. Parts .
Overview
This year, among the 89 parts designed, 65 of them are basic parts and the rest are composite parts. All
of them are designed by ourselves, and most of them have been tested to insure their reliability. You can
click here to learn more about our project to see their
specific functions, or click on the part numbers when browse website.
Basic Parts
Among the 65 basic parts designed this year, BBa_K5136003 is our favorite one that codes CYP199A4
T253A-His tag. Among all of monooxygenases we tested, the monooxygenase encoded by this gene has the
best
deinking effect. This monooxygenase can exhibit
excellent performance in related research conducted by other researchers and iGEM teams. Besides, many other
basic parts have also been designed to construct our project.
| Favorite | Name | Type | Description |
|---|---|---|---|
| BBa_K5136000 | coding | CYP199A4 WT-His tag | |
| BBa_K5136001 | coding | CYP199A4 T253E-His tag | |
| BBa_K5136002 | coding | CYP199A4 T253S-His tag | |
|
BBa_K5136003 | coding | CYP199A4 T253A-His tag |
| BBa_K5136004 | coding | CYP199A4 T253R-His tag | |
| BBa_K5136005 | coding | CYP199A4 T253N-His tag | |
| BBa_K5136006 | coding | CYP199A4 T253D-His tag | |
| BBa_K5136007 | coding | CYP199A4 T253C-His tag | |
| BBa_K5136008 | coding | CYP199A4 T253Q-His tag | |
| BBa_K5136009 | coding | CYP199A4 T253W-His tag | |
| BBa_K5136010 | coding | CYP199A4 T253G-His tag | |
| BBa_K5136011 | coding | CYP199A4 T253H-His tag | |
| BBa_K5136012 | coding | CYP199A4 T253I-His tag | |
| BBa_K5136013 | coding | CYP199A4 T253L-His tag | |
| BBa_K5136014 | coding | CYP199A4 T253K-His tag | |
| BBa_K5136015 | coding | CYP199A4 T253M-His tag | |
| BBa_K5136016 | coding | CYP199A4 T253F-His tag | |
| BBa_K5136017 | coding | CYP199A4 T253P-His tag | |
| BBa_K5136018 | coding | CYP199A4 T253V-His tag | |
| BBa_K5136019 | coding | CYP199A4 T253Y-His tag | |
| BBa_K5136020 | coding | SfmD WT | |
| BBa_K5136021 | coding | SfmD 277F | |
| BBa_K5136023 | coding | OleTJE | |
| BBa_K5136024 | coding | OleTJE 236S 100K 166K | |
| BBa_K5136025 | coding | OleTJE 236S 360K 167K | |
| BBa_K5136026 | coding | cellulase EG5C-His tag | |
| BBa_K5136027 | coding | laccase-His tag | |
| BBa_K5136028 | coding | sfgfp | |
| BBa_K5136029 | coding | FhuD-TSS linker-T7 lysozyme 119V-SsrA | |
| BBa_K5136030 | coding | FhuD-TSS linker-T7 lysozyme 119G-SsrA | |
| BBa_K5136031 | coding | FhuD-GGG linker-T7 lysozyme 119V-SsrA | |
| BBa_K5136032 | coding | FhuD-GGG linker-T7 lysozyme 119G-SsrA | |
| BBa_K5136033 | coding | LMT-GGG linker-T7 lysozyme 119G-SsrA | |
| BBa_K5136034 | coding | His tag-SpyTag-gfp | |
| BBa_K5136035 | coding | inpnc-His tag-SpyCatcher | |
| BBa_K5136037 | coding | mt3 | |
| BBa_K5136038 | coding | mt2a | |
| BBa_K5136039 | coding | mnta | |
| BBa_K5136040 | coding | gst-linker-mt2a | |
| BBa_K5136041 | coding | gst-linker-crs5 | |
| BBa_K5136042 | coding | LexRO | |
| BBa_K5136043 | regulatory | pHybrid 2)-114 version | |
| BBa_K5136044 | regulatory | pColE408-RBS | |
| BBa_K5136045 | RBS | SD7 | |
| BBa_K5136047 | coding | LMT-linker-CYP199A4 T253E | |
| BBa_K5136048 | coding | inpnc-linker-mt3-linker-mt2a | |
