. Parts .
1.Overview
This year, among the 93 parts designed, 44 of them are basic parts and the rest are composite parts. All
of them are designed by ourselves, and most of them have been tested to insure their reliability. You can
click here
to learn more about our project to see their specific functions, or click on the part numbers
when browse website.
2.Basic Parts
Among the 44 basic parts designed this year, BBa_K4907016 is our favorite one that codes VSW-3 RNA
Polymerase (VSW-3 RNAP). This gene not only realizes our AND gate design successfully, but also shows
particular talent in low-temperature expression systems. This excellent coding sequence companied with its
corresponding promoters will be a novel and prospective tool for other researchers and teams. Besides, many
other basic parts have also been designed to construct our project.
| Favorite | Name | Type | Description |
|---|---|---|---|
| BBa_K3512001 | coding | ccdB | |
| BBa_K4907032 | coding | ccdA | |
| BBa_I0500 | regulatory | I0500 | |
| BBa_B0034 | RBS | B0034 | |
| BBa_B0015 | regulatory | B0015 | |
| BBa_J23100 | regulatory | J23100 | |
| BBa_J23106 | regulatory | J23106 | |
| BBa_B0014 | regulatory | B0014 | |
| BBa_K5136000 | coding | CYP199A4 WT-his tag （1） | |
| BBa_K5136001 | coding | CYP199A4 T253E-his tag | |
| BBa_K5136002 | coding | CYP199A4 T253S-his tag | |
| BBa_K5136003 | coding | CYP199A4 T253A-his tag | |
| BBa_K5136004 | coding | CYP199A4 T253R-his tag | |
| BBa_K5136005 | coding | CYP199A4 T253N-his tag | |
| BBa_K5136006 | coding | CYP199A4 T253D-his tag | |
| BBa_K5136007 | coding | CYP199A4 T253C-his tag | |
| BBa_K5136008 | coding | CYP199A4 T253Q-his tag | |
| BBa_K5136009 | coding | CYP199A4 T253W-his tag | |
| BBa_K5136010 | coding | CYP199A4 T253G-his tag | |
| BBa_K5136011 | coding | CYP199A4 T253H-his tag | |
| BBa_K5136012 | coding | CYP199A4 T253I-his tag | |
| BBa_K5136013 | coding | CYP199A4 T253L-his tag | |
| BBa_K5136014 | coding | CYP199A4 T253K-his tag | |
| BBa_K5136015 | coding | CYP199A4 T253M-his tag | |
| BBa_K5136016 | coding | CYP199A4 T253F-his tag | |
| BBa_K5136017 | coding | CYP199A4 T253P-his tag | |
| BBa_K5136018 | coding | CYP199A4 T253V-his tag | |
| BBa_K5136019 | coding | CYP199A4 T253Y-his tag | |
| BBa_K5136020 | coding | SfmD WT | |
| BBa_K5136021 | coding | SfmD 277F | |
| BBa_K5136022 | coding | TyrH-histag | |
| BBa_K5136023 | coding | OleTJE | |
| BBa_K5136024 | coding | OleTJE 236ser 100lys 166lys | |
| BBa_K5136025 | coding | OleTJE 236ser 360lys 167lys | |
| BBa_K5136026 | coding | cellulase-histag | |
| BBa_K5136027 | coding | laccase-histag | |
| BBa_K5136028 | coding | superfolder GFP(sfgfp) | |
| BBa_K5136029 | coding | FhuD-TSS linker-T7 lysozyme 119V-SsrA | |
| BBa_K5136030 | coding | FhuD-TSS linker-T7 lysozyme 119G-SsrA | |
| BBa_K5136031 | coding | FhuD-GGG linker-T7 lysozyme 119V-SsrA | |
| BBa_K5136032 | coding | FhuD-GGG linker-T7 lysozyme 119G-SsrA | |
| BBa_K5136033 | coding | LMT - GGG linker-T7 lysozyme 119G-SsrA | |
| BBa_K5136034 | coding | histag-spytag-gfpFasta | |
| BBa_K5136035 | coding | inpnc-histag-spycatcher | |
| BBa_K5136036 | coding | mt3-linker | |
| BBa_K5136037 | coding | mt3 | |
| BBa_K5136038 | coding | mt2a | |
| BBa_K5136039 | coding | mnta | |
| BBa_K5136040 | coding | gst-linker | |
| BBa_K5136041 | coding | crs5 | |
| BBa_K5136042 | coding | LexRO | |
| BBa_K5136043 | regulatory | pHybrid 2)-114 version | |
| BBa_K5136044 | regulatory | pColE408-RBS | |
| BBa_K5136045 | RBS | SD7 | |
| BBa_K5136046 | plasmid | pSB4A5 (with pColE408-aca2) | |
| BBa_K5136047 | regulatory | RiboJ | |
