General lab safety rules
We participated in safety training sessions provided by Zhejiang University and adhered to the university's Laboratory Safety Management Measures and Laboratory Safety Manual. Only those who have successfully passed the laboratory safety test are allowed access to the lab. All activities and materials within the laboratory are strictly connected to the experiments in progress.
1. Wear disposable gloves and always wear the lab coat properly.
2. Waste liquids should be disposed of in waste tanks, and eating or drinking in the lab is prohibited.
3. Smoking is prohibited.
4. Taking samples out of the lab is forbidden.
5. Get familiarized with emergency escape routes, first aid kit locations, fire extinguishing equipment, eyewash stations, and showers.
6. Wash hands before leaving the lab, and do not wear lab coats or gloves to public places like restaurants or offices.
7. Keep the lab clean and tidy, and consult the lab manager or instructor for any questions rather than operating blindly.
Aeromonas hydrophila safety rules
A. hydrophila is a Risk Group 2 microorganism that can cause septicemia through wounds and gastroenteritis when ingested (Chang et al., 1997; Horneman et al., 2007). Team members must wear lab coats, gloves (changed after handling), and, optionally, masks.
1. The bacteria should be stored in properly labeled, leak-proof containers and are not permitted to leave the laboratory.
2. Containers should only be opened in a Class II biosafety cabinet in a BSL-2 lab and must be sealed before removal.
3. After use, the UV light in the biosafety cabinet should be turned on.
4. Use of needles, syringes, and sharp objects with A. hydrophila is strictly prohibited.
5. If spills occur, cover them with paper towels and disinfectant, allowing adequate contact time before cleaning up.
6. All materials that have been in contact with the bacteria must be autoclaved.
7. Team members working with A. hydrophila should monitor their health and report symptoms such as abdominal pain, diarrhea, or vomiting for medical evaluation (Public Health Agency of Canada, 2004; 2011).
Bacillus subtilis safety rules
Although B. subtilis is generally considered safe for humans, it forms endospores that are hard to kill and can easily spread, so leakage prevention is crucial.
1. All bacterium containers should be sealed with Parafilm and kept in an incubator, and should only be opened in biosafety cabinets.
2. After use, disposables such as gloves, pipette tips, Petri dishes, and microcentrifuge tubes must be autoclaved before disposal.
3. UV lamps should be turned on in biosafety cabinets for at least 40 minutes after use.