1. Throughout the project, no high-risk biological samples were used. The DH5α and EcN1917 Escherichia coli strains were handled carefully within a fume hood, following established operational protocols. All cultivation processes were conducted under aseptic conditions to prevent cross-contamination.

2.Waste was treated with chemical disinfectants, specifically sodium hypochlorite solution, and all resulting wastewater was collected and disposed of according to the laboratory's regulations.

3.All containers used for culturing the strains were clearly labeled with the strain name, culture date, and operator’s name to prevent any potential confusion or misidentification.

1.Our instructor provided comprehensive safety training in the laboratory, covering the recognition of laboratory safety signs, safety equipment, and the appropriate dress code for lab work.

2.Laboratory coats must be worn exclusively within the lab and removed when entering living areas. Additionally, gloves are required when handling materials in the lab.

3.After using laboratory materials, waste should be properly sorted into the designated classification bins as instructed.

4.All laboratory waste is centrally processed at the ATL lab center, ensuring that it is handled in a way that prevents any pollution or harm to the environment.