Attributions

Project Activity	Start Date	End Date	Total Time
Recruit Team Members	17^th November 2023	5^th April 2024	20 weeks
Development of Project Plan	6^th March 2024	5^th April 2024	4 weeks
Wet Laboratory Activities	17^th June 2024	18^th September 2024	13 weeks
Dry Laboratory Activities	17^th June 2024	22^nd July 2024	5 weeks
Building the Team Wiki	17^th June 2024	2^nd October 2024	15 weeks

Team Member Contributions

Name	Role	General Tasks	Specific Tasks
Dr. Andrew Hitchcock	Primary PI	Other	Giving advice on any problems faced with experimental methods and explaining difficult scientific concepts.
Dr. Ian Lidbury	Secondary PI	Other	Giving advice on any problems faced with experimental methods and explaining difficult scientific concepts.
Mario Javier Garcia Duarte	Advisor	Project Administration, Writing, Safety	(1) Training new team members and keeping track of deadlines. (2) Writing - Reviewing/editing content for the wiki. (3) Safety - Filled in iGEM preliminary safety forms.
Isaac White	Advisor	Conceptualisation, Project Administration, Safety	(1) Conceptualisation - helped decide on initial project idea; (2) Project Administration - co-ordinating weekly plans and setting long-term goals; (3) Safety - Giving safety talks and ensuring all team members have read and signed safety forms. Helping manage the team and provide technical support daily.
Brooks J Rady	Advisor	Conceptualisation, Project Administration	(1) Conceptualisation - helped decide on initial project idea; (2) Project Administration - co-ordinating weekly plans and setting long-term goals. (3)
Anirudh Jakhmola	Advisor	Fundraising, Other	(1) Fundraising - Ordering materials for the team; (2) Other - Giving advice on experiments, providing use of sophisticated equipment.
Katie Oxley	Student Leader	Conceptualisation, Investigation, Project Administration, Notebook/Record Keeping, Fundraising	(1) Conceptualisation - helped decide on initial project idea; (2) Investigation - carrying out experiments; (3) Project Administration - deciding on goals for each day; (4) Notebook/Record Keeping - writing up lab activities; (5) Fundraising - contacting university departments about funds.
Grace Marsden	Student Team Member	Notebook/Record Keeping, Investigation, Fundraising, Project Administration	(1) Notebook/Record Keeping - writing up lab activities; (2) Investigation - carrying out experiments; (3) Fundraising - contacting SU to request funds; (4) Project Administration - organising plans for each day; (5) Writing - writing up protocols for Wiki.
Thomas Forster	Student Team Member	Wiki Coding, Investigation, Notebook/Record Keeping, Software, Fundraising	(1) Wiki Coding - designing and putting information up on the Wiki, (2) Investigation - carrying out experiments; (3) Notebook/Record Keeping - writing up lab activities; (4) Software - using AI tools to visualise proteins, (5) Fundraising - contacting companies about sponsorships.
Jacob Lenton	Student Team Member	Public Engagement, Investigation, Notebook/Record Keeping	(1) Public Engagement - contacting farms to request soil samples, organising an educational school visit (2) Investigation - carrying out experiments; (3) Notebook/Record Keeping - writing up lab activities.
Coleen Parakattil	Student Team Member	Public Engagement, Investigation, Notebook/Record Keeping, Visualisation	(1) Public Engagement - designing posts for social media, (2) Investigation - carrying out experiments; (3) Notebook/Record Keeping - writing up lab activities; (4) Visualisation - designing the comic for the wiki.
Saoirse Newhouse	Student Team Member	Writing, Investigation, Notebook/Record Keeping	(1) Writing - writing up protocols for Wiki; (2) Investigation - carrying out experiments; (3) Notebook/Record Keeping - writing up lab activities.
Julia Brzostek	Student Team Member	Public Engagement, Investigation, Hardware, Notebook/Record Keeping, Fundraising, Wiki Coding	(1) Public Engagement - contacting within university about water treatment methods; (2) Investigation - carrying out experiments; (3) Notebook/Record Keeping - writing up lab activities; (4) Fundraising - contacting companies about sponsorships; (5) Wiki Coding - designing and putting information up on the Wiki.
Mary Okunwague Blessing	Student Team Member	Public Engagement, Investigation, Notebook/Record Keeping	(1) Public Engagement - organising an educational school visit & creating a children’s activity book, (2) Investigation - carrying out experiments; (3) Notebook/Record Keeping - writing up lab activities.
Alwin Thomas	Student Team Member	Visualisation, Investigation, Notebook/Record Keeping	(1) Visualisation - designing event posters; (2) Investigation - carrying out experiments; (3) Notebook/Record Keeping - writing up lab activities.

External Contributions

Name	Institution Type	Relationship to team	General Tasks	Specific Tasks
SynBioUK	Industry	Sponsor	Fundraising	Facilitated discussion for our team receiving funds from Full Circle Labs.
Benchling	Industry	Contractor	Software	Provided the amino acid sequences for known enzymes.
AlphaFoldServer	Industry	Contractor	Visualisation	Used given sequences of certain proteins to give a predicted 3D structure.
CBDock-2	Industry	Contractor	Visualisation	Visualised how a specific ligand docked with a protein to make sure which residues were conserved.
MIB2 (metal ion-binding site prediction and modelling server)	Industry	Contractor	Visualisation	Showed where specific ions docked on a protein.
ChimeraX, version 1.6	Industry	Contractor	Visualisation	Carried out molecular visualisation of 3D models of given proteins.
LigandMPNN	Industry	Contractor	Software	Changed amino acid sequences of proteins to create sequences of variants with greater stability.
Xander Arscott-Barber	Academic	Collaborator	Investigation	Assisted with circular dichroism experiments and provided use of machinery and resources for these experiments.
Felix S Morey-Burrows	Academic	Collaborator	Public Engagement	Participated in an online talk on using AI to redesign proteins.
Prof. Mike Williamson	Academic	Collaborator	Public Engagement	Participated in an online talk on using AI to redesign proteins.
Dylan Lewis	Academic	Collaborator	Investigation	Provided use of glass beds for our column device and showed us the protocol for how to use them.
Full Circle Labs	Industry	Sponsor	Fundraising	Provided free DNA sequencing and funded Sheffield’s UK iGEM meetup.
Conservation X Labs	Non-profit	Sponsor	Fundraising	Provided funding for the team’s investigation.
Anna Oxley	Other	Sponsor	Fundraising	Provided free food materials for Sheffield’s UK iGEM meetup.
Campaign & Alumni Relations (CAR)	Non-profit	Sponsor	Fundraising	Donated funds for general iGEM activities.
Integrated DNA Technologies (IDT)	Industry	Sponsor	Fundraising	Provided free DNA sequencing to the team.
Dr Eleni Routoula	Academic	External Advisor	Other	Informed the team on how to use enzymes in industrial processes and certain steps that are required.
Nutrien	Industry	External Advisor	Other	Informed the team about the details of its phosphate mining practices.
Lance Kautz, Florida Department of Environmental Protection	Government	External Advisor	Other	Gave information about the negative impacts of phosphate mining and some ways in which these impacts can be reduced.
Dr Leigh Cassidy	Industry	External Advisor	Other	Provided advice on our project based on her own experience with water filtration technologies.
Sarah Deakin	Other	Sponsor	Other	Provided us with soil samples from Heeley and Meersbrook Allotment Site and provided us with the history of each sample’s plot.
Adam Awadalla	Other	Collaborator	Visualisation	Data visualisation of assay results.

Attributions

Project Timeline

Team Member Contributions

External Contributions