In our project, we successfully optimized promoters, signal peptides, strains, and vectors through multiple
rounds of engineering cycles, ultimately constructing a highly efficient system for ICCG expression.The parts we
constructed are shown in the table below.
Basic
| Name/Short Description |
Part Name |
Part Type |
Existing or newly added |
| Mtac274 |
BBa_K5292001 |
Regulator |
New |
| Mtac305 |
BBa_K5292002 |
Regulator |
New |
| Mtac273 |
BBa_K5292003 |
Regulator |
New |
| Mtac290 |
BBa_K5292004 |
Regulator |
New |
| Mtac132 |
BBa_K5292005 |
Regulator |
New |
| Mtac138 |
BBa_K5292006 |
Regulator |
New |
| Mtac137 |
BBa_K5292007 |
Regulator |
New |
| Mtac90 |
BBa_K5292008 |
Regulator |
New |
| Mtac121 |
BBa_K5292009 |
Regulator |
New |
| Mtac141 |
BBa_K52920010 |
Regulator |
New |
| Mtac129 |
BBa_K5292011 |
Regulator |
New |
| Mtac56 |
BBa_K5292012 |
Regulator |
New |
| Mtac66 |
BBa_K5292013 |
Regulator |
New |
| Mtac282 |
BBa_K52920014 |
Regulator |
New |
| Mtac81 |
BBa_K5292015 |
Regulator |
New |
| Mtac95 |
BBa_K5292016 |
Regulator |
New |
| Mtac268 |
BBa_K5292017 |
Regulator |
New |
| Mtac1 |
BBa_K52920018 |
Regulator |
New |
| Mtac27 |
BBa_K5292019 |
Regulator |
New |
| Mtac89 |
BBa_K5292020 |
Regulator |
New |
| Mtac32 |
BBa_K5292021 |
Regulator |
New |
| Mtac30 |
BBa_K52920022 |
Regulator |
New |
| Mtac117 |
BBa_K5292023 |
Regulator |
New |
| Mtac29 |
BBa_K5292024 |
Regulator |
New |
| Mtac23 |
BBa_K5292025 |
Regulator |
New |
| Mtac15 |
BBa_K5292026 |
Regulator |
New |
| Mtac513 |
BBa_K5292027 |
Regulator |
New |
| Mtac433 |
BBa_K5292028 |
Regulator |
New |
| Mtac418 |
BBa_K5292029 |
Regulator |
New |
| Mtac566 |
BBa_K5292030 |
Regulator |
New |
| Mtac417 |
BBa_K5292031 |
Regulator |
New |
| Mtac574 |
BBa_K5292032 |
Regulator |
New |
| Mtac559 |
BBa_K5292033 |
Regulator |
New |
| Mtac386 |
BBa_K5292034 |
Regulator |
New |
| Mtac419 |
BBa_K5292035 |
Regulator |
New |
| Mtac446 |
BBa_K5292036 |
Regulator |
New |
| Mtac464 |
BBa_K5292037 |
Regulator |
New |
| Mtac550 |
BBa_K5292038 |
Regulator |
New |
| Mtac321 |
BBa_K5292039 |
Regulator |
New |
| Mtac387 |
BBa_K5292040 |
Regulator |
New |
| Mtac445 |
BBa_K5292041 |
Regulator |
New |
| Mtac462 |
BBa_K5292042 |
Regulator |
New |
| Mtac567 |
BBa_K5292043 |
Regulator |
New |
| Mtac353 |
BBa_K5292044 |
Regulator |
New |
| Mtac390 |
BBa_K5292045 |
Regulator |
New |
| Mtac575 |
BBa_K5292046 |
Regulator |
New |
| Mtac540 |
BBa_K5292047 |
Regulator |
New |
| Mtac519 |
BBa_K5292048 |
Regulator |
New |
| Mtac447 |
BBa_K5292049 |
Regulator |
New |
| Mtac429 |
BBa_K5292050 |
Regulator |
New |
| Mtac542 |
BBa_K5292051 |
Regulator |
New |
| Mtac451 |
BBa_K5292052 |
Regulator |
New |
| Mtac415 |
BBa_K5292053 |
Regulator |
New |
| Mtac378 |
BBa_K5292054 |
Regulator |
New |
| Mtac361 |
BBa_K5292055 |
Regulator |
New |
| Mtac461 |
BBa_K5292056 |
Regulator |
New |
| Mtac511 |
BBa_K5292057 |
Regulator |
New |
| Mtac374 |
BBa_K5292058 |
Regulator |
New |
| Mtac327 |
BBa_K5292059 |
Regulator |
New |
| Mtac497 |
BBa_K5292060 |
