Part

BBa_K5474000

Our goal is to produce a double-stranded RNA (dsRNA) fungicide to prevent and control corn sheath blight caused by Rhizoctonia solani, which has led to a significant reduction in global corn production. We constructed a part, RsCAT-dsRNA (BBa_K5474000), to achieve this goal. The dsRNA was designed to silence the target gene RsCAT through the RNAi mechanism. Two T7 promoters are located on both sides of the dsRNA encoding sequence in opposite directions, so there is bidirectional transcription, which allows the RNA to form a double-stranded structure.

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