| BBa_K5136048 | coding | inpnc-linker | |
| BBa_K5136050 | coding | Algen-his tag-linker-sfgfp | |
| BBa_K5136051 | coding | Aly01-his tag-linker-sfgfp | |
| BBa_K5136052 | coding | OmpA-his tag-linker-sfgfp | |
| BBa_K5136053 | coding | PelB-his tag-linker-sfgfp | |
| BBa_K5136054 | coding | TorA-his tag-linker-sfgfp | |
| BBa_K5136055 | coding | YebF-his tag-linker-sfgfp | |
| BBa_K5136056 | coding | LMT-his tag-linker-sfgfp | |
| BBa_K5136057 | coding | rfbD | |
| BBa_K5136058 | coding | rhlA | |
| BBa_K5136059 | coding | rhlB |
3.Composite Parts
We have designed a series of composite parts this year, and most of them could be useful tools for our
design. And our favorite composite part is BBa_K4907114,
which contains the sequence of VSW-3 RNAP to
activate target genes' expression.
| Favorite | Name | Type | Description |
|---|---|---|---|
|
BBa_K4907114 | Composite | I0500-B0034-VSW-3 rnap-B0015 |
| BBa_K5136200 | composite | J23100-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136201 | composite | J23101-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136202 | composite | J23102-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136203 | composite | J23103-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136204 | composite | J23104-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136205 | composite | J23105-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136206 | composite | J23106-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136207 | composite | J23107-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136208 | composite | J23108-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136209 | composite | J23109-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136210 | composite | J23110-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136211 | composite | J23111-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136212 | composite | J23113-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136213 | composite | J23114-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136214 | composite | J23115-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136215 | composite | J23116-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136216 | composite | J23117-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136217 | composite | J23118-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136218 | composite | J23119-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136219 | composite | I05000-B0034-FhuD-TSS linker-T7 lysozyme 119V-SsrA-B0015 | |
| BBa_K5136220 | composite | I05000-B0034-FhuD-TSS linker-T7 lysozyme 119G-SsrA-B0015 | |
| BBa_K5136221 | composite | I05000-B0034-FhuD-GGG linker-T7 lysozyme 119V-SsrA-B0015 | |
| BBa_K5136222 | composite | I05000-B0034-FhuD-GGG linker-T7 lysozyme 119G-SsrA-B0015 | |
| BBa_K5136223 | composite | I05000-B0034-LMT-GGG linker-T7 lysozyme 119G-SsrA-B0015 | |
| BBa_K5136224 | composite | I05000-B0034-inpnc-histag-spycatcher-B0015 | |
| BBa_K5136225 | composite | I05000-B0034-mnta-B0015 | |
| BBa_K5136226 | composite | I05000-B0034-inpnc-linker-mt3-linker-mt2a-B0015 | |
| BBa_K5136227 | composite | I0500-B0034-gst-linker-mt3-B0015 | |
| BBa_K5136228 | composite | I0500-B0034-gst-linker-mt2a-B0015 | |
| BBa_K5136229 | composite | I0500-B0034-gst-linker-crs5-B0015 | |
| BBa_K5136230 | composite | I0500-B0034-mt3-B0034-mt2a-B0015 | |
| BBa_K5136231 | composite | J23106-SD7-LexRO-B0015-pColE408-ccdB-B0014-pHybrid 2)-114 version-B0034-ccdA | |
| BBa_K5136232 | composite | I0500-B0034-rhlA-B0034-rhlB-B0015 | |
| BBa_K5136233 | composite | J23100-rfbD-B0015 | |
| BBa_K1460002 | nan | nan |