Regulator |
New |
| Mtac662 |
BBa_K5292061 |
Regulator |
New |
| Mtac638 |
BBa_K5292062 |
Regulator |
New |
| Mtac630 |
BBa_K5292063 |
Regulator |
New |
| Mtac669 |
BBa_K5292064 |
Regulator |
New |
| Mtac648 |
BBa_K5292065 |
Regulator |
New |
| Mtac678 |
BBa_K5292066 |
Regulator |
New |
| Mtac728 |
BBa_K5292067 |
Regulator |
New |
| Mtac640 |
BBa_K5292068 |
Regulator |
New |
| Mtac726 |
BBa_K5292069 |
Regulator |
New |
| Mtac623 |
BBa_K5292070 |
Regulator |
New |
| Mtac717 |
BBa_K5292071 |
Regulator |
New |
| Mtac641 |
BBa_K5292072 |
Regulator |
New |
| Mtac718 |
BBa_K5292069 |
Regulator |
New |
| Mtac673 |
BBa_K5292074 |
Regulator |
New |
| Mtac719 |
BBa_K5292075 |
Regulator |
New |
| Mtac660 |
BBa_K5292076 |
Regulator |
New |
| Mtac718 |
BBa_K5292069 |
Regulator |
New |
| Mtac673 |
BBa_K5292074 |
Regulator |
New |
| Mtac719 |
BBa_K5292075 |
Regulator |
New |
| Mtac660 |
BBa_K5292076 |
Regulator |
New |
| Mtac670 |
BBa_K5292077 |
Regulator |
New |
| Mtac622 |
BBa_K5292078 |
Regulator |
New |
| Mtac649 |
BBa_K5292079 |
Regulator |
New |
| Mtac839 |
BBa_K5292080 |
Regulator |
New |
| Mtac635 |
BBa_K5292081 |
Regulator |
New |
| Mtac666 |
BBa_K5292082 |
Regulator |
New |
| Mtac706 |
BBa_K5292083 |
Regulator |
New |
| Mtac642 |
BBa_K5292084 |
Regulator |
New |
| Mtac668 |
BBa_K5292085 |
Regulator |
New |
| Mtac822 |
BBa_K5292086 |
Regulator |
New |
| Mtac723 |
BBa_K5292087 |
Regulator |
New |
| Mtac653 |
BBa_K5292088 |
Regulator |
New |
| DMtac1 |
BBa_K5292101 |
Regulator |
New |
| DMtac3/td>
| BBa_K5292102 |
Regulator |
New |
| DMtac5 |
BBa_K5292103 |
Regulator |
New |
| DMtac7 |
BBa_K5292104 |
Regulator |
New |
| DMtac9 |
BBa_K5292105 |
Regulator |
New |
| DMtac12 |
BBa_K5292106 |
Regulator |
New |
| DMtac13 |
BBa_K5292107 |
Regulator |
New |
| DMtac14 |
BBa_K5292108 |
Regulator |
New |
| DMtac16 |
BBa_K5292109 |
Regulator |
New |
| DMtac19 |
BBa_K5292110 |
Regulator |
New |
| DMtac23 |
BBa_K5292111 |
Regulator |
New |
| DMtac24 |
BBa_K5292112 |
Regulator |
New |
| DMtac29 |
BBa_K5292113 |
Regulator |
New |
| DMtac30 |
BBa_K5292114 |
Regulator |
New |
| DMtac33 |
BBa_K5292115 |
Regulator |
New |
| DMtac34 |
BBa_K5292116 |
Regulator |
New |
| DMtac35 |
BBa_K5292117 |
Regulator |
New |
| DMtac36 |
BBa_K5292118 |
Regulator |
New |
| DMtac42 |
BBa_K5292119 |
Regulator |
New |
| DMtac43 |
BBa_K5292120 |
Regulator |
New |
| DMtac47 |
BBa_K5292121 |
Regulator |
New |
| DMtac51 |
BBa_K5292122 |
Regulator |
New |
| DMtac53 |
BBa_K5292123 |
Regulator |
New |
| DMtac54 |
BBa_K5292124 |
Regulator |
New |
| DMtac55 |
BBa_K5292125 |
Regulator |
New |
| DMtac56 |
BBa_K5292126 |
Regulator |
New |
| DMtac57 |
BBa_K5292127 |
Regulator |
New |
| DMtac59 |
BBa_K5292128 |
Regulator |
New |
| DMtac60 |
BBa_K5292129 |
Regulator |
New |
| DMtac64 |
BBa_K5292130 |
Regulator |
New |
| DMtac65 |
BBa_K5292131 |
Regulator |
New |
| DMtac66 |
BBa_K5292132 |
Regulator |
New |
| DMtac67 |
BBa_K5292133 |
Regulator |
New |
| DMtac68 |
BBa_K5292134 |
Regulator |
New |
| DMtac69 |
BBa_K5292135 |
Regulator |
