Safety

Our laboratory safety management is in line with the relevant provisions of the current national standard for biochemistry laboratory safety in China. The following are some of the provisions and regulations that are more relevant to our laboratory in the General Requirements for Laboratory Biosafety (Standard No. GB 19489-2008), and the relevant parts of our laboratory.

National standards related content
Risk assessment and risk control
3.1 The laboratory shall establish and maintain risk assessment and risk control procedures to continuously identify and assess risks and implement necessary control measures.
3.1.1 When laboratory activities involve pathogenic biological agents, the laboratory shall conduct a biological risk assessment.
3.1.2 The risks of all proposed activities shall be assessed in advance, including chemical, physical, radiation, electrical, flood, fire, natural disaster, etc.
3.1.3 Risk assessment should be carried out by experienced professionals.
3.1.5 A risk assessment or review of the risk assessment report shall be conducted on a regular basis.
3.1.6 When new laboratory activities are initiated or changes are intended to be made to assessed laboratory activities, a risk assessment should be conducted in advance or again.
3.1.7 The laboratory shall conduct a risk assessment when handling abnormal amounts or engaging in special activities.
3.1.9 When relevant policies, regulations, standards, etc., are changed, the risk assessment shall be re-conducted.
3.1.10 When taking risk control measures, first consider eliminating the source of danger.
4.1 According to the protective measures taken for the biological factors operated, the laboratory biosafety protection level is divided into one, two, three and four.
6.2.7 The biosafety cabinet shall be installed and used according to the design requirements of the product.
6.3.1.1 The laboratory shall clearly distinguish between the auxiliary work area and the protective area.
6.3.3.1 An independent laboratory air supply and exhaust system should be installed.
6.3.5.1 A biosafe autoclave should be installed in the laboratory protected area.
7.1.3 Laboratory management shall be responsible for the design, implementation, maintenance and improvement of the safety management system.
7.14.11 Personnel training plans shall include safety knowledge and skills training.
7.15.2 It shall be ensured that all safety-related laboratory materials are put into use only after they have been inspected or verified to comply with the requirements of the relevant regulations.
7.16.1 The Laboratory shall have policies and procedures for planning, requesting, approving, implementing, monitoring and evaluating laboratory activities.
7.17.1 The Laboratory shall have policies and procedures for housekeeping.
7.18.2 Special protocols for decontamination, cleaning and disinfection of facilities and equipment should be developed in the event of an accident or spill.
7.19.1 The management of laboratory hazardous waste treatment and disposal shall comply with the requirements of national or local regulations and standards.
7.20.1 Policies and procedures for the transport of hazardous materials shall be established.
7.21.1 Policies and procedures for emergency response shall be established

Laboratory risk assessment

In this project, we did not conduct relevant experiments involving pathogenic biological factors (such as pathogenic bacteria, viruses, etc.) other than E. coli. After testing and evaluation in accordance with the regulations of "operating laboratories generally considered to be non-airborne pathogenic biological factors", the relevant risk level is BSL-2, and the laboratory complies with the provisions of Article 6.2 and is qualified to conduct relevant experiments.

Laboratory safety training

Before conducting the experiment, we were trained in strict biosafety regulations and how to deal with emergency situations. The training mainly includes the following contents
1. In terms of daily protection of laboratory personnel, masks and gloves should be strictly worn during relevant experiments, and alcohol disinfection should be carried out after contact with infectious materials.
2. In terms of relevant waste treatment methods, the laboratory sets up a special biohazard waste bin to hold the garbage, and requires and randomly checks to ensure that all wastes that may contain biohazards are discarded in the yellow trash can, and finally disposed of by the relevant qualified processing institutions.
3. In terms of biosafety and disinfection device configuration, the laboratory is equipped with high-pressure steam and ultraviolet disinfection devices, which can disinfect relevant laboratory supplies and consumables according to demand. In addition, the laboratory is also equipped with independent ventilation, emergency flushing device and other related devices
(4) In the laboratory partition, the laboratory has auxiliary work area, protection area and other related functional zones, to enter the building gate of the laboratory needs to be graded and certified, through all the partitions and laboratory work area gates need identity cards or the use of face for personnel and authority authentication, to ensure safety. Each room in the laboratory is equipped with ultraviolet disinfection device for daily disinfection. To enter the clean room for cell experiments from the protected area, it is necessary to pass two buffer rooms, change protective clothing and other personal protective equipment, and carry out careful disinfection to ensure the safety of personnel and experimental samples.
5. In terms of emergency treatment, when toxic and harmful substances spill, it is necessary to immediately report to the head of the laboratory and the instructor, and conduct treatment and safety education according to the situation. The liquid needs to be covered with absorbent paper immediately to prevent further spread. When the waste with infectious hazards spills, in addition to the above steps, it is also necessary to determine the degree of harm at the first time, and spray 75% ethanol or other disinfectant for disinfection.