Colorectal cancer is a leading cause of cancer-related mortality worldwide. This project focuses on developing a novel targeted therapy by inhibiting the TEAD4 gene, a key regulator in the Hippo signaling pathway that plays a significant role in colorectal cancer progression. We have designed two plasmids that efficiently suppress TEAD4 expression. Our results indicate that elevated levels of TEAD4-targeting siRNAs in the colorectal cancer cell line SW480 lead to inhibition of tumor cell proliferation and migration, as well as increased reactive oxygen species (ROS) expression. These findings suggest that this RNA interference vector could serve as a promising therapeutic candidate for colorectal cancer treatment.