This year, we introduced several new basic parts to the iGEM registry and designed our own constructs to support our project. On this page, you’ll find the new parts we’ve added, along with brief descriptions of each. We’ve also categorised all the constructs we developed, including their individual parts and explanations of their intended functions. For more details on how our constructs performed, feel free to explore our design and engineering pages!
New Basic Parts
| Pathway |
Part |
Name |
Length (bp) |
Description |
| Magnetic |
BBa_K5154001 |
EPG |
396 |
Electromagnetic Perceptive Gene with signal peptides |
| Magnetic |
BBa_K5154002 |
EPG_NanoLuc |
945 |
EPG-Split Nanoluciferase fusion protein |
| Magnetic |
BBa_K5154003 |
N-TEV |
358 |
N-terminal fragment of TEV protease (1-119), adapted from BBa_J435001 |
| Magnetic |
BBa_K5154004 |
C-TEV |
382 |
C-terminal fragment of TEV protease (120-237), adapted from BBa_J435001 |
| Magnetic |
BBa_K5154006 |
TEV-F5-EPG |
1158 |
EPG-Split TEV fusion with flexible 5 amino acid linker |
| Magnetic |
BBa_K5154005 |
TEV-R5-EPG |
1158 |
EPG-Split TEV fusion with rigid 5 amino acid linker |
| Mechanical |
BBa_K5154015 |
Elongated Wsc1 |
1695 |
Wsc1 fused with Mid2, and with His8 tag inserted between secretion signal sequence |
| Mechanical |
BBa_K5154016 |
LexA-Rlm1 |
1982 |
LexA fused to two thirds of Rlm1 |
| Mechanical |
BBa_K5154000 |
Galp-lexAops |
745 |
Synthetic Gal promoter with the UAS replaced by a LexA binding site |
New Composite Parts
| Pathway |
Part |
Name |
Length (bp) |
Description |
Sequence |
| Magnetic |
BBa_K5154007 |
T7TE_pTi_T7LacO_BN1BCD2_NlucEPG_T7Term |
5839 |
Expression plasmid for EPG Nanoluc. High strength. Kanamycin Resistant. |
Download |
| Magnetic |
BBa_K5154008 |
TEV_Rep-pSB1C5C_J23119_BCD2HisFuGFP_TEV_REACh2_B0015 |
3806 |
Expression plasmid for fuGFP-REACh2 protein. Act as reporter for TEV activity. Chloramphenicol resistant |
Download |
| Magnetic |
BBa_K5154009 |
F5EPG-pTi_J23109_BN1BCD2_TEVNF5_EPG_CF5_B0015 |
6058 |
Expression plasmid for EPG-TEV Fusion with Flexible 5aa linker. Kanamycin Resistant. |
Download |
| Magnetic |
BBa_K5154010 |
TE_pTi_J23100_BN1BCD2_NlucEPG_B0015 |
5837 |
Expression plasmid for EPG Nanoluc. Medium strength. Kanamycin Resistant. |
Download |
| Magnetic |
BBa_K5154011 |
CE_pSB1C5C-EPGNLuc |
2972 |
Cloning plasmid for EPG-NLuc construct. |
Download |
| Magnetic |
BBa_K5154012 |
LE- pSB1C5CLin_J23100_BN1BCD2_NLucEPG_B0015 |
3270 |
Expression plasmid for EPG Nanoluc. Medium strength. Chloramphenicol Resistant. |
Download |
| Magnetic |
BBa_K5154013 |
TR-pTi_J23100_BN1BCD2_Renilla_B0015 |
5836 |
Expression plasmid for Renilla Luciferase. Positive control to Nanoluciferase.Medium strength. Kanamycin Resistant. |
Download |
| Magnetic |
BBa_K5154014 |
R5EPG-pTi_J23109_BN1BCD2_TEVNR5_EPG_CR5_B0015 |
6060 |
Expression plasmid for EPG-TEV Fusion with rigid5aa linker. Kanamycin Resistant. |
Download |
Improvement on an existing part
We also constructed a double-plasmid system based on an existing biopart TcI BBa_K2969021. The successful double-plasmid system will allow us to replace the fluorescent protein with any cds, and the expression of the selected cds will be strictly heat-sensitive.
| Pathway |
Part |
Name |
Length (bp) |
Description |
Sequence |
| Thermal |
BBa_K2969021 |
TcI gene expression (1p V1 and V2) |
2702 |
Plasmid expressing the TcI protein. |
Download |
| Thermal |
BBa_K2969021 |
TcI-dependent fluorescent protein expression (2p V1) |
2669 |
Plasmid that expresses mRFP1 when not repressed by TcI. |
Download |