Our biosafety committee consists of three experienced research mentors who play a crucial role in overseeing the safety protocols within our laboratory environment. Their responsibilities include meticulously monitoring the conditions of the work area to ensure that all safety standards are consistently met. This involves supervising the correct disposal of laboratory materials, such as Petri dishes, and managing the handling and disposal of liquid waste to prevent any potential contamination.

Beyond their oversight duties, these teachers are deeply committed to fostering a safe and hygienic workspace. They lead targeted safety training sessions tailored for our team members, providing in-depth guidance on best practices for lab sanitation, safe handling of biological materials, and emergency procedures. Their expertise ensures that our workspace remains clean, organized, and compliant with all safety regulations before, during, and after our experiments. Through their proactive involvement, they help create a safe working environment that prioritizes the well-being of all participants and supports the smooth operation of our research activities.

1. When entering the laboratory, you must wear a lab coat, gloves and goggles, and wear a mask when necessary to prevent contact with chemicals and biological samples.

2. Be familiar with the location and use of all safety equipment in the laboratory, such as emergency eyewash, fire extinguisher, safety shower, first aid kit and emergency exit.

3. When performing operations involving pathogens, strains and infectious materials, they must be performed in a biological safety cabinet to ensure that contamination and aerosol diffusion are prevented.

5. After the experiment, waste should be classified and processed. Biological waste must be autoclaved or processed in accordance with biosafety regulations; liquid waste must be neutralized or properly treated before it can be discharged.

6. Eating, drinking or smoking is strictly prohibited in the laboratory to prevent the ingestion of harmful substances or contamination of food.

7. When performing cell culture, bacterial culture and other operations, aseptic operation techniques must be used to prevent cross-contamination of samples and the environment.

8. After the experiment, the laboratory table must be cleaned and disinfected with appropriate disinfectants to ensure the cleanliness and safety of the experimental environment.

9. Avoid making loud noises, running or doing dangerous actions in the laboratory, and maintain good laboratory order and a safe environment.

10. Ensure that the laboratory is well ventilated. If necessary, use a fume hood or exhaust device to handle toxic or volatile chemicals to prevent the accumulation of harmful gases.

11. Before using chemicals, you must understand the properties, potential hazards and emergency treatment measures of chemicals.

12. Pipettes must be used correctly, avoid using mouth suction to prevent sample inhalation and cross contamination.

13. When using centrifuges, electrophoresis instruments, PCR instruments and other equipment, you must strictly follow the operating manual to avoid risks such as electric shock and mechanical damage.

14. Understand the handling measures for common emergencies in the laboratory, such as chemical leaks, biohazard exposure, fire and electrical accidents, to ensure timely and effective responses.

15. Any laboratory accidents or safety hazards must be reported to the laboratory director in a timely manner, and accident records must be kept for subsequent improvement of safety measures.

16. Laboratory members must undergo safety training and master basic safety operation skills before they can perform experimental operations.

17. Be particularly careful when operating needles, blades and glassware. Dispose of sharp objects properly immediately after use to avoid punctures or cuts.

18. Personal items such as bags and coats must not be brought into the laboratory area to avoid contaminating experimental materials or hindering experimental operations.

19. The transportation and storage of biological samples must strictly control temperature and conditions to avoid sample degradation, contamination or diffusion.

20. After the experiment, whether or not you have direct contact with the experimental materials, you must wash your hands thoroughly with soap and water to prevent contaminants from being taken out of the laboratory.

Escherichia coli BL21

Accident reporting protocols should specify the emergency contact number and identify the instructor responsible for overseeing incident response. Proper personal protective gear, including lab coats, gloves, and safety goggles, must be worn at all times to ensure safety in the laboratory.

1. Wear personal protective equipment. Personal protective equipment, including lab coats, gloves and goggles, must be worn when entering the laboratory. When handling toxic, harmful or infectious substances, add protective masks or face shields as needed.

2. Laboratory members must understand and be proficient in the use of safety equipment in the laboratory, such as emergency eyewash, fire extinguisher, safety shower and first aid kit to ensure timely response in the event of an accident.

3. All chemicals and reagents should be clearly labeled, used strictly according to the label and stored in designated areas. Before use, the safety data sheet (SDS) must be read to understand its physical properties, health hazards and emergency treatment measures.

4. Solid waste, liquid waste and sharp objects generated after the experiment must be handled according to category. Biological waste must be autoclaved or properly disinfected, and chemical waste liquid must be neutralized or collected and treated according to laboratory procedures. Random discharge is prohibited.

5. When conducting cell culture, PCR amplification or strain processing, aseptic operation must be strictly implemented to prevent sample contamination and distortion of results. All instruments should be sterilized before use, and operations should be performed on a sterile bench or in a biosafety cabinet.

6. Eating, drinking, smoking or applying makeup is strictly prohibited in the laboratory. Personal items such as food, beverages and cosmetics must not be brought into the experimental area to avoid cross-contamination of experimental samples and individuals.

7. Common instruments such as pipettes, centrifuges, PCR instruments and electrophoresis equipment must be used in accordance with standard operating procedures to avoid mechanical damage, sample loss or contamination caused by misoperation.

8. After the experiment, the laboratory table should be cleaned immediately and wiped with appropriate disinfectants to ensure the sterility and safety of the next experimental operation.

9. When using a pipette, it is strictly forbidden to pipette by mouth suction. A mechanical pipette should be used to prevent contact or ingestion of harmful reagents.

10. When performing operations that may pose safety hazards, at least two experimenters must be present to ensure that someone can provide assistance in the event of an accident.

We are proud to report that our laboratory procedures were conducted without any incidents or safety concerns. While we encountered various challenges and achievements during our experiments, we consistently prioritized safety by strictly following established protocols. Looking ahead, we remain dedicated to maintaining rigorous biosafety standards in all upcoming experiments and projects.