Safety project
Without a doubt, the most precise and intricate tissue in the human body is the brain. Therefore, the physiological function is affected by brain tumors, regardless of whether they are benign or malignant. Recent years have seen an increase in the number of people diagnosed with brain tumors. The primary objective of our study is to develop new substrates for bioluminescent imaging in order to monitor the progression of tumor malignancies. We have always made it a point to put the protection of human life at the forefront of our experimentation process throughout its whole. In light of this, we make every effort to value life, work toward making the world a better place, and steadfastly reject actions that violate moral standards and endanger the safety of human life!
Safety Training
1.protocol for experiment
Professor Du carefully taught us what we should pay attention to during the entire experiment procedure. Following that, we examed the protocol for biological experiment, including the tasks in the cell room and animal facility.
2.Safety manual
We read the safety manual at 06/21/2024
Only finished and accessed the examination can we begin our research procedure in the lab.
3.safety videos
The day before the first day of the chemistry experiment, Professor Du and Doctor Wang showed us a safety video which is mainly about the manipulations of the equipment in the lab.
Safety Policies
Check-in Release Beyond Containment. Throughout the experiment, we refrained from releasing our genetically modified organism (GMO) beyond the laboratory. Initially, all experimental procedures were conducted in the laboratory. Secondly, GMOs were not exchanged between various laboratories due to experimental requirements. Third, upon completion of the experiment, the GMOs were subjected to rigorous processing protocols and humane treatment.
Our team recognizes that GMOs significantly affect ecosystems. Consequently, our team adheres to the values of "being a responsible scientist" and "ensuring the legality of our activities."
White List Policy & No Human Experimentation White List Policy & No Human Experimentation
The experimental microorganisms utilized by our team in the study comply with all regulations, including lentivirus, U87 cells, luciferase-expressing U87 cells, nude mice and mice homozygous for the CAG-luc-eGFP L2G85 transgene.
The lentivirus is classified as a group 1 risk pathogen. U87 cells and luciferase-expressing U87 cells are classified as group 2 risk, fulfilling the biological selection criteria of iGEM. The technique involves the use of lentivirus as a vector for the firefly luciferase gene to infect U87 cells, resulting in luciferase-expressing U87 cells. These cells are subsequently utilized to generate tumors in nude mice and evaluate the efficacy of novel substrates in biological systems.
Animal use
We hold the animal approval by the Ethics Committee of School of Pharmaceutical Sciences, Shandong University, and all animal experiments were conducted in compliance with European guidelines for the care and use of laboratory animals.
Environmental Sample
Mice homozygous for the CAG-luc-eGFP L2G85 transgene (FVB-Tg(CAG-luc,-GFP)L2G85Chco/J) were purchased from the Jackson Laboratory. Balb/c-nu female mice, 6 weeks of age, were obtained from the Animal Center of China Academy of Medical Sciences (Beijing, China). The nude mice and FVB-Tg mice were housed in the Shandong University. This section provides the materials, methodology, and experimental procedures, enabling replication by others.
About our Lab
To mitigate laboratory contamination and other safety issues, our team members underwent a one-week laboratory safety training prior to entering the laboratory. We also made sure the safety signs are clear and confirmed that the experimental equipment are complete to guarantee experiments can be carried out smoothly.
