Protocal English Version
We meticulously documented our entire experimental process and created an experimental manual to help future iGEM teams and researchers replicate or build on our work. The manual is openly accessible and provides step-by-step instructions for constructing bioluminescent plants with both English and Chinese versions.
Content
- Cloning PCR: Page 1
- Enzyme Digestion Reaction: Page 2
- Agarose Gel Electrophoresis: Pages 3 to 4
- Product Purification: Page 5
- Homologous Recombination Ligation Reaction: Page 6
- Transformation into Competent E. coli: Page 7
- Transformation into Competent Agrobacterium: Page 8
- Single Colony Picking: Page 9
- Positive Single Colony Liquid PCR Verification: Page 10
- Sequencing Comparison: Page 11
- Plasmid Extraction: Pages 12 to 13
- Tobacco Cultivation Manual: Pages 14 to 16
- Plant RNA Extraction: Pages 17 to 19
- Reverse Transcription Experiment: Page 20
- Plant DNA Extraction: Pages 21 to 23
- Transient Transformation Verification and GUS Staining of Tobacco Leaves: Pages 24 to 25
Protocal Chinese Version
