lipid extraction method
2. Investigation: Has conducted experiments for obtaining gene fragment(s) and lipid profiling
3. Notebook/record keeping: Kept record of experiment progresses in docs & word document
4. Visualization: Designed a logo, Instagram posts, posters wiki side page titles and icons, jointly created a promotional video

Robert, Zhengyu DING

1. Conceptualization - Helped the team come up with the initial idea for the project during ideation stage
2. Other - Education - Planned and facilitated in various education events such as primary school workshops, science museum workshops, DHA recipe, and online science articles
3. Analysis - Conducted background research to define our problem statement, provide support for the significance of our project, and identify our limitations
4. Entrepreneur - Facilitated with entrepreneur related tasks such as market research, company outreach, financial projections, etc.

Changxin FAN

1. Analysis - Assisted in and provided suggestions for the analysis and interpretation of experimental results
2. Investigation - Provided valuable suggestions and resources for our cloning experiments
3. Writing - Proposed modifications in the wiki contents

Owen, Ching Nam FUNG

1. Investigation - Helped us review experiment plans and provided suggestions
2. Analysis - Assisted in and provided suggestions for the analysis and interpretation of experimental results
3. Background Research - Provided suggestions on construct designs
4. Other - Tutorial: Designed and conducted initial Wet Lab tutorials

Terry, Chun Pan PANG

1. Software - Provided suggestions and reviewed our MATLAB models
2. Investigation - Provided assistance for lipid profiling experiments

Agnes, Hoi Ying LAU

1. Software - Provided suggestions and reviewed our MATLAB models
2. Hardware - Provided suggestions and reviewed our bioreactor
3. Other - Tutorials: Designed and conducted initial Dry Lab tutorials

Sylvia, Hsi Chen CHEN

1. Other - Provided useful tools for wiki coding and provides wiki tutorial

Kei Hong WONG

1. Background Research - Helped us refine the storyline and background
2. Conceptualization - Suggested using cost-effectiveness as one of the selling points of our project
3. Other - Provided comments and feedback on our educational events

Coby, Man Yee NGAI

1. Background Research - Helped us refine the storyline and background
2. Visualisation - Provided suggestions on our visual materials, including posters, wikis pages, and PowerPoint
3. Other - Provided comments and feedback on our educational events

Carmen, Nga Man WONG

1. Software - Provided directions and reviewed our MATLAB models
2. Hardware - Provided reference materials and feedback on the growth curve experiment
3. Other - Tutorial: Designed and conducted initial Circuit Design tutorials

Jason, Sen YEUNG

1. Investigation - Helped us review experiment plans and provided suggestions
2. Background Research - Provided suggestions for our storyline and construct designs
3. Entrepreneurship - Provided feedback and comments regarding our business model guided us to utilise financial tools and find reliable financial statements

Sherman CHAU

1. Analysis - Assisted in and provided suggestions for the analysis and interpretation of experimental results
2. Background Research - Provided suggestions on construct designs
3. Conceptualization - Provided guidance on the project design and development
4. Other - Tutorial: Designed and conducted initial Circuit Design tutorials

Noel, Wing Hei CHOY

1. Writing - Modified, reviewed and edited the wiki content for IHP. Provided constructive feedback on the wiki content
2. Data Curation - Provided tools for us to conduct survey analysis, and offered feedback and suggestions on our survey result

Prof. Jessica TANG

1. Public Engagement - Connected us with relevant stakeholders
2. Conceptualization - Offered suggestions for event planning and experimental design
3. Project Administration
4. Fundraising - Assisted in coordinating project activities, including claiming funds for events and equipment borrowing, ensuring smooth execution of our initiatives

Prof. King CHOW

1. Analysis - Provided feedback regarding our lab results
2. Conceptualization - Provided feedback on project presentation and technical aspects, aiding in the analysis of our project's feasibility and direction
3. Project Administration - Helped manage project funding and provided oversight on financial decisions related to project activities
4. Fundraising - Contributed to fundraising efforts to support the financial aspects of the project

AlGreen

1. Analysis - Shared insights on the limitations of current fermentation and lipid extraction methods for microalgae and discussed how these limitations could inform our project direction
2. Investigation - Provided feedback on lipid extraction processes and gave us tips on refining the protocol to prevent contamination
3. Entrepreneurship - Shared their experience and views towards commercialising a project idea and turning it into a real product

Dr. Nicholson SIU (Neuropsychologist, Lecturer from HKUST)