| BBa_K5136049 | RBS | SD17 | |
| BBa_K5136050 | coding | Algen-His tag-linker-sfgfp | |
| BBa_K5136051 | coding | OsmY-His tag-linker-sfgfp | |
| BBa_K5136052 | coding | OmpA-His tag-linker-sfgfp | |
| BBa_K5136053 | coding | PelB-His tag-linker-sfgfp | |
| BBa_K5136054 | coding | TorA-His tag-linker-sfgfp | |
| BBa_K5136055 | coding | YebF-His tag-linker-sfgfp | |
| BBa_K5136056 | coding | LMT-His tag-linker-sfgfp | |
| BBa_K5136057 | coding | rfbD | |
| BBa_K5136058 | coding | rhlA | |
| BBa_K5136059 | coding | rhlB | |
| BBa_K5136060 | coding | Algen | |
| BBa_K5136061 | coding | OsmY | |
| BBa_K5136062 | coding | OmpA | |
| BBa_K5136063 | coding | PelB | |
| BBa_K5136064 | coding | TorA | |
| BBa_K5136065 | coding | YebF | |
| BBa_K5136066 | coding | LMT | |
| BBa_K5136067 | coding | HlyA |
Composite Parts
We have designed a series of composite parts this year, and most of them could be useful tools for our
design. Our favorite composite part is BBa_K5136202. This composite part demonstrates that the
combination
of the promoter J23104 and the signal peptide LMT can achieve the best secretion effect, providing strong
support for using signal peptide to obtain the deinking enzymes in our project.
| Favorite | Name | Type | Description |
|---|---|---|---|
| BBa_K5136200 | composite | J23100-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136201 | composite | J23103-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
|
|
BBa_K5136202 | composite | J23104-RiboJ-B0034-LMT-linker-sfgfp-B0010 |
| BBa_K5136203 | composite | J23106-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136204 | composite | J23110-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136205 | composite | J23114-RiboJ-B0034-LMT-linker-sfgfp-B0010 | |
| BBa_K5136219 | composite | I0500-B0034-FhuD-TSS linker-T7 lysozyme 119V-SsrA-B0015 | |
| BBa_K5136220 | composite | I0500-B0034-FhuD-TSS linker-T7 lysozyme 119G-SsrA-B0015 | |
| BBa_K5136221 | composite | I0500-B0034-FhuD-GGG linker-T7 lysozyme 119V-SsrA-B0015 | |
| BBa_K5136222 | composite | I0500-B0034-FhuD-GGG linker-T7 lysozyme 119G-SsrA-B0015 | |
| BBa_K5136223 | composite | I0500-B0034-LMT-GGG linker-T7 lysozyme 119G-SsrA-B0015 | |
| BBa_K5136224 | composite | I0500-B0034-inpnc-His tag-SpyCatcher-B0015 | |
| BBa_K5136225 | composite | I0500-B0034-mnta-B0015 | |
| BBa_K5136226 | composite | I0500-B0034-inpnc-linker-mt3-linker-mt2a-B0015 | |
| BBa_K5136228 | composite | I0500-B0034-gst-linker-mt2a-B0015 | |
| BBa_K5136229 | composite | I0500-B0034-gst-linker-crs5-B0015 | |
| BBa_K5136230 | composite | I0500-B0034-mt3-B0034-mt2a-B0015 | |
| BBa_K5136231 | composite | J23106-SD7-LexRO-B0015-pColE408-ccdB-B0014-pHybrid 2)-114 version-B0034-ccdA | |
| BBa_K5136232 | composite | I0500-B0034-rhlA-B0034-rhlB-B0015 | |
| BBa_K5136233 | composite | J23100-rfbD-B0015 | |
| BBa_K5136234 | composite | J23106-SD7-LexRO-B0015 | |
| BBa_K5136235 | composite | J23106-SD17-LexRO-pColE408-ccdB-pHybrid 2)-114 version-B0034-ccdA | |
| BBa_K5136236 | composite | I0500-B0034-ccdB-B0014-pHybrid 2)-114 version-B0034-ccdA | |
| BBa_K5136237 | composite | J23106-SD7-LexRO-B0015-pColE408-mCherry-B0015 |
