Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_010473	2,806,542	689 bp→GAGCGGCCGCCACCGCGGGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGCATGCACGCAGCATATGC	coding (2‑690/690 nt)	ung →	uracil‑DNA glycosylase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_010473	2806542	2807230	689	21 [0]	[0] 19	ung	uracil‑DNA glycosylase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_010473	= 2806541	0 (0.000)	15 (0.610) +GAGCGGCCGCCACCGCGGGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGCATGCACGCAGCATATGC	13/256	1.2	100%	coding (1/690 nt)	ung	uracil‑DNA glycosylase
	?	NC_010473	2807231 =	0 (0.000)					intergenic (+1/+47)	ung/yfiF	uracil‑DNA glycosylase/tRNA/rRNA methyltransferase

TCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGTACTGAAAGCACCGCATCCGTCGCCGCTTTCGGCGCATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGC‑GGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCCCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATC  >  NC_010473/2807036‑2807403                                                                                                                                                                                                    |                                                                                                                                                                              gggtgtgaaattttaccaatttacatttttttgcactcgtttaagtctaaaaaatgagcactattttgttctgtagaaagaagcagttaagctaggcggattgaagattcgcaggagagcgagagagcggccgccaccgcgggaagttcctatactttctagagaataggaacttcgcatgcacgcagcatatgcATTTg                                                                                                                                                                           <  1:12743S67‑M2/5‑1 (MQ=255)       tttaccaatttacatttttttgcactcgtttaagtctaaaaaatgagcatgatttgtttttcttgtagaaagaagcagttaagctaggcggattgaagattcgcaggagagcgagagagcggccgccaccgcgggagtttctctgatactttctagagaataggaacttcgcatgcacgcagcatatgcATTtgc‑ggtaa                                                                                                                                                                    <  1:10546S61‑M2/11‑4 (MQ=255)            tttaccaatttacatttttttgcactcgtttaagtctaaaaaatgagcatgattttgttctgtagaaagaagcagttaagctaggcggattgaagattcgcaggagagcgagagagcggccgccaccgcgggaagttcctatactttctagagaataggaacttcgcatgcacgcagcatatgcATTTGC‑GGGGAAATGc                                                                                                                                                               >  1:1907S78‑M2/185‑200 (MQ=255)             ttaccaatttacatttttttgcactcgtttaagtctaaaaaatgagcatgattttgttctgtagaaagaagcagttaagctaggcggattgaagattcgcaggagagcgagagagcggccgccaccgcgggaagttcctatactttctagagaataggaacttcgcatgcacgcagcatatgcATTTGC‑GGGAAAATGcc                                                                                                                                                              <  1:32087S78‑M2/17‑1 (MQ=255)                       acatttttttgcactcgtttaagtctaaaaaatgagcatgattttgttctgtagaaagaagcagttaagctaggcggattgaagattcgcaggagagcgagagagcggccgccaccgcgggaagttcctatactttctagagaataggaacttcgcatgcacgcagcatatgcatttgc‑ggcatgcccggatggcagagt                                                                                                                                                    <  1:14235S5‑M2/27‑20 (MQ=255)                                   cactcgtttaagtctaaaaaatgagcatgattttgttctgtagaaagaagcagttaagctaggcggattgaagattcgcaggagagcgagagagcggccgccaccgcgggaagttctatactttctagagaataggaacttcgcatgcacgcagcatatgcATTTGC‑GGGGAAATGCCGGATGGCAGAGTTGCCACCCgg                                                                                                                                        >  1:5567S2‑M2/162‑200 (MQ=255)                                       gtttaagtctaaaaaatgagcatgattttgttctgtagaaagaagcagttaagctaggcggattgaagattcgcaggagagcgagagagcggccgccaccgcgggaagttcctatactttctagagaataggaacttcgcatgcacgcagcatatgcATTTGCGGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTg                                                                                                                                     <  1:2149S1‑M2/43‑1 (MQ=255)                                                                     ttctgtagaaagaagcagttaagctaggcggattgaagattcgcaggagagcgagagagcggccgccaccgcgggaagttcctatactttctagagaataggaacttcgcatgcacgcagcatatgcATTTGC‑GGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCAtt                                                                                                      <  1:3103S50‑M2/73‑1 (MQ=255)                                                                       tgtagaaagaagcagttaagctaggcggattgagagattcgcaggagagcgagagagcggccgccaccgcgggaagttcctatactttctagagaataggaacttcgcatgcacgcagcatatgcATTTGC‑GGGGAAATGCCGGATGGCA‑AACTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCAc                                                                                                   <  1:9708S93‑M2/75‑1 (MQ=255)                                                                             aagaagcagttaagctaggcggattgaagattcgcaggagagcgagagagctggccgccaccgcgggaagttcctatactttctagagaataggaacttcgcatgcacgcagcatatgcATTTGC‑GGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGc                                                                                              <  1:1894S17‑M2/81‑1 (MQ=255)                                                                                   aagaagcagttaagctaggcggattgaagattcgcaggagagcgagagagcggccgccaccgcgggaagttcctatactttctaaatagaactcgcatgcacgcagcatatgcATTTGC‑GGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACg                                                                                        >  1:23878S24‑M2/114‑200 (MQ=255)                                                                                     agttaagctaggcggattgaagattcgcaggagagcgagagagcggccgccaccgcgggaagttcctatactttctagagaataggaacttcgcatgcacgcagcatatgcATTTGC‑GGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGcc                                                                                      <  1:18994S60‑M2/89‑1 (MQ=255)                                                                                               ggcggattgaagattcgcaggagagcgagagagcggccgccaccgcgggaagttcctatactttctagagaataggaacttcgcgtgcacgcagcatatgcATTTGC‑GGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACa                                                                            >  1:723S1‑M2/102‑200 (MQ=255)                                                                                                  aggcggattgaagattcgcaggagagcgagagagcggccgccaccgcgggaagttcctatactttctagagaataggaacttcgcataggcatattgcGTTTGC‑GGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACagag                                                                         >  1:14233S10‑M2/99‑200 (MQ=255)                                                                                                                   aggagagcgagagagcggccgccaccgcgggagttcaatactttctagagaataggaacttcgcatgcacgcagcatatgcATTTGC‑GGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAAc                                                        <  1:29180S16‑M2/119‑1 (MQ=255)                                                                                                                                        caccgcggaagttcctatactttctagagaataggaacttcgcatgcacgcagcatatgcATTTGC‑GGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCCCGTACCGTCAATCtt                                   <  1:6781S6‑M2/140‑1 (MQ=255)                                                                                                                                                aagttcctatactttctagagaataggaacttcgcatgcacgcagcatatgcATTTGC‑GGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCCCGTACCGTCAATCTTCACGCGCa                           <  1:27835S206‑M2/148‑1 (MQ=255)                                                                                                                                                                 agagaataggaacttcgcatgcacgcagcatatgcATTTGC‑GGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCCCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGt          >  1:24699S7‑M2/36‑200 (MQ=255)                                                                                                                                                                         ggaacttcgcatgcacgcagcatatgcATTTGC‑GGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCCCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATc  >  1:12855S15‑M2/28‑200 (MQ=255)                                                                                                                                                                                                    |                                                                                                                                                                              TCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGTACTGAAAGCACCGCATCCGTCGCCGCTTTCGGCGCATCGTGGATTCTTTGGCTGCAACCATTTTGTGCTGGCAAATCAGTGGCTGGAACAACGTGGCGAGACGCCGATTGACTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGC‑GGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCCCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATC  >  NC_010473/2807036‑2807403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 48 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