Appendix

Supplementary data from our experiments.

1. Pictures from the first maturation assay

maturation_1_full
Figure A1.1: Compilation of all pictures documenting the color development of amilCP, amilCP green (1 and 2) and mRFP1 over the course of 30 h after initial oxygen exposure.

2. Absorbance spectra

abs_NNN157A
Figure A2.1: Absorbance spectrum of the NNN157 A mutant, which is Asp with the codon GAT.
abs_asp
Figure A2.2: Absorbance spectrum of the Asp157 mutant with the codon GAC.

3. Scatter plots from the superfold maturation assay run

Scatter plot of mRFP1-F
Figure A3.1: Scatter plot of mRFP1-F.
Scatter plot of mRFP1-E
Figure A3.2: Scatter plot of mRFP1-E.
Scatter plot of mRFP1-H
Figure A3.3: Scatter plot of mRFP1-H.
Scatter plot of amilCP green-E
Figure A3.4: Scatter plot of amilCP green-E.
Scatter plot of amilCP green-H
Figure A3.5: Scatter plot of amilCP green-H.
Scatter plot of sf1-green-2-1-F
Figure A3.6: Scatter plot of sf1-green-2-1-F.
Scatter plot of sf1-green-2-2-F
Figure A3.7: Scatter plot of sf1-green-2-2-F.
Scatter plot of sf2-6-H
Figure A3.8: Scatter plot of sf2-6-H.
Scatter plot of sf2-8-H
Figure A3.9: Scatter plot of sf2-8-H.

4. Scatter plots from the color mutation maturation assay run

Scatter plot of mRFP1-B
Figure A4.1: Scatter plot of mRFP1-B.
Scatter plot of mRFP1-A
Figure A4.2: Scatter plot of mRFP1-A.
Scatter plot of mRFP1-C
Figure A4.3: Scatter plot of mRFP1-C.
Scatter plot of mRFP1-D
Figure A4.4: Scatter plot of mRFP1-D.
Scatter plot of amilCP green-A
Figure A4.5: Scatter plot of amilCP green-A.
Scatter plot of amilCP green-D
Figure A4.6: Scatter plot of amilCP green-D.
Scatter plot of NNN157-B-C
Figure A4.7: Scatter plot of NNN157-B-C.
Scatter plot of NNN157-C-C
Figure A4.8: Scatter plot of NNN157-C-C.
Scatter plot of NNN157-D-C
Figure A4.9: Scatter plot of NNN157-D-C.
Scatter plot of Asp157-D
Figure A4.10: Scatter plot of Asp157-D.
Scatter plot of NNN157-E-A (Fixed)
Figure A4.11: Scatter plot of NNN157-E-A.