What is DHA ？

DHA is a long chain Omega-3 fatty acid that is a major structural component of the brain grey matter and retina.

According to the different structure of DHA, it can be divided into 3 types

Why do we produce PC-DHA ?

PC-DHA combines DHA with phospholipids, increasing bioavailability and maintaining lipid health, with the potential to cross the blood-brain barrier.

PC-DHA enhances the functional properties of DHA and has its own unique benefits ！

How can we obtain PC-DHA ?

PC-DHA
currently supplements are mainly krill oil, the raw material for which comes from Antarctic krill.
100

million tonnes of Antarctic krill are harvested each year.
183.2
million US dollars is the market size of krill oil.

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We found a SynBio solution :

We designed a biological production method that can be Eco-friendly, cost-effective, high-efficiency and high-purity.

So how does it work ?

We identified a pathway in Schizochytrium limacinum where DHA, CoA and ATP are substrates and LACS catalyses PC-DHA formation.

In the natural environment, the affinity of LACS for DHA is lower than that of even-carbon fatty acids

mRNA optimization

reaction mechanism study

mechanism-based mutagenesis

in-silico validation

in-vitro enzyme assay

We therefore mutated LACS using five modelling tools to obtain mutant sequences to improve its affinity for DHA.

Our experiment verified LACS's increase in DHA preference after optimization, proving it a better helper for PC-DHA manufature.