1. Data Curation - Guided us in creating and refining a survey for market research, ensuring that it collected relevant data effectively for future analysis
2. Public Engagement - Helped refine survey questions to gather feedback from the public, which is crucial for understanding community perceptions of our project
3. Project Administration - Assisted in coordinating the survey process, ensuring that it aligned with our project goals
4. Writing - Reviewed and provided feedback on the survey content to ensure clarity and effectiveness in gathering public opinions

Dr. Aftab AMIN (Lecturer from HKUST)

1. Other - Provided VR headsets as educational tools for the Youth Scientist Incubation Program (YSIP), facilitating hands-on learning experiences related to brain function

Dr. Bruce CHEN (Technical officer from HKUST)

1. Investigation - Provided us 1-L Bioreactor to do the growth curve experiment and offer technical training

Dr. Fei DU (PhD from NNU)

1. Investigation - Kindly provided us with the Y. lipolytica Po1f strain
2. Conceptualization - Provided useful suggestions on the part and experimental designs
3. Background Research - Updated us with the latest progress and challenges met for PUFA production in Y. lipolytica

Dr. Mike CHEN (Manager from HKUST Office of Knowledge Transfer)

1. Conceptualization - Offered insights into entrepreneurship, suggesting avenues for market exploration and the importance of both B2B and B2C strategies
2. Entrepreneurship - Provided insights into local market dynamics and suggested strategies for developing business models relevant to our project’s commercialization potential

European Food Safety Authority

1. Analysis - Provided safety assessment information regarding genetically modified organisms (GMOs) that influenced our project's risk management strategies
2. Background Research - Supplied relevant guidelines and regulations concerning GMOs, which helped inform our project’s compliance and communication strategies

Ms. Queenie LAM (Nutritionist, lecturer at HKBU)

1. Analysis - Provided expertise and insights on the nutrition landscape and DHA related challenges in HK
2. Public Engagement - Shared our project recipe on @negimenforall Instagram page

Ms. Vivain LI (Manager from HKUST Office of Knowledge Transfer)

1. Public Engagement - Advised on patent application considerations, enhancing our understanding of intellectual property in relation to public engagement and commercialization
2. Entrepreneurship - Offered guidance on whether to apply for a patent, contributing to our entrepreneurial strategy by addressing intellectual property concerns

Prof Joseph WONG (Professor from HKUST)

1. Analysis - Helped us understand the evolutionary and regulatory mechanism involved in metabolism
2. Data Curation - Suggested the potential use of resources like the Saccharomyces Genome Database (SGD)

Prof Marshall LIU (Professor from HKUST)

1. Hardware - Provided assistance and suggestions for 1L-fermentor experiments
2. Conceptualization - Shared insights on the limitations of algae growth and discussed our project's competence

Prof Chao Ye

1. Software - Provided advice regarding the use of MATLAB-based COBRA toolbox for genome-scale metabolic models, and suggested the use of AI tools for predicting enzyme-catalysed reaction coefficients involved in our kinetic model.

Prof. Carol PUIG

1. Conceptualization - Assisted in modifying the storyline of our project to enhance its clarity and engagement potential for various audiences

Prof. David BANFIELD (Professor from HKUST)

1. Investigation - Searched for method to ensure the expression of target enzymes e.g. with HA tag and Myc epitope as alternative; and provided suggestion on using auxotrophic marker to address the concern for transformation process, recycling of markers through incorporation of loxP sites
2. Conceptualization: Inspired us to implement the stage control strategy to avoid negative impacts due to constitutive expressions

Prof. Henry LAM (Professor from HKUST)

1. Software - Provided advice regarding making reasonable assumptions and simplifying the DHA yield prediction model
2. Investigation - Discussed on the yeast growth model and the variables necessary

Prof. Karl TSIM (Professor from HKUST)

1. Entrepreneurship - Advice on exploring intellectual property protection + reaching out to potential manufacturers in mainland
2. Investigation - Provided guidance on technical priorities e.g. complexity of enzymatic pathways involved in DHA production

Prof. Lee Joon FOO (Research Assistant Professor from NUS)

1. Conceptualization - Supported the choice of Y. lipolytica as chassis
2. Investigation - Kindly provided us with the pSL16-CRE-HPH plasmids for Cre recombinase expression

Prof. Yong LAI (Professor from HKUST)

