Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_010473	4,296,510	671 bp→GAGCGGCCGCCACCGCGGGAAGTTCCTATACTTTCTAGAGAATAGGAACCTTCGCATGCACGCAGCATATGC	coding (2‑672/672 nt)	nfi →	deoxyribonuclease V

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_010473	4296510	4297180	671	38 [0]	[0] 38	nfi	deoxyribonuclease V

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_010473	= 4296509	0 (0.000)	27 (1.100) +GAGCGGCCGCCACCGCGGGAAGTTCCTATACTTTCTAGAGAATAGGAACCTTCGCATGCACGCAGCATATGC	26/254	0.1	100%	coding (1/672 nt)	nfi	deoxyribonuclease V
	?	NC_010473	4297181 =	0 (0.000)					intergenic (+1/‑42)	nfi/yjaG	deoxyribonuclease V/YjaG family protein

CTGGCCTGGGTCTGGCGCAGCAAAGCGCGCTGTAACCCGTTGTTTATCGCTACCGGCCATCGGGTCAGCGTGGACAGCGCGCTGGCGTGGGTACAACGCTGCATGAAAGGCTATCGTCTGCCGGAGCCAACGCGCTGGGCGGACGCGGTGGCCTCGGAACGTCCGGCGTTCGTGCGCTATACAGCAAATCAGCCCTAATTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACAAAACCCAATTCATCTGCGTCTGGAGCGCCTGGAAAGCTGGCAGCATGTCACTTTCATGGCTTGCTTATG‑CGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCA  >  NC_010473/4296983‑4297373                                                                                                                                                                                                       |                                                                                                                                                                                                         acggcgaaggtcatgtctttaaccttggtcacggcattcatcaggatgtgccgccagaacatgctggcgtgttcgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatactttctagagaataggaaccttcgcatgcacgcagcatatgctt                                                                                                                                                                                                         <  1:14614S4‑M2/2‑1 (MQ=255)   ggcgaaggtcatgtctttaaccttggtcacggcattcatcaggatgtgccgccagaacatgctggcgtgttcgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatactttctagagaataggaaccttcgcatgcacgcagcatatgcTTCa                                                                                                                                                                                                       >  1:2590S55‑M2/197‑200 (MQ=255)    ggcgaaggtcatgtctttaaccttggtcacggcattcatcaggatgtgccgccagaacatgctggcgttcgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatactttctagagaatagggaaccttcgcatgcacgcagcatatgcTTCA‑g                                                                                                                                                                                                     <  1:18753S1‑M2/5‑1 (MQ=255)        aggtcatgtctttaaccttggtcacggcattcatcaggatgtgccgccagaacatgctggcgtgttcgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatactttctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTaa                                                                                                                                                                                                 <  1:15710S7‑M2/9‑1 (MQ=255)                gtctttaaccttggtcacggcattcatcaggatgtgccgccagaacatgctggcgtgttcgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatactttctagaaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGa                                                                                                                                                                                     <  1:2793S16‑M2/17‑1 (MQ=255)                ctttaaccttggtcacggcattcatcaggatgtgccgccagaacatgctggcgtgttcgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaattttcctatactttctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGa                                                                                                                                                                                     >  1:29883S26‑M2/184‑200 (MQ=255)                     aaccttggtcacggcattcatcaggatgtgccgccagaacatgctggcgtgttcgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatactttctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGA‑‑‑‑GGATAAtt                                                                                                                                                                                >  1:26951S29‑M2/179‑200 (MQ=255)                      accttggtcacggcattcatcaggatgtgccgccagaacatgctggcgtgttcgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatactttctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAAttt                                                                                                                                                                               >  1:4193S46‑M2/178‑200 (MQ=255)                            tcacggcattcatcaggatgtgccgccagaacatgctggcgtgttcgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatacttttctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTAt                                                                                                                                                                         >  1:31892S58‑M2/172‑200 (MQ=255)                                acggcattcatcaggatgtgccgccagaacatgctggcgtgttcgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatacttttagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑ga                                                                                                                                                                    >  1:18012S10‑M2/168‑200 (MQ=255)                                   gcattcatcaggatgtgccgccagaacatgctggcgtgttcgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatctttctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGaa                                                                                                                                                                 >  1:20808S17‑M2/165‑200 (MQ=255)                                       tcatcaggatgtgccgccagaacatgctggcgtgttcgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatactttctgagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑Ca                                                                                                                                                            >  1:32217S55‑M2/161‑200 (MQ=255)                                         tcaggatgtgccgccagaacatgctggcgtgttcgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatactttctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAAc                                                                                                                                                          <  1:2096S4‑M2/42‑1 (MQ=255)                                                tgtgccgccagaacatgctggcgtgttcgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatactttctagaatagggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTAc                                                                                                                                                   <  1:30126S70‑M2/49‑1 (MQ=255)                                                 tgccgccagaacatgctggcgtgttcgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatactttctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACa                                                                                                                                                  >  1:13063S53‑M2/151‑200 (MQ=255)                                                              tgctggcgtgttcgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatactttctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GA‑AAC‑‑CAACATGTTACAAAACCCAATTCATCt                                                                                                                                   <  1:551S55‑M2/63‑1 (MQ=255)                                                              gctggcgtgttcgtggaggcagtgcatcgactgtctgaaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatactttctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑‑GACATGTTACAAAACCCAATTCATc                                                                                                                                    <  1:11998S30‑M2/63‑1 (MQ=255)                                                                 tggcgtgttcgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatactttctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACAAAACCCAATTCATCTg                                                                                                                                  >  1:30468S105‑M2/135‑200 (MQ=255)                                                                          cgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattcaagcggccgccaccgcgggaagttcctatactttctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACAAAACCCAATTCATCTGCGTCTGGAg                                                                                                                         >  1:16858S7‑M2/126‑200 (MQ=255)                                                                            gttcgtggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggagttctgactccagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACAAAACCCAATTCATCTGCGTCTGGAgcg                                                                                                                       <  1:30468S34‑M2/77‑1 (MQ=255)                                                                            ggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctttttatactttctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACAAAACCCAATTCATCTGCGTCTGGAgcg                                                                                                                       >  1:26250S6‑M2/124‑200 (MQ=255)                                                                              tggaggcagtgcatcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctagtactctcagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACAAAACCCAATTCATCTGCGTCTGGAGCGcc                                                                                                                     <  1:14569S15‑M2/79‑1 (MQ=255)                                                                                       catcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatactttctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACAAAACCCAATTCATCTGCGTCTGGAGCGCCT‑GAAAGCTg                                                                                                           <  1:16421S6‑M2/88‑1 (MQ=255)                                                                                        atcgactgtctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatactttctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTAGAAAACCCAATTCATCTGCGTCTGGAGCGCCTGGAAAGCTg                                                                                                           <  1:22175S25‑M2/89‑1 (MQ=255)                                                                                                tctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatactttctagagaataggaagcctcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTTAAAAAACTGGAGCATG‑TACCAAACCCAATTCATCTGCGTCTGGAGCGCCTGGaaagctgggccaca                                                                                                    >  1:14568S2‑M2/104‑194 (MQ=255)                                                                                                tctgaacagtatcaccgctaaggagtgattagagcggccgccaccgcgggaagttcctatactttctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACAAAACCCAATTCATCTGCGTCTGGAGCGCCTGG‑‑AGCT‑GCAGCATGTCa                                                                                                <  1:26255S4‑M2/97‑1 (MQ=255)                                                                                                            