In our project, we successfully optimized promoters, signal peptides, strains, and vectors through multiple rounds of engineering cycles, ultimately constructing a highly efficient system for ICCG expression.The parts we constructed are shown in the table below.
| Name/Short Description | Part Name | Part Type | Existing or newly added |
|---|---|---|---|
| Mtac274 | BBa_K5292001 | Regulator | New |
| Mtac305 | BBa_K5292002 | Regulator | New |
| Mtac273 | BBa_K5292003 | Regulator | New |
| Mtac290 | BBa_K5292004 | Regulator | New |
| Mtac132 | BBa_K5292005 | Regulator | New |
| Mtac138 | BBa_K5292006 | Regulator | New |
| Mtac137 | BBa_K5292007 | Regulator | New |
| Mtac90 | BBa_K5292008 | Regulator | New |
| Mtac121 | BBa_K5292009 | Regulator | New |
| Mtac141 | BBa_K52920010 | Regulator | New |
| Mtac129 | BBa_K5292011 | Regulator | New |
| Mtac56 | BBa_K5292012 | Regulator | New |
| Mtac66 | BBa_K5292013 | Regulator | New |
| Mtac282 | BBa_K52920014 | Regulator | New |
| Mtac81 | BBa_K5292015 | Regulator | New |
| Mtac95 | BBa_K5292016 | Regulator | New |
| Mtac268 | BBa_K5292017 | Regulator | New |
| Mtac1 | BBa_K52920018 | Regulator | New |
| Mtac27 | BBa_K5292019 | Regulator | New |
| Mtac89 | BBa_K5292020 | Regulator | New |
| Mtac32 | BBa_K5292021 | Regulator | New |
| Mtac30 | BBa_K52920022 | Regulator | New |
| Mtac117 | BBa_K5292023 | Regulator | New |
| Mtac29 | BBa_K5292024 | Regulator | New |
| Mtac23 | BBa_K5292025 | Regulator | New |
| Mtac15 | BBa_K5292026 | Regulator | New |
| Mtac513 | BBa_K5292027 | Regulator | New |
| Mtac433 | BBa_K5292028 | Regulator | New |
| Mtac418 | BBa_K5292029 | Regulator | New |
| Mtac566 | BBa_K5292030 | Regulator | New |
| Mtac417 | BBa_K5292031 | Regulator | New |
| Mtac574 | BBa_K5292032 | Regulator | New |
| Mtac559 | BBa_K5292033 | Regulator | New |
| Mtac386 | BBa_K5292034 | Regulator | New |
| Mtac419 | BBa_K5292035 | Regulator | New |
| Mtac446 | BBa_K5292036 | Regulator | New |
| Mtac464 | BBa_K5292037 | Regulator | New |
| Mtac550 | BBa_K5292038 | Regulator | New |
| Mtac321 | BBa_K5292039 | Regulator | New |
| Mtac387 | BBa_K5292040 | Regulator | New |
| Mtac445 | BBa_K5292041 | Regulator | New |
| Mtac462 | BBa_K5292042 | Regulator | New |
| Mtac567 | BBa_K5292043 | Regulator | New |
| Mtac353 | BBa_K5292044 | Regulator | New |
| Mtac390 | BBa_K5292045 | Regulator | New |
| Mtac575 | BBa_K5292046 | Regulator | New |
| Mtac540 | BBa_K5292047 | Regulator | New |
| Mtac519 | BBa_K5292048 | Regulator | New |
| Mtac447 | BBa_K5292049 | Regulator | New |
| Mtac429 | BBa_K5292050 | Regulator | New |
| Mtac542 | BBa_K5292051 | Regulator | New |
| Mtac451 | BBa_K5292052 | Regulator | New |
| Mtac415 | BBa_K5292053 | Regulator | New |
| Mtac378 | BBa_K5292054 | Regulator | New |
| Mtac361 | BBa_K5292055 | Regulator | New |
| Mtac461 | BBa_K5292056 | Regulator | New |
| Mtac511 | BBa_K5292057 | Regulator | New |
| Mtac374 | BBa_K5292058 | Regulator | New |
| Mtac327 | BBa_K5292059 | Regulator | New |
| Mtac497 | BBa_K5292060 | Regulator | New |
| Mtac662 | BBa_K5292061 | Regulator | New |
| Mtac638 | BBa_K5292062 | Regulator | New |
| Mtac630 | BBa_K5292063 | Regulator | New |
| Mtac669 | BBa_K5292064 | Regulator | New |
| Mtac648 | BBa_K5292065 | Regulator | New |
| Mtac678 | BBa_K5292066 | Regulator | New |
| Mtac728 | BBa_K5292067 | Regulator | New |
| Mtac640 | BBa_K5292068 | Regulator | New |
| Mtac726 | BBa_K5292069 | Regulator | New |
| Mtac623 | BBa_K5292070 | Regulator | New |
| Mtac717 | BBa_K5292071 | Regulator | New |
| Mtac641 | BBa_K5292072 | Regulator | New |
| Mtac718 | BBa_K5292069 | Regulator | New |
| Mtac673 | BBa_K5292074 | Regulator | New |
| Mtac719 | BBa_K5292075 | Regulator | New |
| Mtac660 | BBa_K5292076 | Regulator | New |
| Mtac718 | BBa_K5292069 | Regulator | New |
| Mtac673 | BBa_K5292074 | Regulator | New |
| Mtac719 | BBa_K5292075 | Regulator | New |
| Mtac660 | BBa_K5292076 | Regulator | New |
| Mtac670 | BBa_K5292077 | Regulator | New |
| Mtac622 | BBa_K5292078 | Regulator | New |
| Mtac649 | BBa_K5292079 | Regulator | New |
| Mtac839 | BBa_K5292080 | Regulator | New |
| Mtac635 | BBa_K5292081 | Regulator | New |
| Mtac666 | BBa_K5292082 | Regulator | New |
| Mtac706 | BBa_K5292083 | Regulator | New |
| Mtac642 | BBa_K5292084 | Regulator | New |
| Mtac668 | BBa_K5292085 | Regulator | New |
| Mtac822 | BBa_K5292086 | Regulator | New |
| Mtac723 | BBa_K5292087 | Regulator | New |
| Mtac653 | BBa_K5292088 | Regulator | New |