1. Analysis - Provided practical suggestions regarding expression of the PUFA synthase cluster and prediction of promoter activities in >Y. lipolytica
2. Investigation - Proposed alternative strategies, such as using yeast artificial chromosomes for the expressions

Prof. Rachubinski (Professor from University of Alberta)

1. Background Research - Provided us with valuable insights into the role of PEX10 and peroxisome in Y. lipolytica
2. Analysis - Supported the idea of optimising DHA production through engineering the beta-oxidation pathway
3. Investigation: Kindly provided us with the anti-SCL antibody for detection of peroxisome biogenesis activity in Y. lipolytica

Researcher WANG Dapeng

1. Public Engagement - Advised us on how to conduct effective science popularisation

Sunny, Seung Yeol YOON

1. Writing - Wrote the wiki for outreach, reviewed and editing the wiki content for IHP and education
2. Public Engagement - Involved in outreach and helped to contact different stakeholders

Caritas Lok Yi School

1. Provided opportunity for us to conduct a workshop focused on using art to teach students with disabilities about synthetic biology and have a collaborative art gallery displaying students' work from the workshop

The Hong Kong Science Museum

1. Provided a venue for workshops and supported the planning and execution of educational activities

Munsang College

1. Helped promote YSIP and facilitated an informative talk on synthetic biology at their school. Discussed on future collaboration opportunities i.e. providing tutorial for students who are interested in iGEM or synthetic biology

SKH Bishop Mok Sau Tseng Secondary School

1. Helped promote YSIP and facilitated an informative talk on synthetic biology at their school. Discussed on future collaboration opportunities i.e. providing tutorial for students who are interested in iGEM or synthetic biology

Dr. Young-Kyoung Park (Researcher from INAS France)

1. Investigation: Offered us useful suggestions for the transformation of Y. lipolytica
2. Background research: Helped us understand the existing technical and regulatory challenges in Y. lipolytica bioproduction

Dr. Zhu Yuanmin (Researcher from Huazhong University of Science and Technology)

1. Entrepreneurship: Shared with us modes of collaborations, regulations, considerations and challenges met in the current microalgae DHA production industry, suggested potential business models and directions of our project

Prof. Chen Zhi (Professor from China Agriculture University)

1. Conceptualization: Confirmed the potential of expressing PUFA synthase system to product DHA in Y. lipolytica
2. Analysis: Shared with us the research experiences and challenges met to improve current DHA production from microalgae

Prof. Rodrigo Ledesma-Amaro (Professor from Imperial College London)

1. Investigation: Provided us useful suggestions for the transformation of Y. lipolytica
2. Background research: Helped us understand the advantages and technology of using Y. lipolytica in the current industry

Prof. David L. Silver (Professor from Duke-NUS medical school)

1. Background research: Provided us useful insights into the DHA membrane transport and its absorption in human body
2. Conceptualization: Provided us valuable suggestions on the possibility of applying DHA membrane transporters in our project, and potential advantages of using Y. lipolytica for DHA production

Prof. Ho Yi Mak (Professor from HKUST)

1. Kindly provided us with fatty acid standards for the GC-MS tests, and offered us suggestions on peroxisomal inhibition

Mr. Mehmet Can Sucu (Founder of ALGDEHA)

1. Analysis: Suggested the potential of our project in the current DHA production industry
2. Entrepreneurship: Provided us with important information including relevant data of the current industry; suggested useful startup and commercialization strategies

Mr. Ziyun Ye (PhD candidate from HKUST)

1. Provided useful suggestions on yeast transformation

Health, Safety, Environment Office

1. Reviewed our project safety and posed insightful questions for us to consider during future applications

Prof. Ren Jian

1. Provided advice for the commercialization of our project

Tsang Mui Millennium School

• Public engagement- Provided a venue for workshops and supported the planning and execution of educational activities

Lee Chi Tat Memorial School

• Public engagement- Provided a venue for workshops and supported the planning and execution of educational activities

University

• Sustech-Med
• HKUST (GZ)
• HongKong-CUHK
• CUHK-HongKong-SBS
• HKU-HongKong
• BNUZH-China
• CityU-HongKong
• HKPOLYU
• UM-Macau
• XJTLU-CHINA
• CAU-China
• Fudan
• Imperial-College
• JLU-NBBMS
• CCU-Taiwan
• SCUT-China-S
• NU-Kazakhstan
• Patras

High School

• HK-United
• HongKong-UCCKE
• HK-Joint-School