New |
| DMtac70 |
BBa_K5292136 |
Regulator |
New |
| DMtac72 |
BBa_K5292137 |
Regulator |
New |
| DMtac73 |
BBa_K5292138 |
Regulator |
New |
| AB_pTac |
BBa_J435360 |
Regulator |
Existing |
| SUMO |
BBa_K5292401 |
Tag |
Existing |
| ICCG |
BBa_K5292402 |
Coding |
Existing |
| GFP |
BBa_K5292403 |
Coding |
Existing |
| GSlinker |
BBa_K5292404 |
Protein Domain |
Existing |
| LamB |
K5292300 |
Regulator |
Existing |
| SurA |
K5292301 |
Regulator |
Existing |
| MalE |
K5292302 |
Regulator |
Existing |
| OmpA |
K5292303 |
Regulator |
Existing |
| DsbA |
K5292304 |
Regulator |
Existing |
| TolB |
K5292305 |
Regulator |
Existing |
| PelB |
K5292306 |
Regulator |
Existing |
| MPelB |
K5292307 |
Regulator |
Existing |
| PhoA |
K5292308 |
Regulator |
Existing |
| OmpC |
K5292309 |
Regulator |
Existing |
| OmpF |
K5292310 |
Regulator |
Existing |
| OmpT |
K5292311 |
Regulator |
Existing |
| PhoE |
K5292312 |
Regulator |
Existing |
| nfaA |
K5292313 |
Regulator |
Existing |
| fkpA |
K5292314 |
Regulator |
Existing |
| fedA |
K5292315 |
Regulator |
Existing |
| slt |
K5292316 |
Regulator |
Existing |
| pstS |
K5292317 |
Regulator |
Existing |
| mppA |
K5292318 |
Regulator |
Existing |
| fanG |
K5292319 |
Regulator |
Existing |
| OmpL |
K5292320 |
Regulator |
Existing |
| proX |
K5292321 |
Regulator |
Existing |
| CS3 |
K5292322 |
Regulator |
Existing |
| dppA |
K5292323 |
Regulator |
Existing |
| stal |
K5292324 |
Regulator |
Existing |
| potD |
K5292325 |
Regulator |
Existing |
| pac |
K5292326 |
Regulator |
Existing |
| papA |
K5292327 |
Regulator |
Existing |
| asr |
K5292328 |
Regulator |
Existing |
| iutA |
K5292329 |
Regulator |
Existing |
| hst1 |
K5292330 |
Regulator |
Existing |
| blc1 |
K5292331 |
Regulator |
Existing |
| Fmf7 |
K5292332 |
Regulator |
Existing |
| YtfJ |
K5292333 |
Regulator |
Existing |
| UidC |
K5292334 |
Regulator |
Existing |
| YebF |
K5292335 |
Regulator |
Existing |
| OstA |
K5292336 |
Regulator |
Existing |
| DsbC |
K5292337 |
Regulator |
Existing |
| MnfaA-3 |
K5292339 |
Regulator |
New |
| MnfaA-5 |
K5292341 |
Regulator |
New |
| MnfaA-6 |
K5292342 |
Regulator |
New |
| MnfaA-7 |
K5292343 |
Regulator |
New |
| MnfaA-8 |
K5292344 |
Regulator |
New |
| MnfaA-9 |
K5292345 |
Regulator |
New |
| MnfaA-10 |
K5292346 |
Regulator |
New |
| MnfaA-11 |
K5292347 |
Regulator |
New |
| MnfaA-12 |
K5292348 |
Regulator |
New |
| MnfaA-13 |
K5292349 |
Regulator |
New |
| MnfaA-14 |
K5292350 |
Regulator |
New |
| MnfaA-15 |
K5292351 |
Regulator |
New |
| MnfaA-16 |
K5292352 |
Regulator |
New |
| MnfaA-17 |
K5292353 |
Regulator |
New |
| MnfaA-18 |
K5292354 |
Regulator |
New |
| MnfaA-19 |
K5292355 |
Regulator |
New |
| MnfaA-20 |
K5292356 |
Regulator |
New |
| MnfaA-21 |
K5292357 |
Regulator |
New |
| MnfaA-23 |
K5292359 |
Regulator |
New |
| MnfaA-24 |
K5292360 |
Regulator |
New |
| MnfaA-25 |
K5292361 |
Regulator |
New |
Composite
| Name/Short Description |
Part Name |
Part Type |
Existing or newly added |
| ICCG-GFP |
BBa_K5292405 |
Coding |
Existing |