accgctaaggagtgattagagcggccgccaccgcggggagttctgatactttctcagagaataggaaccttcgcatgcacgcagcatatgcTTCAGGGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACAAAACCCAATTCATCTGCGTCTGGAGCGCCTGGAAAGCTGGCAGCATGTCACTTTCATg                                                                                        <  1:1195S32‑M2/109‑1 (MQ=255)                                                                                                                                agcggccgccaccgcgggaagttcctatactttctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACAAAACCCAATTCATCTGCGTCTGGAGCGCCTGGAAAGCTGGCAGCATGTCACTTTCATGGCTTGCTTATG‑CGAACGCATg                                                                  <  1:4794S40‑M2/129‑1 (MQ=255)                                                                                                                                    ggccgccaccgcgggaagttcctatactttctggatgacggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACAAAACCCAATTCATCTGCGTCTGGAGCGCCTGGAAAGCTGGCAGCATGTCTCGCTGATCGCTTGCTTATG‑CGAACGCATGTAcc                                                              >  1:27946S15‑M2/68‑200 (MQ=255)                                                                                                                                       gccaccgccgggaagttcctatactttctgagatacggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACAAAACCCAATTCATCTGCGTCTGGAGCGCCTGGAAAGCTGGCAGCATGTCACTTTCATGGCTTGCTTATG‑CGAACGCATGTACCCCa                                                           >  1:22427S95‑M2/65‑200 (MQ=255)                                                                                                                                                    gttcctatactttctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGGGATC‑CAGAACT‑‑‑ACATGTTACAAAACCCAATTCATCTGCGTCTGGAGTGCCTGGAAAGCTGGCAGCATGTCACTTTCATGGCTTGCTTATG‑CGAACGCATGTACCCCAATTACGCCATGTTCt                                            <  1:1479S10‑M2/149‑1 (MQ=255)                                                                                                                                                     gttcctatactttctcaagaatagggaaggcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACAAAACCCAATTCATCTGCGTCTGGAGCGCCTGGAAAGCTGGCAGCATGTCACTTTCATG‑‑‑‑GCTTATGCCGAACGTATGTACCCCAATTACGCCATGTTCTGc                                          >  1:29000S21‑M2/51‑200 (MQ=255)                                                                                                                                                                tctagagaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACAAAACCCAATTCATCTGCGTCTGGAGCGCCTGGAAAGCTGGCAGCATGTCACTTTCATGGCTTGCTTATG‑CGAACACATG‑‑‑CCCAATTACGCCATGTTCTGCCAGCAAACCgg                               >  1:7595S12‑M2/40‑200 (MQ=255)                                                                                                                                                                tctagacaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGCTGGTGGATAATTTCCGTATTT‑GAGACCC‑TAACATGTTACAAAACCCAATTCATCTGTGTCTGGAGCGCCTGGAAAGCTGGCAGCATGTCACTTTCATGGCTTGCTTATG‑CGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGc                                      <  1:7591S92‑M2/161‑1 (MQ=255)                                                                                                                                                                      gaataggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACAAAACCCAATTCATCTGCGTCTGGAGCGCCTGGAAAGCTGGCAGCATGTCACTTTCATGGCTTGCTTATG‑CGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGttt                            <  1:18514S54‑M2/167‑1 (MQ=255)                                                                                                                                                                         taggaaccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACAAAACCCAATTCATCTGCGTCTGGAGCGCCTGGAAAGCTGGCAGCATGTCACTTTCATGGCTTGCTTATG‑CGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTgg                         <  1:22336S12‑M2/170‑1 (MQ=255)                                                                                                                                                                               ccttcgcatgcacgcagcatatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGGA‑TC‑CAGAACT‑CAACATGTTACAAAACCCAATTCATCTGCGTCTGGAGCGCCTGGAAAGCTGGCAGCATGTCACTTTCATGGCTTGCTTATG‑CGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTggtgacggc                  <  1:477S11‑M2/176‑5 (MQ=255)                                                                                                                                                                                                catatgcTTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACAAAACCCAATTCATCTGCGTCTGGAGCGCCTGGAAAGCTGGCAGCATGTCACTTTCATGGCTTGCTTATG‑CGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCa  <  1:2485S89‑M2/193‑1 (MQ=255)                                                                                                                                                                                                       |                                                                                                                                                                                                         CTGGCCTGGGTCTGGCGCAGCAAAGCGCGCTGTAACCCGTTGTTTATCGCTACCGGCCATCGGGTCAGCGTGGACAGCGCGCTGGCGTGGGTACAACGCTGCATGAAAGGCTATCGTCTGCCGGAGCCAACGCGCTGGGCGGACGCGGTGGCCTCGGAACGTCCGGCGTTCGTGCGCTATACAGCAAATCAGCCCTAATTCA‑GGTAAACTGC‑‑‑‑GGATAATTTCCGTATTT‑GAGAACT‑CAACATGTTACAAAACCCAATTCATCTGCGTCTGGAGCGCCTGGAAAGCTGGCAGCATGTCACTTTCATGGCTTGCTTATG‑CGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCA  >  NC_010473/4296983‑4297373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 47 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