Florecent
Salmonella
Parts
“
Our project explores the groundbreaking potential of genetically engineered Salmonella in cancer therapy. By selectively targeting tumor cells and inducing apoptosis, we aim to create a more effective and less invasive treatment option.
Engineered Salmonella Beat Cancer
Overview
This year, we designed, built and characterized a set of parts. They were consisted of different parts collection and played different roles in different section of our parts. All the new parts had been submitted to The iGEM Parts Registry following the BioBricks assembly standard. On this page, we gave a brief description of our parts and we divided them into several sections according to the modules they worked in our project. For more detailed information about our parts, we highly recommend you to visit the corresponding parts page on The iGEM Parts Registry or turn to the Design and Engineering page of our wiki.
Basic Parts
Module Type Code Name Length Function and description
Killing System Protein Domain BBa_K5363000 Bax 628bp Bax protein promotes apoptosis by regulating mitochondrial membrane permeability, leading to the release of cytochrome c and other pro-apoptotic factors.
Killing System RNA BBa_K5363001 (MS2)24 1193bp A tandem repeats of RNA sequence from MS2 (Microbial Siphovirus 2) that can be recognized by MS2 coat protein (MCP).
Killing System Scar BBa_K5363002 HHR 76bp A cleavage site at the C terminal of MS2 repeats to remove the original poly-A tail of BAX mRNA.
Killing System Protein Domain BBa_K5363003 MCP 387bp MS2 coat protein, serving as one of the connection protein to form the translation loop.
Killing System Protein Domain BBa_K5363004 LaG16 810bp A high affinity antibody to EGFP, serving as one of the connection protein to form the translation loop.
Killing System Protein Domain BBa_K5363005 NS3a(H1) 810bp NS3a(H1) is a non-structural protein derived from the hepatitis C virus (HCV). It can bind to ANR protein, serving as one of the connection protein to form the translation loop.
Killing System Protein Domain BBa_K5363006 ANR-8 1047bp ANR, or Apo NS3a reader, is a genetically encoded peptide that binds to the active site of the apo form of NS3a(H1).
Killing System Protein Domain BBa_K5363007 NSP3 942bp NSP3 (Non-Structural Protein 3) is a multifunctional protein encoded by the coronavirus genome. It can bind to mRNA and eI4F proteins, serving as the connection protein to form the translation loop.
Reporter Protein Domain BBa_K5363011 EGFP 714bp EGFP (Enhanced Green Fluorescent Protein) can emit green fluorescence, which is much more greater than wild type GFP, when excited by light of a certain wavelength.
Reporter Protein Domain BBa_K5363020 mCherry 711bp MCherry is a red fluorescent protein from mushroom coral that emits red fluorescence when excited by light of a certain wavelength.
SLC Other BBa_K5363013 PsseJ promoter 368bp PsseJ promoter is a promoter that only activates after the host invades the cell.
SLC Protein Domain BBa_K5363015 LyseE 276bp LyseE is a protein that can induce host cell lysis.
SLC Other BBa_K5363018 Luxl promoter 151bp LuxI promoter is a common promoter that controls LuxI-LuxR system.
SLC Protein Domain BBa_K5363021 luxR 810bp LuxR is a protein that participates in regulation of target gene in LuxI-LuxR system.
Recognition System Protein Domain BBa_K5363023 sfGFP 714bp SfGFP is a fluorescent protein that is widely used as a reporter protein in molecular biology to visualize and track gene expression.
Recognition System Protein Domain BBa_K5363014 Lpp-OmpA-1 450bp Surface display system for Salmonella or E.coli to express proteins to the outer surface of the outer membrane.
Recognition System Protein Domain BBa_K5363016 Non-OmpA 399bp Surface display system for Salmonella or E.coli to express proteins to the outer surface of the outer membrane.
Recognition System Protein Domain BBa_K5363017 HER2-scFv 732bp HER2-scFv is a kind of antibody specifically recognises and binds to HER2, which is an overexpressed supramembrane receptor in most breast cancer cells.
Composite Parts
Module Type Code Name Length Function and description
Killing System Coding BBa_K5363012 NSP3-ANR_8 1997bp One of the fused connection proteins to form the translation loop.
Killing System Coding BBa_K5363008 Bax-MS2 boxes 1913bp The effector gene that can be regulated by Bio-switch and its expression can induce cell apoptosis.
Killing System Coding BBa_K5363009 MCP-LaG16 1203bp One of the fused connection proteins to form the translation loop.
Killing System Coding BBa_K5363010 EGFP-NS3a(H1) 1530bp Simulate the specific intracellular signal, and is the key component of the translational loop of the effector gene.
Recognition System Coding BBa_K5363022 Lpp-OmpA-1-scFv 1188bp Display the HER2 scFv to the surface of E.coli or Salmonella.
Recognition System Coding BBa_K5363024 Lpp-OmpA-2-scFv 1202bp Display the HER2 scFv to the surface of E.coli or Salmonella.
Recognition System Coding BBa_K5363025 Non-OmpA-scFv 1137bp Display the HER2 scFv to the surface of E.coli or Salmonella.
Delivery System Generator BBa_K5363026 PsseJ-mCherry 1085bp Make Salmonella emit a red fluorescent signal after entering cells.
Delivery System Generator BBa_K5363027 PsseJ-lyseE 650bp Make Salmonella self-lyse after entering cells.
SLC Plasmid BBa_K5363031 pTD103 5563bp Contain LuxI-LuxR system and sfGFP.
SLC Plasmid BBa_K5363032 pTD103-lyseE 427bp Keep the population of Salmonella under a maximal level.