Basic Parts

Part	Tested in Lab	Does it Work	Purpose
BBa_K5120000 - pmCHS	✔️	✔️	This part is a basic part that contains the gene for Chalcone Synthase (CHS) from Pueraria mirifica var. lobata, which initiates flavonoid biosynthesis by transforming p-coumaroyl-CoA and malonyl-CoA into naringenin chalcone.
BBa_K5120001 - pmCHR	✔️	✔️	This part is a basic part that contains the gene for Chalcone Reductase (CHR) from Pueraria mirifica var. lobata, which converts 6’-hydroxychalcone into 6’-deoxychalcone, facilitating flavonoid biosynthesis.
BBa_K5120002 - pmIFS	✔️	✔️	This part is a basic part that contains the gene for Isoflavone Synthase (IFS) from Pueraria mirifica var. lobata, which catalyzes the conversion of flavanones into isoflavones, key compounds in isoflavonoid biosynthesis.
BBa_K5120003 - pmHID	✔️	✔️	This part is a basic part that contains the gene for 2-Hydroxyisoflavanone Dehydratase (HID) from Pueraria mirifica var. lobata, which catalyzes the dehydration of 2-hydroxyisoflavonones, facilitating isoflavonoid formation.
BBa_K5120004 - pmCGT	✔️	✔️	This part is a basic part that contains the gene for C-Glycosyltransferase (CGT) from Pueraria mirifica var. lobata, which attaches sugar molecules to flavonoid backbones, enhancing stability and solubility in plants.
BBa_K5120005 - pmCHI	✔️	✔️	This part is a basic part that contains the gene for Chalcone Isomerase (CHI) from Pueraria mirifica var. lobata, which isomerizes chalcones into flavanones, crucial for flavonoid biosynthesis and plant defense.
BBa_K5120006 - pEAQ DEST1 Backbone	✔️	✔️	This part is a basic part for the pEAQ-HT-DEST1 plasmid backbone, a cloning vector designed for high-level transient expression of proteins in plants like Nicotiana benthamiana. It includes essential features such as the CaMV 35S promoter for robust expression, a P19 silencer to mitigate post-transcriptional gene silencing, and a Neo/KanR resistance gene.
BBa_K5120007 - atPfr Promoter			This part is a basic part that contains the gene for the Pfr promoter from Arabidopsis thaliana, which regulates gene expression in response to light, initiating transcription when red light is absorbed.

Composite Parts

Part	Tested in Lab	Does it Work	Purpose
BBa_K5120012 - CHS + pEAQ	✔️	✔️	This part is a composite plasmid designed for the expression of the Chalcone Synthase (CHS) enzyme in plants like Nicotiana benthamiana. This part can be used in Agrobacterium-mediated vector transformation which will make the plant express the CHS gene which catalyzes the first step in flavonoid biosynthesis, converting p-coumaroyl-CoA and malonyl-CoA into naringenin chalcone, a precursor for various flavonoid compounds.
BBa_K5120011 - CHR + pEAQ	✔️	✔️	This part is a composite plasmid designed for the expression of the Chalcone Reductase (CHR) enzyme in plants like Nicotiana benthamiana. This part can be used in Agrobacterium-mediated vector transformation which will make the plant express the CHR gene which reduces chalcones into flavanones, playing a role in isoflavonoid biosynthesis.
BBa_K5120014 - IFS + pEAQ	✔️	✔️	This part is a composite plasmid designed for the expression of Isoflavone Synthase (IFS) in plants like Nicotiana benthamiana. This part can be used in Agrobacterium-mediated vector transformation which will make the plant express the IFS gene which converts flavanones into isoflavones, important compounds in isoflavonoid biosynthesis.
BBa_K5120015 - HID + pEAQ	✔️	✔️	This part is a composite plasmid designed for the expression of Hydroxyisoflavanone Dehydratase (HID) in plants like Nicotiana benthamiana. This part can be used in Agrobacterium-mediated vector transformation which will make the plant express the HID gene which catalyzes the dehydration of hydroxyisoflavonones to form isoflavones, crucial in isoflavonoid biosynthesis.
BBa_K5120016 - CGT + pEAQ	✔️	✔️	This part is a composite plasmid designed for the expression of C-Glycosyltransferase (CGT) in plants like Nicotiana benthamiana, enhancing flavonoid stability and solubility through glycosylation.
BBa_K5120017 - CHI + pEAQ	✔️	✔️	This part is a composite plasmid designed for the expression of Chalcone Isomerase (CHI) in plants like Nicotiana benthamiana. This part can be used in Agrobacterium-mediated vector transformation which will make the plant express the CHI gene which catalyzes the isomerization of chalcones into flavanones, an essential step in flavonoid production.
BBa_K5120018 - Kill Switch + pEAQ			This part is a plasmid designed to initiate cell death in plants upon absorption of red light. This part can be used in Agrobacterium-mediated vector transformation which will make the plant express the Barnase gene upon absorption of red light which induces cell death by degrading RNA, providing a kill switch to contain the modified plant.